Restriction map for pF1KB7853
 (Linear) (Six-base) MAPSORT of: KIBB7853.seq  Check: 1590  from: 1  to: 1647



KIBB7853.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     862    1234    1647 
   Size:        862     372     413

  Fragments arranged by size: 

                 862     413     372
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     929    1647 
   Size:        929     718
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      49      81    1647 
   Size:         49      32    1566

  Fragments arranged by size: 

                1566      49      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      82     267     375     520    1647 
   Size:         82     185     108     145    1127

  Fragments arranged by size: 

                1127     185     145     108      82
 
ApaLI G'TGCA_C

Cuts at:      0    1311    1647 
   Size:       1311     336
 
ApoI r'AATT_y

Cuts at:      0     504     793     894    1257    1647 
   Size:        504     289     101     363     390

  Fragments arranged by size: 

                 504     390     363     289     101
 
AvaI C'yCGr_G

Cuts at:      0    1596    1647 
   Size:       1596      51
 
AvrII C'CTAG_G

Cuts at:      0      24    1647 
   Size:         24    1623
 
BanI G'GyrC_C

Cuts at:      0     480    1647 
   Size:        480    1167
 
BanII G_rGCy'C

Cuts at:      0     467     923    1203    1597    1647 
   Size:        467     456     280     394      50

  Fragments arranged by size: 

                 467     456     394     280      50
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1249    1647 
   Size:       1249     398
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     403    1647 
   Size:        403    1244
 
BclI T'GATC_A

Cuts at:      0     617    1647 
   Size:        617    1030
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1207    1647 
   Size:       1207     440
 
Bme1580I G_kGCm'C

Cuts at:      0    1315    1647 
   Size:       1315     332
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      57     717    1647 
   Size:         57     660     930

  Fragments arranged by size: 

                 930     660      57
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     117    1042    1647 
   Size:        117     925     605

  Fragments arranged by size: 

                 925     605     117
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     625     742    1565    1611    1647 
   Size:         87     538     117     823      46      36

  Fragments arranged by size: 

                 823     538     117      87      46      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     993    1067    1180    1647 
   Size:        520     473      74     113     467

  Fragments arranged by size: 

                 520     473     467     113      74
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     106     163     617     718     747    1168    1647 
   Size:        106      57     454     101      29     421     479

  Fragments arranged by size: 

                 479     454     421     106     101      57      29
 
BsaBI GATnn'nnATC

Cuts at:      0     391    1647 
   Size:        391    1256
 
BsaWI w'CCGG_w

Cuts at:      0    1291    1647 
   Size:       1291     356
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1070    1177    1647 
   Size:       1070     107     470

  Fragments arranged by size: 

                1070     470     107
 
BseYI C'CCAG_C

Cuts at:      0     590     651    1647 
   Size:        590      61     996

  Fragments arranged by size: 

                 996     590      61
 
BsiHKAI G_wGCw'C

Cuts at:      0     923    1315    1597    1647 
   Size:        923     392     282      50

  Fragments arranged by size: 

                 923     392     282      50
 
Bsp1286I G_dGCh'C

Cuts at:      0     467     923    1203    1315    1597    1647 
   Size:        467     456     280     112     282      50

  Fragments arranged by size: 

                 467     456     282     280     112      50
 
BspHI T'CATG_A

Cuts at:      0     165    1647 
   Size:        165    1482
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     862    1234    1647 
   Size:        862     372     413

  Fragments arranged by size: 

                 862     413     372
 
BsrDI GCAATG_nn'

Cuts at:      0     388    1647 
   Size:        388    1259
 
BssSI C'ACGA_G

Cuts at:      0    1444    1647 
   Size:       1444     203
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1319    1647 
   Size:       1319     328
 
BstYI r'GATC_y

Cuts at:      0     236     245     287     711     839    1618    1647 
   Size:        236       9      42     424     128     779      29

  Fragments arranged by size: 

                 779     424     236     128      42      29       9
 
Bsu36I CC'TnA_GG

Cuts at:      0     485     513     662    1031    1647 
   Size:        485      28     149     369     616

  Fragments arranged by size: 

                 616     485     369     149      28
 
BtgI C'CryG_G

Cuts at:      0    1205    1647 
   Size:       1205     442
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      23    1647 
   Size:         23    1624
 
BtsI GCAGTG_nn'

Cuts at:      0     341     643    1647 
   Size:        341     302    1004

  Fragments arranged by size: 

                1004     341     302
 
DraIII CAC_nnn'GTG

Cuts at:      0     543    1154    1647 
   Size:        543     611     493

  Fragments arranged by size: 

                 611     543     493
 
EarI CTCTTCn'nnn_

Cuts at:      0     323     797    1162    1647 
   Size:        323     474     365     485

  Fragments arranged by size: 

                 485     474     365     323
 
EcoICRI GAG'CTC

Cuts at:      0     921    1595    1647 
   Size:        921     674      52

  Fragments arranged by size: 

                 921     674      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     625     742     929    1565    1611    1647 
   Size:         87     538     117     187     636      46      36

