Restriction map for pF1KB7856
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AgeI        AhdI        Alw44I      AlwI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BseAI       BsiWI       BsmBI       
BsmFI       BsoBI       BspDI       BspEI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DrdI        Eam1105I    Eco47III    
EcoNI       EcoO65I     EcoRV       EcoT22I     FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PstI        PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          351  GTATAC               GT^[AC][GT]AC
AclI         1867  AACGTT               AA^CGTT
AlwNI         343  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          238  CCTAAGG              CC^T[ACGT]AGG
BanI          186  GGCACC               G^G[CT][GA]CC
BclI          765  TGATCA               T^GATCA
BcnI           24  CCCGG                CC^[GC]GG
BsaAI         674  TACGTG               [CT]AC^GT[GA]
BsaI          692  GAGACC               GGTCTC(1/5)
BseRI         181  GAGGAG               GAGGAG(10/8)
BsiEI           8  CGGCCG               CG[GA][CT]^CG
BsmI         1726  GAATGC               GAATGC(1/-1)
Bsp1407I     1353  TGTACA               T^GTACA
BsrGI        1353  TGTACA               T^GTACA
Bst1107I      351  GTATAC               GTA^TAC
BstF5I        177  GGATG                GGATG(2/0)
BstZ17I       351  GTATAC               GTA^TAC
Bsu36I        238  CCTAAGG              CC^T[ACGT]AGG
DraIII        118  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          336  CCATGG               C^C[GA][CT]GG
EaeI            8  CGGCCG               [CT]^GGCC[GA]
EagI            8  CGGCCG               C^GGCCG
EarI         1697  GAAGAG               CTCTTC(1/4)
EclXI           8  CGGCCG               C^GGCCG
Eco52I          8  CGGCCG               C^GGCCG
Eco81I        238  CCTAAGG              CC^T[ACGT]AGG
FauI          216  GCGGG                CCCGC(4/6)
FbaI          765  TGATCA               T^GATCA
FokI          177  GGATG                GGATG(9/13)
HindIII       653  AAGCTT               A^AGCTT
HinfI         234  GATTC                G^A[ACGT]TC
Ksp632I      1697  GAAGAG               CTCTTC(1/4)
NciI           24  CCCGG                CC^[GC]GG
NcoI          336  CCATGG               C^CATGG
NdeI         1385  CATATG               CA^TATG
Psp1406I     1867  AACGTT               AA^CGTT
PvuII         471  CAGCTG               CAG^CTG
SfaNI         126  GATGC                GCATC(5/9)
SmlI          294  CTTGAG               C^T[CT][GA]AG
SspBI        1353  TGTACA               T^GTACA
TfiI          234  GATTC                G^A[AT]TC
XcmI          593  CCAAGAGTGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BbsI          261  GAAGAC               GAAGAC(2/6)
               86  GTCTTC              
BpuAI         261  GAAGAC               GAAGAC(2/6)
               86  GTCTTC              
BspHI        1302  TCATGA               T^CATGA
             1311  TCATGA              
BsrDI         443  GCAATG               GCAATG(2/0)
             1562  GCAATG              
DraII         242  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1212  AGGGCCT             
EcoO109I      242  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1212  AGGGCCT             
EcoT14I       324  CCAAGG               C^C[AT][AT]GG
              336  CCATGG              
MspA1I        214  CAGCGG               C[AC]G^C[GT]G
              471  CAGCTG              
MwoI          405  GCACCAGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              443  GCAATGGGGGC         
NlaIV          21  GGCCCC               GG[ACGT]^[ACGT]CC
              186  GGCACC              
RcaI         1302  TCATGA               T^CATGA
             1311  TCATGA              
SfcI          753  CTGTAG               C^T[GA][CT]AG
              825  CTATAG              
StyI          324  CCAAGG               C^C[AT][AT]GG
              336  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
ApoI        Asp700I     AspHI       BanII       BbvI        
BfaI        BglII       BmyI        BpmI        BsaJI       
BsaWI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspLU11I    BsrI        BssKI       
BstNI       BstYI       Cfr13I      DdeI        DpnII       
EcoRI       EcoRII      Fnu4HI      HaeIII      HapII       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        NdeII       
NlaIII      NspI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       StuI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       XmnI        
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