Restriction map for pF1KB7633
 (Linear) (Six-base) MAPSORT of: KIBB7633.seq  Check: 6525  from: 1  to: 963



KIBB7633.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     568     717     963 
   Size:        232     336     149     246

  Fragments arranged by size: 

                 336     246     232     149
 
AflII C'TTAA_G

Cuts at:      0     415     667     963 
   Size:        415     252     296

  Fragments arranged by size: 

                 415     296     252
 
AflIII A'CryG_T

Cuts at:      0     591     843     963 
   Size:        591     252     120

  Fragments arranged by size: 

                 591     252     120
 
AleI CACnn'nnGTG

Cuts at:      0     776     963 
   Size:        776     187
 
AlwNI CAG_nnn'CTG

Cuts at:      0     383     635     963 
   Size:        383     252     328

  Fragments arranged by size: 

                 383     328     252
 
ApaLI G'TGCA_C

Cuts at:      0     505     757     963 
   Size:        505     252     206

  Fragments arranged by size: 

                 505     252     206
 
ApoI r'AATT_y

Cuts at:      0     230     822     963 
   Size:        230     592     141

  Fragments arranged by size: 

                 592     230     141
 
BanII G_rGCy'C

Cuts at:      0     493     963 
   Size:        493     470
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     220     401     963 
   Size:        220     181     562

  Fragments arranged by size: 

                 562     220     181
 
BclI T'GATC_A

Cuts at:      0     462     963 
   Size:        462     501
 
BglII A'GATC_T

Cuts at:      0     430     963 
   Size:        430     533
 
Bme1580I G_kGCm'C

Cuts at:      0     509     761     963 
   Size:        509     252     202

  Fragments arranged by size: 

                 509     252     202
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     264     963 
   Size:        264     699
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     377     461     545     629     713     881     963 
   Size:        151     226      84      84      84      84     168      82

  Fragments arranged by size: 

                 226     168     151      84      84      84      84      82
            
 
BsaAI yAC'GTr

Cuts at:      0     846     963 
   Size:        846     117
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     963 
   Size:         91     872
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     963 
   Size:        269     694
 
BsiHKAI G_wGCw'C

Cuts at:      0     493     509     761     963 
   Size:        493      16     252     202

  Fragments arranged by size: 

                 493     252     202      16
 
BsmI GAATG_Cn'

Cuts at:      0     379     631     963 
   Size:        379     252     332

  Fragments arranged by size: 

                 379     332     252
 
Bsp1286I G_dGCh'C

Cuts at:      0     493     509     761     963 
   Size:        493      16     252     202

  Fragments arranged by size: 

                 493     252     202      16
 
BsrDI GCAATG_nn'

Cuts at:      0      85     963 
   Size:         85     878
 
BstYI r'GATC_y

Cuts at:      0     430     963 
   Size:        430     533
 
Bsu36I CC'TnA_GG

Cuts at:      0     835     963 
   Size:        835     128
 
BtsI GCAGTG_nn'

Cuts at:      0      65     384     636     963 
   Size:         65     319     252     327

  Fragments arranged by size: 

                 327     319     252      65
 
ClaI AT'CG_AT

Cuts at:      0     653     963 
   Size:        653     310
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      24     963 
   Size:         24     939
 
EaeI y'GGCC_r

Cuts at:      0     945     963 
   Size:        945      18
 
EcoICRI GAG'CTC

Cuts at:      0     491     963 
   Size:        491     472
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     232     377     461     545     568     629     713 
   Size:        151      81     145      84      84      23      61      84

Cuts at:    713     717     881     963 
   Size:          4     164      82

  Fragments arranged by size: 

                 164     151     145      84      84      84      82      81
                  61      23       4
 
EcoRI G'AATT_C

Cuts at:      0     822     963 
   Size:        822     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     480     512     646     678     963 
   Size:        480      32     134      32     285

  Fragments arranged by size: 

                 480     285     134      32      32
 
MfeI C'AATT_G

Cuts at:      0     284     963 
   Size:        284     679
 
MscI TGG'CCA

Cuts at:      0     947     963 
   Size:        947      16
 
MslI CAynn'nnrTG

Cuts at:      0     500     776     963 
   Size:        500     276     187

  Fragments arranged by size: 

                 500     276     187
 
MspA1I CmG'CkG

Cuts at:      0     210     963 
   Size:        210     753
 
NspI r_CATG'y

Cuts at:      0     595     963 
   Size:        595     368
 
PciI A'CATG_T

Cuts at:      0     591     963 
   Size:        591     372
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      54     963 
   Size:         54     909
 
PfoI T'CCnGG_A

Cuts at:      0      47     963 
   Size:         47     916
 
PmlI CAC'GTG

Cuts at:      0     846     963 
   Size:        846     117
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168     963 
   Size:        136      32     795

  Fragments arranged by size: 

                 795     136      32
 
PsiI TTA'TAA

Cuts at:      0     875     922     963 
   Size:        875      47      41

  Fragments arranged by size: 

                 875      47      41
 
PstI C_TGCA'G

Cuts at:      0       9     175     963 
   Size:          9     166     788

  Fragments arranged by size: 

                 788     166       9
 
PvuII CAG'CTG

Cuts at:      0     210     963 
   Size:        210     753
 
SacI G_AGCT'C

Cuts at:      0     493     963 
   Size:        493     470
 
SexAI A'CCwGG_T

Cuts at:      0      22     963 
   Size:         22     941
 
SfcI C'TryA_G

Cuts at:      0       5     171     963 
   Size:          5     166     792

  Fragments arranged by size: 

                 792     166       5
 
SmlI C'TyrA_G

Cuts at:      0     415     667     963 
   Size:        415     252     296

  Fragments arranged by size: 

                 415     296     252
 
StuI AGG'CCT

Cuts at:      0     814     963 
   Size:        814     149
 
StyI C'CwwG_G

Cuts at:      0     191     963 
   Size:        191     772
 
Tth111I GACn'n_nGTC

Cuts at:      0     179     963 
   Size:        179     784
 
XmnI GAAnn'nnTTC

Cuts at:      0     394     562     963 
   Size:        394     168     401

  Fragments arranged by size: 

                 401     394     168
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI    AlwNI    ApaLI     ApoI    BanII
     BbsI     BclI    BglII Bme1580I     BmrI     BpmI    BsaAI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BsrDI    BstYI   Bsu36I     BtsI
     ClaI     DrdI     EaeI  EcoICRI  Eco57MI    EcoRI    Hin4I     MfeI
     MscI     MslI   MspA1I     NspI     PciI    PflMI     PfoI     PmlI
     PpiI     PsiI     PstI    PvuII     SacI    SexAI     SfcI     SmlI
     StuI     StyI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI    CspCI
     DraI   DraIII     EagI     EarI     EciI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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