Restriction map for pF1KB6835
##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcsI          428  AAATTC               [GA]^AATT[CT]
AflIII        147  ACATGT               A^C[GA][CT]GT
AlwNI          51  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          428  AAATTC               [GA]^AATT[CT]
Asp700I       370  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          66  GTGCTC               G[AT]GC[AT]^C
BbsI          473  GAAGAC               GAAGAC(2/6)
BbvCI         214  GCTGAGG              CCTCAGC(-5/-2)
BbvI          450  GCAGC                GCAGC(8/12)
BmyI           66  GTGCTC               G[AGT]GC[ACT]^C
BpmI           57  CTGGAG               CTGGAG(16/14)
BpuAI         473  GAAGAC               GAAGAC(2/6)
BseRI         241  GAGGAG               GAGGAG(10/8)
BsiHKAI        66  GTGCTC               G[AT]GC[AT]^C
BsiYI         210  CCCAGCTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          210  CCCAGCTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I       66  GTGCTC               G[AGT]GC[ACT]^C
BspHI         234  TCATGA               T^CATGA
BspLU11I      147  ACATGT               A^CATGT
BsrDI         298  GCAATG               GCAATG(2/0)
Cac8I          32  GCATGC               GC[ACGT]^[ACGT]GC
Fnu4HI        450  GCAGC                GC^[ACGT]GC
HincII        303  GTTGAC               GT[CT]^[GA]AC
HindII        303  GTTGAC               GT[CT]^[GA]AC
ItaI          450  GCAGC                GC^[ACGT]GC
MaeIII        332  GTCAC                ^GT[ACGT]AC
MseI          361  TTAA                 T^TAA
MwoI           94  GCATTAGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RcaI          234  TCATGA               T^CATGA
SphI           32  GCATGC               GCATG^C
Tru9I         361  TTAA                 T^TAA
TseI          450  GCAGC                G^C[AT]GC
Tsp45I        332  GTCAC                ^GT[GC]AC
TspRI         352  CACTG                CA[GC]TG[ACGT][ACGT]^
XmnI          370  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BsrI          353  ACTGG                ACTGG(1/-1)
              385  ACTGG               
DdeI           69  CTCAG                C^T[ACGT]AG
              215  CTGAG               
EarI           79  GAAGAG               CTCTTC(1/4)
              238  GAAGAG              
EcoT22I        30  ATGCAT               ATGCA^T
              121  ATGCAT              
Ksp632I        79  GAAGAG               CTCTTC(1/4)
              238  GAAGAG              
MspA1I        212  CAGCTG               C[AC]G^C[GT]G
              451  CAGCTG              
NsiI           30  ATGCAT               ATGCA^T
              121  ATGCAT              
NspI           32  GCATGC               [GA]CATG^[CT]
              147  ACATGT              
PleI          168  GACTC                GAGTC(4/5)
               60  GAGTC               
PvuII         212  CAGCTG               CAG^CTG
              451  CAGCTG              
TfiI           73  GATTC                G^A[AT]TC
              496  GATTC               
Tsp509I       199  AATT                 ^AATT
              429  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        BssKI       BstNI       EcoRII      HinfI       
MboII       MnlI        MvaI        NlaIII      ScrFI       
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