Restriction map for pF1KB6835
 (Circular) (Six-base) MAPSORT of: pF1KB6835.seq  Check: 9803  from: 1  to: 3623



pF1KB6835  3623 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AccI GT'mk_AC

Cuts at:    661     661 
   Size:       3623
 
Acc65I G'GTAC_C

Cuts at:    639     639 
   Size:       3623
 
AclI AA'CG_TT

Cuts at:   3463    3463 
   Size:       3623
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1448    1880    2627    2895    1448 
   Size:        432     747     268    2176

  Fragments arranged by size: 

                2176     747     432     268
 
AflIII A'CryG_T

Cuts at:    255     975    2079    3056     255 
   Size:        720    1104     977     822

  Fragments arranged by size: 

                1104     977     822     720
 
AgeI A'CCGG_T

Cuts at:   2011    2011 
   Size:       3623
 
AlwNI CAG_nnn'CTG

Cuts at:    164     928    2495     164 
   Size:        764    1567    1292

  Fragments arranged by size: 

                1567    1292     764
 
ApaLI G'TGCA_C

Cuts at:   2393    2393 
   Size:       3623
 
ApoI r'AATT_y

Cuts at:    536     627    2976    3232     536 
   Size:         91    2349     256     927

  Fragments arranged by size: 

                2349     927     256      91
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3623
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3623
 
AvaI C'yCGr_G

Cuts at:    643     643 
   Size:       3623
 
BamHI G'GATC_C

Cuts at:    648     648 
   Size:       3623
 
BanI G'GyrC_C

Cuts at:    639    1053    1301    1336     639 
   Size:        414     248      35    2926

  Fragments arranged by size: 

                2926     414     248      35
 
BanII G_rGCy'C

Cuts at:    637    1667     637 
   Size:       1030    2593
 
BbeI G_GCGC'C

Cuts at:   1057    1305    1057 
   Size:        248    3375
 
BbsI GAAGACnn'nnnn_

Cuts at:    588     588 
   Size:       3623
 
BbvCI CC'TCA_GC

Cuts at:    323     323 
   Size:       3623
 
BciVI GTATCCnnnnn_n'

Cuts at:   1521    2282    3623    1521 
   Size:        761    1341    1521

  Fragments arranged by size: 

                1521    1341     761
 
BclI T'GATC_A

Cuts at:   1143    1143 
   Size:       3623
 
BfrBI ATG'CAT

Cuts at:    140     231     140 
   Size:         91    3532
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       3623
 
BglII A'GATC_T

Cuts at:   1138    3175    1138 
   Size:       2037    1586
 
BlpI GC'TnA_GC

Cuts at:    735     735 
   Size:       3623
 
Bme1580I G_kGCm'C

Cuts at:   1248    1341    2397    1248 
   Size:         93    1056    2474

  Fragments arranged by size: 

                2474    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    471     835     916    1251    3395     471 
   Size:        364      81     335    2144     699

  Fragments arranged by size: 

                2144     699     364     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1990    1990 
   Size:       3623
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    186     186 
   Size:       3623
 
Bpu10I CC'TnA_GC

Cuts at:    323     323 
   Size:       3623
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    799    1678    2170    2468     799 
   Size:        879     492     298    1954

  Fragments arranged by size: 

                1954     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3327    3327 
   Size:       3623
 
BsaAI yAC'GTr

Cuts at:    976     976 
   Size:       3623
 
BsaBI GATnn'nnATC

Cuts at:      6    1137    3174       6 
   Size:       1131    2037     455

  Fragments arranged by size: 

                2037    1131     455
 
BsaHI Gr'CG_yC

Cuts at:   1054    1302    2004    3500    1054 
   Size:        248     702    1496    1177

  Fragments arranged by size: 

                1496    1177     702     248
 
BsaWI w'CCGG_w

Cuts at:    812    1333    2011    2285    2432    2843     812 
   Size:        521     678     274     147     411    1592

