Restriction map for pF1KB7769
 (Linear) (Six-base) MAPSORT of: KIBB7769.seq  Check: 2321  from: 1  to: 1350



KIBB7769.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     957    1350 
   Size:        957     393
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379    1194    1350 
   Size:        379     815     156

  Fragments arranged by size: 

                 815     379     156
 
AflIII A'CryG_T

Cuts at:      0     995    1350 
   Size:        995     355
 
AgeI A'CCGG_T

Cuts at:      0     647    1350 
   Size:        647     703
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1029    1302    1350 
   Size:       1029     273      48

  Fragments arranged by size: 

                1029     273      48
 
AleI CACnn'nnGTG

Cuts at:      0    1064    1350 
   Size:       1064     286
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     808    1350 
   Size:        808     542
 
AlwNI CAG_nnn'CTG

Cuts at:      0     162     195     222    1280    1350 
   Size:        162      33      27    1058      70

  Fragments arranged by size: 

                1058     162      70      33      27
 
ApaI G_GGCC'C

Cuts at:      0     856    1350 
   Size:        856     494
 
AvaI C'yCGr_G

Cuts at:      0     119     238     798    1086    1350 
   Size:        119     119     560     288     264

  Fragments arranged by size: 

                 560     288     264     119     119
 
BanI G'GyrC_C

Cuts at:      0     531     572    1350 
   Size:        531      41     778

  Fragments arranged by size: 

                 778     531      41
 
BanII G_rGCy'C

Cuts at:      0     412     509     856    1350 
   Size:        412      97     347     494

  Fragments arranged by size: 

                 494     412     347      97
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     394    1350 
   Size:        394     956
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      77     111    1350 
   Size:         77      34    1239

  Fragments arranged by size: 

                1239      77      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1253    1350 
   Size:       1253      97
 
BlpI GC'TnA_GC

Cuts at:      0     484    1350 
   Size:        484     866
 
Bme1580I G_kGCm'C

Cuts at:      0     229     575     856     935    1350 
   Size:        229     346     281      79     415

  Fragments arranged by size: 

                 415     346     281     229      79
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     678     738    1350 
   Size:        678      60     612

  Fragments arranged by size: 

                 678     612      60
 
BmtI G_CTAG'C

Cuts at:      0    1096    1350 
   Size:       1096     254
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     195    1350 
   Size:        195    1155
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     232     522     703     985    1350 
   Size:        217      15     290     181     282     365

  Fragments arranged by size: 

                 365     290     282     217     181      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1350 
   Size:         76    1274
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      40     708    1350 
   Size:         40     668     642

  Fragments arranged by size: 

                 668     642      40
 
BsaAI yAC'GTr

Cuts at:      0     998    1350 
   Size:        998     352
 
BsaHI Gr'CG_yC

Cuts at:      0     779    1263    1350 
   Size:        779     484      87

  Fragments arranged by size: 

                 779     484      87
 
BsaWI w'CCGG_w

Cuts at:      0     647    1052    1350 
   Size:        647     405     298

  Fragments arranged by size: 

                 647     405     298
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     219     249     865     895    1186    1216    1350 
   Size:        219      30     616      30     291      30     134

  Fragments arranged by size: 

                 616     291     219     134      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     298     517     643    1207    1350 
   Size:         73     225     219     126     564     143

  Fragments arranged by size: 

                 564     225     219     143     126      73
 
BseYI C'CCAG_C

Cuts at:      0     753     839    1350 
   Size:        753      86     511

  Fragments arranged by size: 

                 753     511      86
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1099    1224    1350 
   Size:       1099     125     126

  Fragments arranged by size: 

                1099     126     125
 
BsiEI CG_ry'CG

Cuts at:      0    1339    1350 
   Size:       1339      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     374    1350 
   Size:        374     976
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     949    1350 
   Size:        949     401
 
Bsp1286I G_dGCh'C

Cuts at:      0     229     374     412     509     575     856     935    1350 
   Size:        229     145      38      97      66     281      79     415

  Fragments arranged by size: 

                 415     281     229     145      97      79      66      38
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     103     957    1350 
   Size:        103     854     393

  Fragments arranged by size: 

                 854     393     103
 
BsrFI r'CCGG_y

Cuts at:      0     647     861     969    1334    1350 
   Size:        647     214     108     365      16

  Fragments arranged by size: 

                 647     365     214     108      16
 
BssSI C'ACGA_G

Cuts at:      0    1062    1350 
   Size:       1062     288
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     369    1350 
   Size:        369     981
 
BstEII G'GTnAC_C

Cuts at:      0     713    1350 
   Size:        713     637
 
BstYI r'GATC_y

Cuts at:      0     242    1350 
   Size:        242    1108
 
Bsu36I CC'TnA_GG

Cuts at:      0     170     281     420     566    1350 
   Size:        170     111     139     146     784

  Fragments arranged by size: 

                 784     170     146     139     111
 
BtgI C'CryG_G

Cuts at:      0     576    1014    1350 
   Size:        576     438     336

  Fragments arranged by size: 

                 576     438     336
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     691    1350 
   Size:        691     659
 
BtsI GCAGTG_nn'

Cuts at:      0     229     498    1012    1281    1350 
   Size:        229     269     514     269      69

