Restriction map for pF1KB7805
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglII       BlnI        
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRV       EcoT22I     FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NaeI        NarI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SpeI        SphI        Sse8387I    SspI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AciI           98  CCGC                 CCGC(-3/-1)
AflIII       1321  ACATGT               A^C[GA][CT]GT
AlwI          315  GGATC                GGATC(4/5)
ApaI          107  GGGCCC               GGGCC^C
BbsI          556  GTCTTC               GAAGAC(2/6)
BclI          495  TGATCA               T^GATCA
BcnI          464  CCGGG                CC^[GC]GG
BglI          100  GCCCAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           90  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       90  GCTGAGC              GC^T[ACGT]AGC
BpuAI         556  GTCTTC               GAAGAC(2/6)
BsaAI         777  TACGTA               [CT]AC^GT[GA]
BsaI          150  GGTCTC               GGTCTC(1/5)
BseRI         768  CTCCTC               GAGGAG(10/8)
BsgI          930  GTGCAG               GTGCAG(16/14)
BsmI          817  GAATGC               GAATGC(1/-1)
Bsp1407I      113  TGTACA               T^GTACA
BspLU11I     1321  ACATGT               A^CATGT
BspMI          14  ACCTGC               ACCTGC(4/8)
BsrGI         113  TGTACA               T^GTACA
BstEII         39  GGTGACC              G^GT[ACGT]ACC
BstPI          39  GGTGACC              G^GT[ACGT]ACC
CelII          90  GCTGAGC              GC^T[ACGT]AGC
DraI          898  TTTAAA               TTT^AAA
DsaI           23  CCATGG               C^C[GA][CT]GG
EcoO65I        39  GGTGACC              G^GT[ACGT]ACC
EcoT14I        23  CCATGG               C^C[AT][AT]GG
FauI           97  CCCGC                CCCGC(4/6)
FbaI          495  TGATCA               T^GATCA
HapII         464  CCGG                 C^CGG
HpaII         464  CCGG                 C^CGG
MspA1I         88  CAGCTG               C[AC]G^C[GT]G
MspI          464  CCGG                 C^CGG
NciI          464  CCGGG                CC^[GC]GG
NcoI           23  CCATGG               C^CATGG
NdeI         1329  CATATG               CA^TATG
NheI          367  GCTAGC               G^CTAGC
NspI         1321  ACATGT               [GA]CATG^[CT]
PpuMI         168  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII          88  CAGCTG               CAG^CTG
SexAI         146  ACCTGGT              A^CC[AT]GGT
SfcI          138  CTACAG               C^T[GA][CT]AG
SnaBI         777  TACGTA               TAC^GTA
SspBI         113  TGTACA               T^GTACA
StyI           23  CCATGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

Asp700I      1086  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1170  GAAAGCTTTC          
BbvI           87  GCAGC                GCAGC(8/12)
              411  GCAGC               
BsmAI        1545  GAGAC                GTCTC(1/5)
              151  GTCTC               
BsrDI         384  GCAATG               GCAATG(2/0)
              989  GCAATG              
Fnu4HI         87  GCAGC                GC^[ACGT]GC
              411  GCAGC               
ItaI           87  GCAGC                GC^[ACGT]GC
              411  GCAGC               
NlaIII         24  CATG                 CATG^
             1322  CATG                
PleI          161  GACTC                GAGTC(4/5)
             1449  GACTC               
SacI          477  GAGCTC               GAGCT^C
              765  GAGCTC              
StuI          752  AGGCCT               AGG^CCT
             1088  AGGCCT              
TseI           87  GCAGC                G^C[AT]GC
              411  GCAGC               
XmnI         1086  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1170  GAAAGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AspHI       
AvaII       BanII       BfaI        BmyI        BpmI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRI       EcoRII      FokI        
HaeIII      HindIII     HinfI       HphI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       
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