  Fragments arranged by size: 

                1592     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    364     364 
   Size:       3623
 
BseYI C'CCAG_C

Cuts at:    318    1048    1806    2383    2879     318 
   Size:        730     758     577     496    1062

  Fragments arranged by size: 

                1062     758     730     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1165    1211    2419     104 
   Size:       1061      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1061      46
 
BsiHKAI G_wGCw'C

Cuts at:    178     637    1415    1605    2397     178 
   Size:        459     778     190     792    1404

  Fragments arranged by size: 

                1404     792     778     459     190
 
Bsp1286I G_dGCh'C

Cuts at:    178     637    1248    1341    1415    1605    1667    2397     178 
   Size:        459     611      93      74     190      62     730    1404

  Fragments arranged by size: 

                1404     730     611     459     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2843    2843 
   Size:       3623
 
BspHI T'CATG_A

Cuts at:    342    2799     342 
   Size:       2457    1166
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    673     887    1189    1570     673 
   Size:        214     302     381    2726

  Fragments arranged by size: 

                2726     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1915    1969    3453    3618      50 
   Size:       1865      54    1484     165      55

  Fragments arranged by size: 

                1865    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    413    1535     413 
   Size:       1122    2501
 
BsrFI r'CCGG_y

Cuts at:   1621    1802    2011    1621 
   Size:        181     209    3233

  Fragments arranged by size: 

                3233     209     181
 
BssSI C'ACGA_G

Cuts at:   1894    2252    1894 
   Size:        358    3265
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1000    1000 
   Size:       3623
 
BstBI TT'CG_AA

Cuts at:   1984    1984 
   Size:       3623
 
BstYI r'GATC_y

Cuts at:      1     648    1115    1138    1473    1719    2731    2817    2829 
   Size:        647     467      23     335     246    1012      86      12

Cuts at:   2829    3175       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     647     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1573    1754    1917    1573 
   Size:        181     163    3279

  Fragments arranged by size: 

                3279     181     163
 
BtsI GCAGTG_nn'

Cuts at:    866     866 
   Size:       3623
 
DraI TTT'AAA

Cuts at:    622     622 
   Size:       3623
 
DrdI GACnn_nn'nnGTC

Cuts at:   1329    2187    1329 
   Size:        858    2765
 
EaeI y'GGCC_r

Cuts at:   1208    1382    1773    1800    3145    1208 
   Size:        174     391      27    1345    1686

  Fragments arranged by size: 

                1686    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1208    1208 
   Size:       3623
 
EarI CTCTTCn'nnn_

Cuts at:    182     341    1646    1856     182 
   Size:        159    1305     210    1949

  Fragments arranged by size: 

                1949    1305     210     159
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1831    2141    2287    3433    1831 
   Size:        310     146    1146    2021

  Fragments arranged by size: 

                2021    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    635     635 
   Size:       3623
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    186    1448    1880    2627    2895     186 
   Size:       1262     432     747     268     914

  Fragments arranged by size: 

                1262     914     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    762     762 
   Size:       3623
 
EcoRI G'AATT_C

Cuts at:    627     627 
   Size:       3623
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    370     502     623    1642     370 
   Size:        132     121    1019    2351

  Fragments arranged by size: 

                2351    1019     132     121
 
FspI TGC'GCA

Cuts at:   1404    1404 
   Size:       3623
 
HaeII r_GCGC'y

Cuts at:   1057    1305    2327    3306    1057 
   Size:        248    1022     979    1374

  Fragments arranged by size: 

                1374    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1465    1495    1497    1527    1465 
   Size:         30       2      30    3561

  Fragments arranged by size: 

                3561      30      30       2
 
HincII GTy'rAC

Cuts at:    413     662     413 
   Size:        249    3374
 
KasI G'GCGC_C

Cuts at:   1053    1301    1053 
   Size:        248    3375
 
KpnI G_GTAC'C

Cuts at:    643     643 
   Size:       3623
 
MluI A'CGCG_T

Cuts at:   3056    3056 
   Size:       3623
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2294    2478    2294 
   Size:        184    3439
 
