Restriction map for pF1KB7853
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BfrI        BglII       
BlpI        Bpu1102I    BsaAI       BsaHI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsaI        RsrII       
SacII       SalI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SspBI       SspI        SwaI        Tth111I     XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Alw44I       1311  GTGCAC               G^TGCAC
ApaLI        1311  GTGCAC               G^TGCAC
AvaI         1596  CTCGAG               C^[CT]CG[GA]G
AvrII          24  CCTAGG               C^CTAGG
BanI          480  GGTGCC               G^G[CT][GA]CC
BbsI         1242  GAAGAC               GAAGAC(2/6)
BciVI         392  GTATCC               GTATCC(6/5)
BclI          617  TGATCA               T^GATCA
BglI         1201  GCCCCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI           24  CCTAGG               C^CTAGG
BpuAI        1242  GAAGAC               GAAGAC(2/6)
BsaBI         387  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1291  ACCGGA               [AT]^CCGG[AT]
BsoBI        1596  CTCGAG               C^[CT]CG[GA]G
BspHI         165  TCATGA               T^CATGA
BsrDI         381  GCAATG               GCAATG(2/0)
BssSI        1444  CACGAG               CACGAG(-5/-1)
BstAPI       1313  GCACCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI         1437  GCGC                 GCG^C
DsaI         1205  CCATGG               C^C[GA][CT]GG
FbaI          617  TGATCA               T^GATCA
HapII        1292  CCGG                 C^CGG
HhaI         1437  GCGC                 GCG^C
HinP1I       1437  GCGC                 G^CGC
HincII          7  GTTGAC               GT[CT]^[GA]AC
HindII          7  GTTGAC               GT[CT]^[GA]AC
HpaII        1292  CCGG                 C^CGG
MamI          387  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         1520  TTAA                 T^TAA
MslI          826  CATGTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1292  CCGG                 C^CGG
NcoI         1205  CCATGG               C^CATGG
NspI          927  GCATGC               [GA]CATG^[CT]
PaeR7I       1596  CTCGAG               C^TCGAG
PleI           58  GACTC                GAGTC(4/5)
PstI         1227  CTGCAG               CTGCA^G
RcaI          165  TCATGA               T^CATGA
SapI          802  GAAGAGC              GCTCTTC(1/4)
SbfI         1226  CCTGCAGG             CCTGCA^GG
SexAI         146  ACCAGGT              A^CC[AT]GGT
SphI          927  GCATGC               GCATG^C
Sse8387I     1226  CCTGCAGG             CCTGCA^GG
StuI           27  AGGCCT               AGG^CCT
TaqI         1597  TCGA                 T^CGA
Tru9I        1520  TTAA                 T^TAA
TthHB8I      1597  TCGA                 T^CGA
XcmI          147  CCAGGTGATGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1596  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Asp700I      1494  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1578  GAAAAGTTTC          
BseRI        1079  CTCCTC               GAGGAG(10/8)
             1162  GAGGAG              
BspMI         853  ACCTGC               ACCTGC(4/8)
             1225  ACCTGC              
DraIII        538  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1149  CACCATGTG           
EcoRI         504  GAATTC               G^AATTC
             1257  GAATTC              
EcoT14I        24  CCTAGG               C^C[AT][AT]GG
             1205  CCATGG              
HindIII       351  AAGCTT               A^AGCTT
              941  AAGCTT              
HphI          150  GGTGA                GGTGA(8/7)
             1275  TCACC               
MspA1I       1072  CAGCTG               C[AC]G^C[GT]G
             1587  CAGCTG              
PflMI         316  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1148  CCACCATGTGG         
PpuMI         362  GGGACCC              [GA]G^G[AT]CC[CT]
              629  AGGACCT             
PvuII        1072  CAGCTG               CAG^CTG
             1587  CAGCTG              
SacI          919  GAGCTC               GAGCT^C
             1593  GAGCTC              
SfcI          208  CTACAG               C^T[GA][CT]AG
             1227  CTGCAG              
StyI           24  CCTAGG               C^C[AT][AT]GG
             1205  CCATGG              
Tsp45I        337  GTGAC                ^GT[GC]AC
             1421  GTGAC               
Van91I        316  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1148  CCACCATGTGG         
XmnI         1494  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1578  GAAAAGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
AocI        ApoI        AspHI       AvaII       BanII       
BbvI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
TfiI        TseI        Tsp509I     TspRI       XhoII       
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