Restriction map for pF1KB7833
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvI        BcgI        BciVI       BclI        
BcnI        BglI        BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        HpaII       ItaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StyI        SwaI        
TseI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AflII         316  CTTAAG               C^TTAAG
AluI         1080  AGCT                 AG^CT
AlwNI         561  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII        1511  GGTCC                G^G[AT]CC
BbvCI         196  CCTCAGC              CCTCAGC(-5/-2)
BfrI          316  CTTAAG               C^TTAAG
BglII         846  AGATCT               A^GATCT
BsaI          630  GAGACC               GGTCTC(1/5)
BsaJI          71  CCCAGG               C^C[ACGT][ACGT]GG
BsgI         1295  GTGCAG               GTGCAG(16/14)
BsiYI        1502  CCTTTACTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1502  CCTTTACTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         630  GAGAC                GTCTC(1/5)
BstXI         227  CCAGTGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         846  AGATCT               [GA]^GATC[CT]
Cac8I        1413  GCAAGC               GC[ACGT]^[ACGT]GC
DraI         1186  TTTAAA               TTT^AAA
EcoRV         421  GATATC               GAT^ATC
HindIII      1079  AAGCTT               A^AGCTT
MflI          846  AGATCT               [GA]^GATC[CT]
NdeI         1111  CATATG               CA^TATG
SfaNI         126  GATGC                GCATC(5/9)
XhoII         846  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI         1259  GATCC                GGATC(4/5)
               93  GGATC               
AseI          175  ATTAAT               AT^TAAT
             1171  ATTAAT              
AsnI          175  ATTAAT               AT^TAAT
             1171  ATTAAT              
Bsp1407I      687  TGTACA               T^GTACA
             1107  TGTACA              
BsrGI         687  TGTACA               T^GTACA
             1107  TGTACA              
BssKI          72  CCAGG                ^CC[ACGT]GG
             1210  CCTGG               
BstNI          72  CCAGG                CC^[AT]GG
             1210  CCTGG               
Cfr13I       1472  GGCCC                G^G[ACGT]CC
             1511  GGTCC               
EarI         1092  CTCTTC               CTCTTC(1/4)
             1179  CTCTTC              
EcoRII         72  CCAGG                ^CC[AT]GG
             1210  CCTGG               
Ksp632I      1092  CTCTTC               CTCTTC(1/4)
             1179  CTCTTC              
MboII        1093  TCTTC                GAAGA(8/7)
             1180  TCTTC               
MvaI           72  CCAGG                CC^[AT]GG
             1210  CCTGG               
PshBI         175  ATTAAT               AT^TAAT
             1171  ATTAAT              
Sau96I       1472  GGCCC                G^G[ACGT]CC
             1511  GGTCC               
ScrFI          72  CCAGG                CC^[ACGT]GG
             1210  CCTGG               
SfcI          674  CTACAG               C^T[GA][CT]AG
              803  CTATAG              
SspBI         687  TGTACA               T^GTACA
             1107  TGTACA              
StuI         1303  AGGCCT               AGG^CCT
             1434  AGGCCT              
TfiI          144  GAATC                G^A[AT]TC
             1006  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AcsI        AfaI        ApoI        Asp700I     BfaI        
BpmI        BseRI       BsrI        BstF5I      DdeI        
DpnII       FokI        HaeIII      HincII      HindII      
HinfI       HphI        MaeI        MaeIII      MboI        
MnlI        MseI        MslI        NdeII       NlaIII      
NspI        PleI        RsaI        Sau3AI      SmlI        
TaqI        Tru9I       Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com