MscI TGG'CCA

Cuts at:   1384    1384 
   Size:       3623
 
MslI CAynn'nnrTG

Cuts at:   1612    1739    1612 
   Size:        127    3496
 
MspA1I CmG'CkG

Cuts at:    322     561     734    1048    1408    2421    2666     322 
   Size:        239     173     314     360    1013     245    1279

  Fragments arranged by size: 

                1279    1013     360     314     245     239     173
 
NaeI GCC'GGC

Cuts at:   1804    1804 
   Size:       3623
 
NarI GG'CG_CC

Cuts at:   1054    1302    1054 
   Size:        248    3375
 
NgoMIV G'CCGG_C

Cuts at:   1802    1802 
   Size:       3623
 
NsiI A_TGCA'T

Cuts at:    142     233     142 
   Size:         91    3532
 
NspI r_CATG'y

Cuts at:    144     259     676    1176    2083     144 
   Size:        115     417     500     907    1684

  Fragments arranged by size: 

                1684     907     500     417     115
 
PciI A'CATG_T

Cuts at:    255    2079     255 
   Size:       1824    1799
 
PfoI T'CCnGG_A

Cuts at:    163     473     163 
   Size:        310    3313
 
PmeI GTTT'AAAC

Cuts at:    622     622 
   Size:       3623
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2762    2794    2762 
   Size:         32    3591
 
PstI C_TGCA'G

Cuts at:    670    1355     670 
   Size:        685    2938
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3623
 
PvuII CAG'CTG

Cuts at:    322     561    1048    1408     322 
   Size:        239     487     360    2537

  Fragments arranged by size: 

                2537     487     360     239
 
RsrII CG'GwC_CG

Cuts at:   1818    1818 
   Size:       3623
 
SacI G_AGCT'C

Cuts at:    637     637 
   Size:       3623
 
SalI G'TCGA_C

Cuts at:    660     660 
   Size:       3623
 
SapI GCTCTTCn'nnn_

Cuts at:   1646    1856    1646 
   Size:        210    3413
 
SbfI CC_TGCA'GG

Cuts at:    670     670 
   Size:       3623
 
ScaI AGT'ACT

Cuts at:   3140    3140 
   Size:       3623
 
SfcI C'TryA_G

Cuts at:     33     666    1351    2344    2535      33 
   Size:        633     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     633     191
 
SfoI GGC'GCC

Cuts at:   1055    1303    1055 
   Size:        248    3375
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3623
 
SmaI CCC'GGG

Cuts at:    645     645 
   Size:       3623
 
SmlI C'TyrA_G

Cuts at:    778    1693    2185    2447     778 
   Size:        915     492     262    1954

  Fragments arranged by size: 

                1954     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3623
 
SphI G_CATG'C

Cuts at:    144     676    1176     144 
   Size:        532     500    2591

  Fragments arranged by size: 

                2591     532     500
 
StyI C'CwwG_G

Cuts at:    757    1111     757 
   Size:        354    3269
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1206    1339    1798    2007    1206 
   Size:        133     459     209    2822

  Fragments arranged by size: 

                2822     459     209     133
 
TatI w'GTAC_w

Cuts at:   3138    3138 
   Size:       3623
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3140    3172    3140 
   Size:         32    3591
 
Tth111I GACn'n_nGTC

Cuts at:   1420    1420 
   Size:       3623
 
XbaI T'CTAG_A

Cuts at:    654     654 
   Size:       3623
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1118    1118 
   Size:       3623
 
XmaI C'CCGG_G

Cuts at:    643     643 
   Size:       3623
 
XmnI GAAnn'nnTTC

Cuts at:    482     482 
   Size:       3623
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI     NspI     PciI
     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BcgI    BmgBI     BmtI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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