Restriction map for pF1KB7685
 (Linear) (Six-base) MAPSORT of: KIBB7685.seq  Check: 6139  from: 1  to: 1044



KIBB7685.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     885    1044 
   Size:        114     771     159

  Fragments arranged by size: 

                 771     159     114
 
AfeI AGC'GCT

Cuts at:      0     826    1044 
   Size:        826     218
 
AleI CACnn'nnGTG

Cuts at:      0      90     944    1044 
   Size:         90     854     100

  Fragments arranged by size: 

                 854     100      90
 
AlwNI CAG_nnn'CTG

Cuts at:      0     207     267     322    1044 
   Size:        207      60      55     722

  Fragments arranged by size: 

                 722     207      60      55
 
ApaLI G'TGCA_C

Cuts at:      0     305    1044 
   Size:        305     739
 
ApoI r'AATT_y

Cuts at:      0     589    1019    1044 
   Size:        589     430      25

  Fragments arranged by size: 

                 589     430      25
 
BanII G_rGCy'C

Cuts at:      0     194    1044 
   Size:        194     850
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     705    1044 
   Size:        705     339
 
BbvCI CC'TCA_GC

Cuts at:      0     208     632    1044 
   Size:        208     424     412

  Fragments arranged by size: 

                 424     412     208
 
Bme1580I G_kGCm'C

Cuts at:      0     309    1044 
   Size:        309     735
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      75    1044 
   Size:         75     969
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     367     376     514    1044 
   Size:        344      23       9     138     530

  Fragments arranged by size: 

                 530     344     138      23       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     208     268     632    1044 
   Size:        208      60     364     412

  Fragments arranged by size: 

                 412     364     208      60
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     620    1044 
   Size:        343     277     424

  Fragments arranged by size: 

                 424     343     277
 
BseYI C'CCAG_C

Cuts at:      0     179     488     505    1044 
   Size:        179     309      17     539

  Fragments arranged by size: 

                 539     309     179      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114    1044 
   Size:        114     930
 
BsiHKAI G_wGCw'C

Cuts at:      0     194     309    1044 
   Size:        194     115     735

  Fragments arranged by size: 

                 735     194     115
 
BsmI GAATG_Cn'

Cuts at:      0     223     272    1044 
   Size:        223      49     772

  Fragments arranged by size: 

                 772     223      49
 
Bsp1286I G_dGCh'C

Cuts at:      0     194     309    1044 
   Size:        194     115     735

  Fragments arranged by size: 

                 735     194     115
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     254     296     375    1044 
   Size:        254      42      79     669

  Fragments arranged by size: 

                 669     254      79      42
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12    1044 
   Size:         12    1032
 
BstYI r'GATC_y

Cuts at:      0     351    1044 
   Size:        351     693
 
Bsu36I CC'TnA_GG

Cuts at:      0      20    1044 
   Size:         20    1024
 
BtsI GCAGTG_nn'

Cuts at:      0     341    1044 
   Size:        341     703
 
EarI CTCTTCn'nnn_

Cuts at:      0     545    1044 
   Size:        545     499
 
EcoICRI GAG'CTC

Cuts at:      0     192    1044 
   Size:        192     852
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     344     367     376     514     885    1044 
   Size:        114     230      23       9     138     371     159

  Fragments arranged by size: 

                 371     230     159     138     114      23       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     441    1044 
   Size:        441     603
 
EcoO109I rG'GnC_Cy

Cuts at:      0     387    1044 
   Size:        387     657
 
EcoRI G'AATT_C

Cuts at:      0     589    1019    1044 
   Size:        589     430      25

  Fragments arranged by size: 

                 589     430      25
 
HaeII r_GCGC'y

Cuts at:      0     828    1044 
   Size:        828     216
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      67      99     658     690    1044 
   Size:         67      32     559      32     354

  Fragments arranged by size: 

                 559     354      67      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      90     418     944    1044 
   Size:         90     328     526     100

  Fragments arranged by size: 

                 526     328     100      90
 
MspA1I CmG'CkG

Cuts at:      0     183     492    1044 
   Size:        183     309     552

  Fragments arranged by size: 

                 552     309     183
 
NdeI CA'TA_TG

Cuts at:      0     450    1044 
   Size:        450     594
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     322    1044 
   Size:        322     722
 
PpuMI rG'GwC_Cy

Cuts at:      0     387    1044 
   Size:        387     657
 
PvuII CAG'CTG

Cuts at:      0     183     492    1044 
   Size:        183     309     552

  Fragments arranged by size: 

                 552     309     183
 
SacI G_AGCT'C

Cuts at:      0     194    1044 
   Size:        194     850
 
SfcI C'TryA_G

Cuts at:      0     117    1044 
   Size:        117     927
 
StuI AGG'CCT

Cuts at:      0     981    1044 
   Size:        981      63
 
StyI C'CwwG_G

Cuts at:      0     227     437     594    1044 
   Size:        227     210     157     450

  Fragments arranged by size: 

                 450     227     210     157
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     288    1044 
   Size:        288     756
 
TatI w'GTAC_w

Cuts at:      0     852    1044 
   Size:        852     192
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     449     481    1044 
   Size:        449      32     563

  Fragments arranged by size: 

                 563     449      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     319    1044 
   Size:        319     725
 
XmnI GAAnn'nnTTC

Cuts at:      0     730     898    1044 
   Size:        730     168     146

  Fragments arranged by size: 

                 730     168     146
 

 Enzymes that do cut:

     AcuI     AfeI     AleI    AlwNI    ApaLI     ApoI    BanII     BbsI
    BbvCI Bme1580I     BmrI     BpmI   Bpu10I    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BstXI    BstYI   Bsu36I     BtsI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I
     MslI   MspA1I     NdeI    PflMI    PpuMI    PvuII     SacI     SfcI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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