Restriction map for pF1KB6814
##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
ItaI        KasI        KpnI        KspI        MaeII       
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        TseI        Tth111I     
TthHB8I     XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AlwI          126  GGATC                GGATC(4/5)
AspHI         492  GAGCTC               G[AT]GC[AT]^C
BanII         492  GAGCTC               G[GA]GC[CT]^C
BbsI          222  GAAGAC               GAAGAC(2/6)
BglII         278  AGATCT               A^GATCT
BmyI          492  GAGCTC               G[AGT]GC[ACT]^C
BpuAI         222  GAAGAC               GAAGAC(2/6)
BsaBI         139  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       492  GAGCTC               G[AT]GC[AT]^C
BsiYI         112  CCAGGATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          112  CCAGGATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      492  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I      517  TGTACA               T^GTACA
BsrGI         517  TGTACA               T^GTACA
BstF5I        195  CATCC                GGATG(2/0)
BstYI         278  AGATCT               [GA]^GATC[CT]
DdeI          282  CTCAG                C^T[ACGT]AG
FokI          195  CATCC                GGATG(9/13)
HinfI         357  GATTC                G^A[ACGT]TC
HphI          432  TCACC                GGTGA(8/7)
MamI          139  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          278  AGATCT               [GA]^GATC[CT]
MwoI          418  GCTGTTCTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          454  CATATG               CA^TATG
PflMI         112  CCAGGATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          492  GAGCTC               GAGCT^C
SfcI          524  CTATAG               C^T[GA][CT]AG
SmlI          331  CTTGAG               C^T[CT][GA]AG
SspBI         517  TGTACA               T^GTACA
TfiI          357  GATTC                G^A[AT]TC
TspRI         218  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        112  CCAGGATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          147  TCTAGA               T^CTAGA
XhoII         278  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          320  AAATTC               [GA]^AATT[CT]
              505  AAATTT              
ApoI          320  AAATTC               [GA]^AATT[CT]
              505  AAATTT              
BsmAI         446  GAGAC                GTCTC(1/5)
              459  GAGAC               
BspHI         404  TCATGA               T^CATGA
              533  TCATGA              
BsrI           55  ACTGG                ACTGG(1/-1)
              481  CCAGT               
BssKI          25  CCTGG                ^CC[ACGT]GG
              112  CCAGG               
BstNI          25  CCTGG                CC^[AT]GG
              112  CCAGG               
BstXI          38  CCAAGCAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              346  CCAAGTGGCTGG        
DpnII         127  GATC                 ^GATC
              279  GATC                
EarI          443  GAAGAG               CTCTTC(1/4)
              487  GAAGAG              
EcoRII         25  CCTGG                ^CC[AT]GG
              112  CCAGG               
Ksp632I       443  GAAGAG               CTCTTC(1/4)
              487  GAAGAG              
MboI          127  GATC                 ^GATC
              279  GATC                
MnlI           22  CCTC                 CCTC(7/6)
              181  CCTC                
MseI          370  TTAA                 T^TAA
              467  TTAA                
MslI          433  CACCAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              450  CACCCATATG          
MvaI           25  CCTGG                CC^[AT]GG
              112  CCAGG               
NdeII         127  GATC                 ^GATC
              279  GATC                
NlaIV         172  GGTTCC               GG[ACGT]^[ACGT]CC
              342  GGAACC              
RcaI          404  TCATGA               T^CATGA
              533  TCATGA              
Sau3AI        127  GATC                 ^GATC
              279  GATC                
ScrFI          25  CCTGG                CC^[ACGT]GG
              112  CCAGG               
Tru9I         370  TTAA                 T^TAA
              467  TTAA                
Tsp45I         78  GTCAC                ^GT[GC]AC
               93  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

AfaI        AluI        BfaI        MaeI        MaeIII      
MboII       NlaIII      RsaI        Tsp509I     
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