Restriction map for pF1KE0378
 (Linear) (Six-base) MAPSORT of: KIEE0378.seq  Check: 3778  from: 1  to: 1743



KIEE0378.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0    1120    1743 
   Size:       1120     623
 
AclI AA'CG_TT

Cuts at:      0    1191    1743 
   Size:       1191     552
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     506    1743 
   Size:        506    1237
 
AflIII A'CryG_T

Cuts at:      0    1135    1604    1743 
   Size:       1135     469     139

  Fragments arranged by size: 

                1135     469     139
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     182     185    1743 
   Size:        182       3    1558

  Fragments arranged by size: 

                1558     182       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1336    1743 
   Size:       1336     407
 
ApoI r'AATT_y

Cuts at:      0      16      97     252     337     347     727    1254    1561 
   Size:         16      81     155      85      10     380     527     307

Cuts at:   1561    1743 
   Size:        182

  Fragments arranged by size: 

                 527     380     307     182     155      85      81      16
                  10
 
BanI G'GyrC_C

Cuts at:      0    1434    1743 
   Size:       1434     309
 
BclI T'GATC_A

Cuts at:      0     186    1593    1743 
   Size:        186    1407     150

  Fragments arranged by size: 

                1407     186     150
 
BglII A'GATC_T

Cuts at:      0     927    1743 
   Size:        927     816
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1385    1511    1743 
   Size:       1385     126     232

  Fragments arranged by size: 

                1385     232     126
 
Bpu10I CC'TnA_GC

Cuts at:      0     323    1743 
   Size:        323    1420
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1696    1743 
   Size:       1696      47
 
BsaWI w'CCGG_w

Cuts at:      0     620    1743 
   Size:        620    1123
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     475    1743 
   Size:        475    1268
 
BseYI C'CCAG_C

Cuts at:      0     857    1743 
   Size:        857     886
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1743 
   Size:        974     769
 
BsmI GAATG_Cn'

Cuts at:      0     913    1743 
   Size:        913     830
 
BspEI T'CCGG_A

Cuts at:      0     620    1743 
   Size:        620    1123
 
BsrDI GCAATG_nn'

Cuts at:      0     665    1507    1743 
   Size:        665     842     236

  Fragments arranged by size: 

                 842     665     236
 
BstYI r'GATC_y

Cuts at:      0     927    1332    1743 
   Size:        927     405     411

  Fragments arranged by size: 

                 927     411     405
 
BtsI GCAGTG_nn'

Cuts at:      0     197    1743 
   Size:        197    1546
 
DraI TTT'AAA

Cuts at:      0     416     630     683    1743 
   Size:        416     214      53    1060

  Fragments arranged by size: 

                1060     416     214      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     461    1743 
   Size:        461    1282
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     506    1743 
   Size:        506    1237
 
EcoRI G'AATT_C

Cuts at:      0     252     337    1743 
   Size:        252      85    1406

  Fragments arranged by size: 

                1406     252      85
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     654     686    1743 
   Size:        654      32    1057

  Fragments arranged by size: 

                1057     654      32
 
HindIII A'AGCT_T

Cuts at:      0    1218    1743 
   Size:       1218     525
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     697    1743 
   Size:        697    1046
 
NdeI CA'TA_TG

Cuts at:      0    1473    1743 
   Size:       1473     270
 
NspI r_CATG'y

Cuts at:      0      46      80     331     658    1139    1743 
   Size:         46      34     251     327     481     604

  Fragments arranged by size: 

                 604     481     327     251      46      34
 
PciI A'CATG_T

Cuts at:      0    1135    1743 
   Size:       1135     608
 
PstI C_TGCA'G

Cuts at:      0     174    1743 
   Size:        174    1569
 
SfcI C'TryA_G

Cuts at:      0     170    1121    1743 
   Size:        170     951     622

  Fragments arranged by size: 

                 951     622     170
 
SmlI C'TyrA_G

Cuts at:      0    1675    1743 
   Size:       1675      68
 
SphI G_CATG'C

Cuts at:      0      46    1743 
   Size:         46    1697
 
SwaI ATTT'AAAT

Cuts at:      0     683    1743 
   Size:        683    1060
 
XmnI GAAnn'nnTTC

Cuts at:      0     929    1007    1743 
   Size:        929      78     736

  Fragments arranged by size: 

                 929     736      78
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AlfI    AlwNI     ApoI     BanI
     BclI    BglII     BmrI   Bpu10I    BpuEI    BsaWI    BseRI    BseYI
     BsgI     BsmI    BspEI    BsrDI    BstYI     BtsI     DraI     EarI
  Eco57MI    EcoRI    Hin4I  HindIII     MmeI     NdeI     NspI     PciI
     PstI     SfcI     SmlI     SphI     SwaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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