  Fragments arranged by size: 

                 514     269     269     229      69
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     432    1350 
   Size:        432     918
 
EaeI y'GGCC_r

Cuts at:      0     268    1011    1284    1336    1350 
   Size:        268     743     273      52      14

  Fragments arranged by size: 

                 743     273     268      52      14
 
EagI C'GGCC_G

Cuts at:      0    1336    1350 
   Size:       1336      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     232     379     522     703     985    1194    1350 
   Size:        217      15     147     143     181     282     209     156

  Fragments arranged by size: 

                 282     217     209     181     156     147     143      15
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     361     443    1350 
   Size:        361      82     907

  Fragments arranged by size: 

                 907     361      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16     115     448     852    1171    1350 
   Size:         16      99     333     404     319     179

  Fragments arranged by size: 

                 404     333     319     179      99      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     801     833     865     897    1015    1047    1148    1180 
   Size:        801      32      32      32     118      32     101      32

Cuts at:   1180    1288    1320    1350 
   Size:        108      32      30

  Fragments arranged by size: 

                 801     118     108     101      32      32      32      32
                  32      32      30
 
MslI CAynn'nnrTG

Cuts at:      0    1064    1350 
   Size:       1064     286
 
MspA1I CmG'CkG

Cuts at:      0     267     292     488    1016    1214    1227    1260    1311 
   Size:        267      25     196     528     198      13      33      51

Cuts at:   1311    1350 
   Size:         39

  Fragments arranged by size: 

                 528     267     198     196      51      39      33      25
                  13
 
NaeI GCC'GGC

Cuts at:      0     863     971    1350 
   Size:        863     108     379

  Fragments arranged by size: 

                 863     379     108
 
NcoI C'CATG_G

Cuts at:      0     576    1350 
   Size:        576     774
 
NgoMIV G'CCGG_C

Cuts at:      0     861     969    1350 
   Size:        861     108     381

  Fragments arranged by size: 

                 861     381     108
 
NheI G'CTAG_C

Cuts at:      0    1092    1350 
   Size:       1092     258
 
PasI CC'CwG_GG

Cuts at:      0     322     439     708     849     856     916    1350 
   Size:        322     117     269     141       7      60     434

  Fragments arranged by size: 

                 434     322     269     141     117      60       7
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     108    1350 
   Size:        108    1242
 
PmlI CAC'GTG

Cuts at:      0     998    1350 
   Size:        998     352
 
PpuMI rG'GwC_Cy

Cuts at:      0      16     115     448    1350 
   Size:         16      99     333     902

  Fragments arranged by size: 

                 902     333      99      16
 
PshAI GACnn'nnGTC

Cuts at:      0     778    1350 
   Size:        778     572
 
PspOMI G'GGCC_C

Cuts at:      0     852    1350 
   Size:        852     498
 
PstI C_TGCA'G

Cuts at:      0     167     314     381     493     787     954    1276    1350 
   Size:        167     147      67     112     294     167     322      74

  Fragments arranged by size: 

                 322     294     167     167     147     112      74      67
            
 
PvuII CAG'CTG

Cuts at:      0     488    1214    1227    1311    1350 
   Size:        488     726      13      84      39

  Fragments arranged by size: 

                 726     488      84      39      13
 
SacII CC_GC'GG

Cuts at:      0    1017    1350 
   Size:       1017     333
 
SanDI GG'GwC_CC

Cuts at:      0      16    1350 
   Size:         16    1334
 
SfcI C'TryA_G

Cuts at:      0     163     310     377     489     783     950    1272    1350 
   Size:        163     147      67     112     294     167     322      78

  Fragments arranged by size: 

                 322     294     167     163     147     112      78      67
            
 
SmaI CCC'GGG

Cuts at:      0    1088    1350 
   Size:       1088     262
 
SmlI C'TyrA_G

Cuts at:      0      55    1350 
   Size:         55    1295
 
StuI AGG'CCT

Cuts at:      0     250    1350 
   Size:        250    1100
 
StyI C'CwwG_G

Cuts at:      0     182     576    1350 
   Size:        182     394     774

  Fragments arranged by size: 

                 774     394     182
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     243     626     630    1350 
   Size:        243     383       4     720

  Fragments arranged by size: 

                 720     383     243       4
 
TatI w'GTAC_w

Cuts at:      0     665    1350 
   Size:        665     685
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     219     251     512     544    1186    1218    1350 
   Size:        219      32     261      32     642      32     132

  Fragments arranged by size: 

                 642     261     219     132      32      32      32
 
XmaI C'CCGG_G

Cuts at:      0    1086    1350 
   Size:       1086     264
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI
     ApaI     AvaI     BanI    BanII     BbsI     BcgI     BglI     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrFI    BssSI   BstAPI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI  Eco57MI
    EcoNI EcoO109I    Hin4I     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NheI     PasI    PflMI     PmlI    PpuMI    PshAI   PspOMI     PstI
    PvuII    SacII    SanDI     SfcI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AloI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI    BciVI     BclI    BfrBI    BglII    BmgBI   Bpu10I    BsaBI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BstBI    BstXI  BstZ17I     ClaI    CspCI     DraI   DraIII     EarI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NarI     NdeI     NotI     NruI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PpiI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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