Restriction map for pF1KB7856
 (Linear) (Six-base) MAPSORT of: KIBB7856.seq  Check: 2333  from: 1  to: 1881



KIBB7856.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AccI GT'mk_AC

Cuts at:      0     352    1881 
   Size:        352    1529
 
AclI AA'CG_TT

Cuts at:      0    1868    1881 
   Size:       1868      13
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     535     770    1881 
   Size:        420     115     235    1111

  Fragments arranged by size: 

                1111     420     235     115
 
AflIII A'CryG_T

Cuts at:      0     458     669     675     716     802    1881 
   Size:        458     211       6      41      86    1079

  Fragments arranged by size: 

                1079     458     211      86      41       6
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     578    1881 
   Size:        578    1303
 
AlwNI CAG_nnn'CTG

Cuts at:      0     348    1881 
   Size:        348    1533
 
ApoI r'AATT_y

Cuts at:      0     860    1028    1339    1784    1828    1881 
   Size:        860     168     311     445      44      53

  Fragments arranged by size: 

                 860     445     311     168      53      44
 
BanI G'GyrC_C

Cuts at:      0     186    1881 
   Size:        186    1695
 
BanII G_rGCy'C

Cuts at:      0     838    1594    1762    1881 
   Size:        838     756     168     119

  Fragments arranged by size: 

                 838     756     168     119
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      79     268    1881 
   Size:         79     189    1613

  Fragments arranged by size: 

                1613     189      79
 
BclI T'GATC_A

Cuts at:      0     765    1881 
   Size:        765    1116
 
BglII A'GATC_T

Cuts at:      0     709    1255    1297    1881 
   Size:        709     546      42     584

  Fragments arranged by size: 

                 709     584     546      42
 
BmgBI CAC'GTC

Cuts at:      0      54    1881 
   Size:         54    1827
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     283     645    1881 
   Size:        283     362    1236

  Fragments arranged by size: 

                1236     362     283
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     224    1881 
   Size:        224    1657
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     188     890     974    1310    1478    1562    1578 
   Size:        151      37     702      84     336     168      84      16

Cuts at:   1578    1881 
   Size:        303

  Fragments arranged by size: 

                 702     336     303     168     151      84      84      37
                  16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315    1881 
   Size:        315    1566
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     686    1881 
   Size:        686    1195
 
BsaAI yAC'GTr

Cuts at:      0     676    1881 
   Size:        676    1205
 
BsaWI w'CCGG_w

Cuts at:      0    1190    1624    1708    1881 
   Size:       1190     434      84     173

  Fragments arranged by size: 

                1190     434     173      84
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     632     662    1881 
   Size:        632      30    1219

  Fragments arranged by size: 

                1219     632      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     196    1881 
   Size:        196    1685
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     459     620     741     993    1077    1161    1413    1665 
   Size:        459     161     121     252      84      84     252     252

Cuts at:   1665    1881 
   Size:        216

  Fragments arranged by size: 

                 459     252     252     252     216     161     121      84
                  84
 
BsiEI CG_ry'CG

Cuts at:      0      11    1881 
   Size:         11    1870
 
BsiHKAI G_wGCw'C

Cuts at:      0     205     838    1594    1762    1881 
   Size:        205     633     756     168     119

  Fragments arranged by size: 

                 756     633     205     168     119
 
BsmI GAATG_Cn'

Cuts at:      0    1732    1881 
   Size:       1732     149
 
Bsp1286I G_dGCh'C

Cuts at:      0     205     838    1594    1762    1881 
   Size:        205     633     756     168     119

  Fragments arranged by size: 

                 756     633     205     168     119
 
BspHI T'CATG_A

Cuts at:      0    1302    1311    1881 
   Size:       1302       9     570

  Fragments arranged by size: 

                1302     570       9
 
BsrDI GCAATG_nn'

Cuts at:      0     450    1569    1881 
   Size:        450    1119     312

  Fragments arranged by size: 

                1119     450     312
 
BsrGI T'GTAC_A

Cuts at:      0    1353    1881 
   Size:       1353     528
 
BstYI r'GATC_y

Cuts at:      0     709    1255    1297    1881 
   Size:        709     546      42     584

  Fragments arranged by size: 

                 709     584     546      42
 
BstZ17I GTA'TAC

Cuts at:      0     353    1881 
   Size:        353    1528
 
Bsu36I CC'TnA_GG

Cuts at:      0     239    1881 
   Size:        239    1642
 
BtgI C'CryG_G

Cuts at:      0     336    1881 
   Size:        336    1545
 
BtsI GCAGTG_nn'

Cuts at:      0     608     729     981    1065    1149    1401    1653    1737 
   Size:        608     121     252      84      84     252     252      84

Cuts at:   1737    1881 
   Size:        144

  Fragments arranged by size: 

