Restriction map for pF1KB7769
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbvCI       BciVI       BclI        
BfrI        BglII       BlnI        BsaBI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstXI       BstZ17I     
ClaI        CpoI        DraI        DraIII      EarI        
Eco47III    EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NarI        
NdeI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AflIII        995  ACACGT               A^C[GA][CT]GT
AgeI          647  ACCGGT               A^CCGGT
ApaI          852  GGGCCC               GGGCC^C
AspHI         370  GTGCTC               G[AT]GC[AT]^C
BbrPI         996  CACGTG               CAC^GTG
BbsI          387  GAAGAC               GAAGAC(2/6)
BcgI           88  CGAGGGTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1247  GCCACACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          483  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      483  GCTCAGC              GC^T[ACGT]AGC
BpuAI         387  GAAGAC               GAAGAC(2/6)
BsaAI         996  CACGTG               [CT]AC^GT[GA]
BsiEI        1336  CGGCCG               CG[GA][CT]^CG
BsiHKAI       370  GTGCTC               G[AT]GC[AT]^C
BsmBI         955  GAGACG               CGTCTC(1/5)
BssSI        1062  CACGAG               CACGAG(-5/-1)
BstAPI        363  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        713  GGTTACC              G^GT[ACGT]ACC
BstPI         713  GGTTACC              G^GT[ACGT]ACC
BstYI         242  AGATCC               [GA]^GATC[CT]
CelII         483  GCTCAGC              GC^T[ACGT]AGC
DrdI          426  GACAGTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1336  CGGCCG               C^GGCCG
EclXI        1336  CGGCCG               C^GGCCG
Eco52I       1336  CGGCCG               C^GGCCG
EcoO65I       713  GGTTACC              G^GT[ACGT]ACC
KspI         1014  CCGCGG               CCGC^GG
MaeII         997  ACGT                 A^CGT
MboII         387  GAAGA                GAAGA(8/7)
MflI          242  AGATCC               [GA]^GATC[CT]
MslI         1060  CACACGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          576  CCATGG               C^CATGG
NheI         1092  GCTAGC               G^CTAGC
PflMI         102  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         647  ACCGGT               A^CCGGT
PleI          433  GAGTC                GAGTC(4/5)
PmaCI         996  CACGTG               CAC^GTG
PmlI          996  CACGTG               CAC^GTG
PshAI         774  GACAGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII        1014  CCGCGG               CCGC^GG
SmaI         1086  CCCGGG               CCC^GGG
SmlI           55  CTTGAG               C^T[CT][GA]AG
StuI          248  AGGCCT               AGG^CCT
TfiI          621  GATTC                G^A[AT]TC
Van91I        102  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         242  AGATCC               [GA]^GATC[CT]
XmaCI        1086  CCCGGG               C^CCGGG
XmaI         1086  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI          778  GGCGTC               G[GA]^CG[CT]C
             1262  GGCGTC              
AfaI          666  GTAC                 GT^AC
              690  GTAC                
AhdI         1024  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1297  GACTGGAAGTC         
AlwI          243  GATCC                GGATC(4/5)
              341  GGATC               
AspEI        1024  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1297  GACTGGAAGTC         
BanI          531  GGCACC               G^G[CT][GA]CC
              572  GGCACC              
BfaI          298  CTAG                 C^TAG
             1093  CTAG                
BsaHI         778  GGCGTC               G[GA]^CG[CT]C
             1262  GGCGTC              
BsaI           46  GAGACC               GGTCTC(1/5)
              702  GGTCTC              
BsaWI         647  ACCGGT               [AT]^CCGG[AT]
             1052  ACCGGA              
BsgI         1078  GTGCAG               GTGCAG(16/14)
             1203  GTGCAG              
BspMI         948  ACCTGC               ACCTGC(4/8)
              112  GCAGGT              
DpnII         243  GATC                 ^GATC
              342  GATC                
DsaI          576  CCATGG               C^C[GA][CT]GG
             1014  CCGCGG              
Eam1105I     1024  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1297  GACTGGAAGTC         
EcoNI         357  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              439  CCTGGGGAAGG         
EcoT14I       182  CCAAGG               C^C[AT][AT]GG
              576  CCATGG              
FauI           74  CCCGC                CCCGC(4/6)
             1199  GCGGG               
Hin1I         778  GGCGTC               G[GA]^CG[CT]C
             1262  GGCGTC              
HinfI         433  GAGTC                G^A[ACGT]TC
              621  GATTC               
MaeI          298  CTAG                 C^TAG
             1093  CTAG                
MboI          243  GATC                 ^GATC
              342  GATC                
NaeI          861  GCCGGC               GCC^GGC
              969  GCCGGC              
NdeII         243  GATC                 ^GATC
              342  GATC                
NgoMIV        861  GCCGGC               G^CCGGC
              969  GCCGGC              
RsaI          666  GTAC                 GT^AC
              690  GTAC                
Sau3AI        243  GATC                 ^GATC
              342  GATC                
StyI          182  CCAAGG               C^C[AT][AT]GG
              576  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AocI        
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DraII       EaeI        Eco81I      
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
ItaI        MaeIII      MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       PpuMI       PstI        PvuII       Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     
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