  Fragments arranged by size: 

                 636     538     187     117      87      46      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      91    1133    1251    1647 
   Size:         91    1042     118     396

  Fragments arranged by size: 

                1042     396     118      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21     363     517     630    1647 
   Size:         21     342     154     113    1017

  Fragments arranged by size: 

                1017     342     154     113      21
 
EcoRI G'AATT_C

Cuts at:      0     504    1257    1647 
   Size:        504     753     390

  Fragments arranged by size: 

                 753     504     390
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     398     403     420     430     435     452     493     525 
   Size:        398       5      17      10       5      17      41      32

Cuts at:    525    1647 
   Size:       1122

  Fragments arranged by size: 

                1122     398      41      32      17      17      10       5
                   5
 
HincII GTy'rAC

Cuts at:      0       9    1647 
   Size:          9    1638
 
HindIII A'AGCT_T

Cuts at:      0     351     941    1647 
   Size:        351     590     706

  Fragments arranged by size: 

                 706     590     351
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     236    1647 
   Size:        236    1411
 
MslI CAynn'nnrTG

Cuts at:      0     830    1647 
   Size:        830     817
 
MspA1I CmG'CkG

Cuts at:      0    1074    1589    1647 
   Size:       1074     515      58

  Fragments arranged by size: 

                1074     515      58
 
NcoI C'CATG_G

Cuts at:      0    1205    1647 
   Size:       1205     442
 
NspI r_CATG'y

Cuts at:      0     931    1647 
   Size:        931     716
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     322    1154    1647 
   Size:        322     832     493

  Fragments arranged by size: 

                 832     493     322
 
PfoI T'CCnGG_A

Cuts at:      0     315    1647 
   Size:        315    1332
 
PpuMI rG'GwC_Cy

Cuts at:      0     363     630    1647 
   Size:        363     267    1017

  Fragments arranged by size: 

                1017     363     267
 
PsiI TTA'TAA

Cuts at:      0    1559    1647 
   Size:       1559      88
 
PspXI vC'TCGA_Gb

Cuts at:      0    1596    1647 
   Size:       1596      51
 
PstI C_TGCA'G

Cuts at:      0    1231    1647 
   Size:       1231     416
 
PvuII CAG'CTG

Cuts at:      0    1074    1589    1647 
   Size:       1074     515      58

  Fragments arranged by size: 

                1074     515      58
 
SacI G_AGCT'C

Cuts at:      0     923    1597    1647 
   Size:        923     674      50

  Fragments arranged by size: 

                 923     674      50
 
SanDI GG'GwC_CC

Cuts at:      0     363    1647 
   Size:        363    1284
 
SapI GCTCTTCn'nnn_

Cuts at:      0     797    1647 
   Size:        797     850
 
SbfI CC_TGCA'GG

Cuts at:      0    1231    1647 
   Size:       1231     416
 
SexAI A'CCwGG_T

Cuts at:      0     146    1647 
   Size:        146    1501
 
SfcI C'TryA_G

Cuts at:      0     208    1227    1647 
   Size:        208    1019     420

  Fragments arranged by size: 

                1019     420     208
 
SmlI C'TyrA_G

Cuts at:      0     499     972    1082    1159    1596    1647 
   Size:        499     473     110      77     437      51

  Fragments arranged by size: 

                 499     473     437     110      77      51
 
SphI G_CATG'C

Cuts at:      0     931    1647 
   Size:        931     716
 
StuI AGG'CCT

Cuts at:      0      29    1647 
   Size:         29    1618
 
StyI C'CwwG_G

Cuts at:      0      24    1205    1647 
   Size:         24    1181     442

  Fragments arranged by size: 

                1181     442      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     288    1647 
   Size:        288    1359
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     154    1647 
   Size:        154    1493
 
XhoI C'TCGA_G

Cuts at:      0    1596    1647 
   Size:       1596      51
 
XmnI GAAnn'nnTTC

Cuts at:      0    1498    1582    1647 
   Size:       1498      84      65

  Fragments arranged by size: 

                1498      84      65
 

 Enzymes that do cut:

     AarI     AcuI     AloI    AlwNI    ApaLI     ApoI     AvaI    AvrII
     BanI    BanII     BbsI    BciVI     BclI     BglI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaWI    BseRI    BseYI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BssSI   BstAPI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    Hin4I   HincII  HindIII     MmeI     MslI
   MspA1I     NcoI     NspI    PflMI     PfoI    PpuMI     PsiI    PspXI
     PstI    PvuII     SacI    SanDI     SapI     SbfI    SexAI     SfcI
     SmlI     SphI     StuI     StyI    TaqII     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI     AscI     AseI    AsiSI     BaeI
    BamHI     BbeI    BbvCI     BcgI    BfrBI    BglII     BlpI    BmgBI
     BmtI   Bpu10I    BsaAI    BsaHI    BsaXI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BstBI
   BstEII    BstXI  BstZ17I     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EciI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI     PsrI     PvuI
    RsrII    SacII     SalI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI     TstI
  Tth111I     XbaI     XmaI     ZraI
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