                 608     252     252     252     144     121      84      84
                  84
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1551    1586    1881 
   Size:       1551      35     295

  Fragments arranged by size: 

                1551     295      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     123    1881 
   Size:        123    1758
 
EaeI y'GGCC_r

Cuts at:      0       8    1881 
   Size:          8    1873
 
EagI C'GGCC_G

Cuts at:      0       8    1881 
   Size:          8    1873
 
EarI CTCTTCn'nnn_

Cuts at:      0    1692    1881 
   Size:       1692     189
 
EcoICRI GAG'CTC

Cuts at:      0     836    1592    1760    1881 
   Size:        836     756     168     121

  Fragments arranged by size: 

                 836     756     168     121
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     188     420     535     770     890     974    1310 
   Size:        151      37     232     115     235     120      84     336

Cuts at:   1310    1478    1562    1578    1881 
   Size:        168      84      16     303

  Fragments arranged by size: 

                 336     303     235     232     168     151     120     115
                  84      84      37      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     243    1213    1881 
   Size:        243     970     668

  Fragments arranged by size: 

                 970     668     243
 
EcoRI G'AATT_C

Cuts at:      0     860    1028    1784    1881 
   Size:        860     168     756      97

  Fragments arranged by size: 

                 860     756     168      97
 
HindIII A'AGCT_T

Cuts at:      0     653    1881 
   Size:        653    1228
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     402    1293    1636    1871    1881 
   Size:        402     891     343     235      10

  Fragments arranged by size: 

                 891     402     343     235      10
 
MslI CAynn'nnrTG

Cuts at:      0     674     887    1316    1389    1881 
   Size:        674     213     429      73     492

  Fragments arranged by size: 

                 674     492     429     213      73
 
MspA1I CmG'CkG

Cuts at:      0     216     473    1881 
   Size:        216     257    1408

  Fragments arranged by size: 

                1408     257     216
 
NcoI C'CATG_G

Cuts at:      0     336    1881 
   Size:        336    1545
 
NdeI CA'TA_TG

Cuts at:      0    1386    1881 
   Size:       1386     495
 
NspI r_CATG'y

Cuts at:      0     368     421     462     673     720     806    1881 
   Size:        368      53      41     211      47      86    1075

  Fragments arranged by size: 

                1075     368     211      86      53      47      41
 
PasI CC'CwG_GG

Cuts at:      0      30     156    1881 
   Size:         30     126    1725

  Fragments arranged by size: 

                1725     126      30
 
PciI A'CATG_T

Cuts at:      0     458     669     716     802    1881 
   Size:        458     211      47      86    1079

  Fragments arranged by size: 

                1079     458     211      86      47
 
PfoI T'CCnGG_A

Cuts at:      0      70    1881 
   Size:         70    1811
 
PvuII CAG'CTG

Cuts at:      0     473    1881 
   Size:        473    1408
 
SacI G_AGCT'C

Cuts at:      0     838    1594    1762    1881 
   Size:        838     756     168     119

  Fragments arranged by size: 

                 838     756     168     119
 
SfcI C'TryA_G

Cuts at:      0     753     825    1881 
   Size:        753      72    1056

  Fragments arranged by size: 

                1056     753      72
 
SmlI C'TyrA_G

Cuts at:      0     294    1881 
   Size:        294    1587
 
StuI AGG'CCT

Cuts at:      0     963    1047    1131    1467    1551    1635    1719    1803 
   Size:        963      84      84     336      84      84      84      84

Cuts at:   1803    1881 
   Size:         78

  Fragments arranged by size: 

                 963     336      84      84      84      84      84      84
                  78
 
StyI C'CwwG_G

Cuts at:      0     324     336    1881 
   Size:        324      12    1545

  Fragments arranged by size: 

                1545     324      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     148    1881 
   Size:        148    1733
 
TatI w'GTAC_w

Cuts at:      0    1353    1881 
   Size:       1353     528
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     632     664    1881 
   Size:        632      32    1217

  Fragments arranged by size: 

                1217     632      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     600    1881 
   Size:        600    1281
 
XmnI GAAnn'nnTTC

Cuts at:      0     433     499     526     655    1881 
   Size:        433      66      27     129    1226

  Fragments arranged by size: 

                1226     433     129      66      27
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AlfI    AlwNI     ApoI     BanI
    BanII     BbsI     BclI    BglII    BmgBI     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrGI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI    CspCI   DraIII     EaeI     EagI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI  HindIII     MmeI     MslI   MspA1I
     NcoI     NdeI     NspI     PasI     PciI     PfoI    PvuII     SacI
     SfcI     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BlpI Bme1580I     BmtI   Bpu10I    BsaBI    BsaHI    BseYI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BtgZI     ClaI     DraI     DrdI     EciI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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