Restriction map for pF1KB7717
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        MseI        NarI        NcoI        
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     Van91I      
XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AhdI          718  GACCGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         525  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         718  GACCGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         699  GTGCTC               G[AT]GC[AT]^C
AvaI          452  CCCGGG               C^[CT]CG[GA]G
BalI          253  TGGCCA               TGG^CCA
BanI         1142  GGTGCC               G^G[CT][GA]CC
BanII         678  GAGCCC               G[GA]GC[CT]^C
BbvCI         645  CCTCAGC              CCTCAGC(-5/-2)
BcgI           48  CGAAGACGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          271  CTAG                 C^TAG
BglII         225  AGATCT               A^GATCT
BseRI         241  GAGGAG               GAGGAG(10/8)
BsgI          690  GTGCAG               GTGCAG(16/14)
BsiHKAI       699  GTGCTC               G[AT]GC[AT]^C
BsmBI         977  CGTCTC               CGTCTC(1/5)
BsoBI         452  CCCGGG               C^[CT]CG[GA]G
BsrBI        1056  CCGCTC               CCGCTC(-3/-3)
BsrFI        1145  GCCGGC               [GA]^CCGG[CT]
BstBI          46  TTCGAA               TT^CGAA
Cfr10I       1145  GCCGGC               [GA]^CCGG[CT]
DraIII       1197  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          253  TGGCCA               [CT]^GGCC[GA]
Eam1105I      718  GACCGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         111  CCTCTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       342  CCTTGG               C^C[AT][AT]GG
MaeI          271  CTAG                 C^TAG
MaeII          53  ACGT                 A^CGT
MfeI          193  CAATTG               C^AATTG
MluNI         253  TGGCCA               TGG^CCA
MscI          253  TGGCCA               TGG^CCA
MspA1I       1138  CAGCGG               C[AC]G^C[GT]G
MunI          193  CAATTG               C^AATTG
NaeI         1145  GCCGGC               GCC^GGC
NdeI         1103  CATATG               CA^TATG
NgoMIV       1145  GCCGGC               G^CCGGC
NspV           46  TTCGAA               TT^CGAA
SfuI           46  TTCGAA               TT^CGAA
SmaI          452  CCCGGG               CCC^GGG
StyI          342  CCTTGG               C^C[AT][AT]GG
XbaI          270  TCTAGA               T^CTAGA
XcmI          389  CCAATGGCAGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         452  CCCGGG               C^CCGGG
XmaI          452  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII         610  CGCG                 CG^CG
             1154  CGCG                
AlwI         1083  GATCC                GGATC(4/5)
              325  GGATC               
BbsI           49  GAAGAC               GAAGAC(2/6)
              537  GAAGAC              
BcnI          452  CCCGG                CC^[GC]GG
              453  CCGGG               
BmyI          678  GAGCCC               G[AGT]GC[ACT]^C
              699  GTGCTC              
BpuAI          49  GAAGAC               GAAGAC(2/6)
              537  GAAGAC              
Bsp1286I      678  GAGCCC               G[AGT]GC[ACT]^C
              699  GTGCTC              
BstAPI        692  GCAGTGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1112  GCAGCGAATGC         
BstUI         610  CGCG                 CG^CG
             1154  CGCG                
BstYI         225  AGATCT               [GA]^GATC[CT]
             1082  AGATCC              
DraII         107  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1010  GGGACCC             
DsaI           59  CCGTGG               C^C[GA][CT]GG
              214  CCGTGG              
EarI           76  GAAGAG               CTCTTC(1/4)
              198  GAAGAG              
EcoO109I      107  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1010  GGGACCC             
EcoRI         746  GAATTC               G^AATTC
              830  GAATTC              
HaeIII        160  GGCC                 GG^CC
              254  GGCC                
HapII         453  CCGG                 C^CGG
             1146  CCGG                
HindIII       959  AAGCTT               A^AGCTT
             1043  AAGCTT              
HpaII         453  CCGG                 C^CGG
             1146  CCGG                
HphI          123  GGTGA                GGTGA(8/7)
              563  TCACC               
Ksp632I        76  GAAGAG               CTCTTC(1/4)
              198  GAAGAG              
MflI          225  AGATCT               [GA]^GATC[CT]
             1082  AGATCC              
MspI          453  CCGG                 C^CGG
             1146  CCGG                
MvnI          610  CGCG                 CG^CG
             1154  CGCG                
NciI          452  CCCGG                CC^[GC]GG
              453  CCGGG               
NlaIII        232  CATG                 CATG^
             1003  CATG                
PpuMI         107  GGGACCT              [GA]G^G[AT]CC[CT]
             1010  GGGACCC             
TaqI           47  TCGA                 T^CGA
              819  TCGA                
TfiI           34  GAATC                G^A[AT]TC
              294  GATTC               
TthHB8I        47  TCGA                 T^CGA
              819  TCGA                
XhoII         225  AGATCT               [GA]^GATC[CT]
             1082  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        Asp700I     
AvaII       BbvI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      FauI        Fnu4HI      
FokI        HinfI       ItaI        MaeIII      MboI        
MboII       MnlI        MslI        MvaI        MwoI        
NdeII       NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        Tsp45I      Tsp509I     
TspRI       XmnI        
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