Restriction map for pF1KB7701
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BalI        BamHI       BanII       BbrPI       BbsI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrDI       BsrGI       Bst1107I    
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco47III    Eco81I      
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        HpaI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NcoI        NdeI        NheI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          832  GAATTT               [GA]^AATT[CT]
AcyI          589  GGCGCC               G[GA]^CG[CT]C
AflIII       1116  ACATGT               A^C[GA][CT]GT
AlwNI         259  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          832  GAATTT               [GA]^AATT[CT]
AviII        1109  TGCGCA               TGC^GCA
BbeI          589  GGCGCC               GGCGC^C
BbvCI         555  CCTCAGC              CCTCAGC(-5/-2)
BcgI          281  CGACCCAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          839  CTAG                 C^TAG
BglI          568  GCCGGCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         390  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         589  GGCGCC               G[GA]^CG[CT]C
BsaWI        1132  ACCGGA               [AT]^CCGG[AT]
BsmFI         962  GTCCC                GGGAC(10/14)
BspLU11I     1116  ACATGT               A^CATGT
BspMI         624  GCAGGT               ACCTGC(4/8)
BssHII        363  GCGCGC               G^CGCGC
BssSI          70  CACGAG               CACGAG(-5/-1)
BstAPI        829  GCAGAATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1069  TTCGAA               TT^CGAA
BstYI         813  AGATCC               [GA]^GATC[CT]
DraII        1141  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        663  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I     1141  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FspI         1109  TGCGCA               TGC^GCA
HaeII         589  GGCGCC               [GA]GCGC^[CT]
Hin1I         589  GGCGCC               G[GA]^CG[CT]C
HincII       1188  GTCAAC               GT[CT]^[GA]AC
HindII       1188  GTCAAC               GT[CT]^[GA]AC
HindIII       995  AAGCTT               A^AGCTT
KasI          589  GGCGCC               G^GCGCC
MaeI          839  CTAG                 C^TAG
MamI          390  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          813  AGATCC               [GA]^GATC[CT]
NarI          589  GGCGCC               GG^CGCC
NotI          506  GCGGCCGC             GC^GGCCGC
NspV         1069  TTCGAA               TT^CGAA
PstI          256  CTGCAG               CTGCA^G
SapI          534  GAAGAGC              GCTCTTC(1/4)
SfcI          256  CTGCAG               C^T[GA][CT]AG
SfoI          589  GGCGCC               GGC^GCC
SfuI         1069  TTCGAA               TT^CGAA
SmaI          597  CCCGGG               CCC^GGG
XhoII         813  AGATCC               [GA]^GATC[CT]
XmaCI         597  CCCGGG               C^CCGGG
XmaI          597  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Alw44I        289  GTGCAC               G^TGCAC
             1062  GTGCAC              
ApaLI         289  GTGCAC               G^TGCAC
             1062  GTGCAC              
AspHI         289  GTGCAC               G[AT]GC[AT]^C
             1062  GTGCAC              
AvaI            6  CTCGGG               C^[CT]CG[GA]G
              597  CCCGGG              
AvaII         671  GGTCC                G^G[AT]CC
              961  GGTCC               
BanI          402  GGTGCC               G^G[CT][GA]CC
              589  GGCGCC              
BsiHKAI       289  GTGCAC               G[AT]GC[AT]^C
             1062  GTGCAC              
BsoBI           6  CTCGGG               C^[CT]CG[GA]G
              597  CCCGGG              
BsrBI         474  GAGCGG               CCGCTC(-3/-3)
              537  GAGCGG              
BstF5I         90  CATCC                GGATG(2/0)
              396  CATCC               
EarI          534  GAAGAG               CTCTTC(1/4)
              546  GAAGAG              
EcoNI         781  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              895  CCTCATAAAGG         
FokI           90  CATCC                GGATG(9/13)
              396  CATCC               
HgaI          321  GACGC                GACGC(5/10)
              512  GCGTC               
Ksp632I       534  GAAGAG               CTCTTC(1/4)
              546  GAAGAG              
MaeII         974  ACGT                 A^CGT
             1066  ACGT                
MseI          860  TTAA                 T^TAA
             1213  TTAA                
MspA1I        262  CCGCTG               C[AC]G^C[GT]G
              558  CAGCGG              
NspI         1116  ACATGT               [GA]CATG^[CT]
             1218  ACATGC              
PleI          349  GACTC                GAGTC(4/5)
              968  GAGTC               
SfaNI          45  GATGC                GCATC(5/9)
              871  GATGC               
TfiI          390  GATTC                G^A[AT]TC
             1124  GAATC               
Tru9I         860  TTAA                 T^TAA
             1213  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BbvI        
BcnI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrFI       BsrI        BssKI       
BstEII      BstNI       BstPI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DsaI        EaeI        EagI        EclXI       
Eco52I      EcoO65I     EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MslI        MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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