Restriction map for
pF1KB9772
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AfaI AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BciVI BcnI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII
BstPI BstUI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT14I EcoT22I
Fnu4HI FseI FspI HapII HgaI
Hin1I HpaII ItaI KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SbfI ScaI SfaNI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StyI SwaI TseI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 380 AACGTT AA^CGTT
Alw44I 447 GTGCAC G^TGCAC
ApaLI 447 GTGCAC G^TGCAC
AseI 496 ATTAAT AT^TAAT
AsnI 496 ATTAAT AT^TAAT
AspHI 447 GTGCAC G[AT]GC[AT]^C
AvaII 1367 GGACC G^G[AT]CC
BalI 23 TGGCCA TGG^CCA
BclI 1020 TGATCA T^GATCA
BglII 919 AGATCT A^GATCT
BmyI 447 GTGCAC G[AGT]GC[ACT]^C
BsiHKAI 447 GTGCAC G[AT]GC[AT]^C
BsmFI 516 GGGAC GGGAC(10/14)
Bsp1286I 447 GTGCAC G[AGT]GC[ACT]^C
BspHI 1611 TCATGA T^CATGA
BspLU11I 285 ACATGT A^CATGT
BsrDI 243 GCAATG GCAATG(2/0)
Bst1107I 605 GTATAC GTA^TAC
BstZ17I 605 GTATAC GTA^TAC
CfoI 1376 GCGC GCG^C
DraI 942 TTTAAA TTT^AAA
DraII 1132 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 23 TGGCCA [CT]^GGCC[GA]
EcoO109I 1132 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 35 GATATC GAT^ATC
FauI 1574 GCGGG CCCGC(4/6)
FbaI 1020 TGATCA T^GATCA
HaeII 1375 AGCGCC [GA]GCGC^[CT]
HhaI 1376 GCGC GCG^C
HinP1I 1376 GCGC G^CGC
HpaI 1435 GTTAAC GTT^AAC
MluNI 23 TGGCCA TGG^CCA
MscI 23 TGGCCA TGG^CCA
NdeI 856 CATATG CA^TATG
PshBI 496 ATTAAT AT^TAAT
Psp1406I 380 AACGTT AA^CGTT
RcaI 1611 TCATGA T^CATGA
SapI 42 GCTCTTC GCTCTTC(1/4)
SexAI 748 ACCAGGT A^CC[AT]GGT
SspI 682 AATATT AAT^ATT
StuI 1160 AGGCCT AGG^CCT
TfiI 195 GATTC G^A[AT]TC
Tsp45I 476 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 337 GTAGAC GT^[AC][GT]AC
605 GTATAC
AflII 277 CTTAAG C^TTAAG
846 CTTAAG
BamHI 963 GGATCC G^GATCC
1451 GGATCC
BbrPI 1392 CACGTG CAC^GTG
1428 CACGTG
BfaI 86 CTAG C^TAG
917 CTAG
BfrI 277 CTTAAG C^TTAAG
846 CTTAAG
BsaAI 1392 CACGTG [CT]AC^GT[GA]
1428 CACGTG
BsgI 352 CTGCAC GTGCAG(16/14)
94 GTGCAG
BsmI 889 GAATGC GAATGC(1/-1)
1338 GCATTC
BssKI 749 CCAGG ^CC[ACGT]GG
1314 CCTGG
BstBI 1028 TTCGAA TT^CGAA
1604 TTCGAA
BstF5I 165 GGATG GGATG(2/0)
1629 GGATG
BstNI 749 CCAGG CC^[AT]GG
1314 CCTGG
Cac8I 1107 GCATGC GC[ACGT]^[ACGT]GC
1623 GCATGC
Cfr13I 1133 GGCCC G^G[ACGT]CC
1367 GGACC
EarI 43 CTCTTC CTCTTC(1/4)
79 GAAGAG
EcoNI 156 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
743 CCTTCACCAGG
EcoRI 1031 GAATTC G^AATTC
1283 GAATTC
EcoRII 749 CCAGG ^CC[AT]GG
1314 CCTGG
FokI 165 GGATG GGATG(9/13)
1629 GGATG
HindIII 113 AAGCTT A^AGCTT
461 AAGCTT
Ksp632I 43 CTCTTC CTCTTC(1/4)
79 GAAGAG
MaeI 86 CTAG C^TAG
917 CTAG
MvaI 749 CCAGG CC^[AT]GG
1314 CCTGG
NspV 1028 TTCGAA TT^CGAA
1604 TTCGAA
PleI 478 GACTC GAGTC(4/5)
82 GAGTC
PmaCI 1392 CACGTG CAC^GTG
1428 CACGTG
PmlI 1392 CACGTG CAC^GTG
1428 CACGTG
Sau96I 1133 GGCCC G^G[ACGT]CC
1367 GGACC
ScrFI 749 CCAGG CC^[ACGT]GG
1314 CCTGG
SfuI 1028 TTCGAA TT^CGAA
1604 TTCGAA
SmlI 277 CTTAAG C^T[CT][GA]AG
846 CTTAAG
SphI 1107 GCATGC GCATG^C
1623 GCATGC
TaqI 1029 TCGA T^CGA
1605 TCGA
TthHB8I 1029 TCGA T^CGA
1605 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
ApoI Asp700I BpmI BsiYI BslI
BsmAI BsrI BstYI DdeI DpnII
HaeIII HincII HindII HinfI HphI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI NdeII NlaIII
NlaIV NspI Sau3AI Tru9I Tsp509I
TspRI XhoII XmnI