Restriction map for pF1KE0340
 (Linear) (Six-base) MAPSORT of: KIEE0340.seq  Check: 6928  from: 1  to: 1521



KIEE0340.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0     536    1521 
   Size:        536     985
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     391    1521 
   Size:         82     309    1130

  Fragments arranged by size: 

                1130     309      82
 
AflIII A'CryG_T

Cuts at:      0    1030    1521 
   Size:       1030     491
 
AleI CACnn'nnGTG

Cuts at:      0    1035    1521 
   Size:       1035     486
 
AlwNI CAG_nnn'CTG

Cuts at:      0     663     714    1521 
   Size:        663      51     807

  Fragments arranged by size: 

                 807     663      51
 
ApoI r'AATT_y

Cuts at:      0     559     859    1521 
   Size:        559     300     662

  Fragments arranged by size: 

                 662     559     300
 
AvaI C'yCGr_G

Cuts at:      0    1276    1521 
   Size:       1276     245
 
BanII G_rGCy'C

Cuts at:      0     876    1355    1521 
   Size:        876     479     166

  Fragments arranged by size: 

                 876     479     166
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     998    1521 
   Size:        998     523
 
BclI T'GATC_A

Cuts at:      0     734    1521 
   Size:        734     787
 
BglI GCCn_nnn'nGGC

Cuts at:      0     419    1234    1521 
   Size:        419     815     287

  Fragments arranged by size: 

                 815     419     287
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     467    1521 
   Size:        467    1054
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     551    1283    1521 
   Size:        176     375     732     238

  Fragments arranged by size: 

                 732     375     238     176
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245    1521 
   Size:        245    1276
 
BsaAI yAC'GTr

Cuts at:      0    1033    1037    1521 
   Size:       1033       4     484

  Fragments arranged by size: 

                1033     484       4
 
BsaBI GATnn'nnATC

Cuts at:      0    1339    1521 
   Size:       1339     182
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     710     740    1521 
   Size:        710      30     781

  Fragments arranged by size: 

                 781     710      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160    1521 
   Size:        160    1361
 
BseYI C'CCAG_C

Cuts at:      0     145     772    1521 
   Size:        145     627     749

  Fragments arranged by size: 

                 749     627     145
 
BsiHKAI G_wGCw'C

Cuts at:      0     299    1521 
   Size:        299    1222
 
BsiWI C'GTAC_G

Cuts at:      0    1033    1521 
   Size:       1033     488
 
BsmI GAATG_Cn'

Cuts at:      0     395     767     871    1521 
   Size:        395     372     104     650

  Fragments arranged by size: 

                 650     395     372     104
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     586    1521 
   Size:        586     935
 
Bsp1286I G_dGCh'C

Cuts at:      0     299     876    1355    1521 
   Size:        299     577     479     166

  Fragments arranged by size: 

                 577     479     299     166
 
BspHI T'CATG_A

Cuts at:      0    1202    1521 
   Size:       1202     319
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1043    1521 
   Size:       1043     478
 
BssSI C'ACGA_G

Cuts at:      0     875    1521 
   Size:        875     646
 
BstYI r'GATC_y

Cuts at:      0     468    1097    1521 
   Size:        468     629     424

  Fragments arranged by size: 

                 629     468     424
 
Bsu36I CC'TnA_GG

Cuts at:      0    1067    1521 
   Size:       1067     454
 
BtgI C'CryG_G

Cuts at:      0      51    1521 
   Size:         51    1470
 
DraIII CAC_nnn'GTG

Cuts at:      0     945    1521 
   Size:        945     576
 
EaeI y'GGCC_r

Cuts at:      0    1039    1266    1521 
   Size:       1039     227     255

  Fragments arranged by size: 

                1039     255     227
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     176     391     551    1283    1521 
   Size:         82      94     215     160     732     238

  Fragments arranged by size: 

                 732     238     215     160      94      82
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     667    1521 
   Size:        667     854
 
EcoO109I rG'GnC_Cy

Cuts at:      0     623    1521 
   Size:        623     898
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     466     859    1521 
   Size:        466     393     662

  Fragments arranged by size: 

                 662     466     393
 
HaeII r_GCGC'y

Cuts at:      0     726    1521 
   Size:        726     795
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     726     758    1521 
   Size:        726      32     763

  Fragments arranged by size: 

                 763     726      32
 
HindIII A'AGCT_T

Cuts at:      0    1137    1183    1521 
   Size:       1137      46     338

  Fragments arranged by size: 

                1137     338      46
 
MfeI C'AATT_G

Cuts at:      0    1346    1521 
   Size:       1346     175
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     326    1521 
   Size:        326    1195
 
MscI TGG'CCA

Cuts at:      0    1041    1268    1521 
   Size:       1041     227     253

  Fragments arranged by size: 

                1041     253     227
 
MslI CAynn'nnrTG

Cuts at:      0    1035    1521 
   Size:       1035     486
 
MspA1I CmG'CkG

Cuts at:      0      37      53     714    1521 
   Size:         37      16     661     807

  Fragments arranged by size: 

                 807     661      37      16
 
NspI r_CATG'y

Cuts at:      0     634    1308    1521 
   Size:        634     674     213

  Fragments arranged by size: 

                 674     634     213
 
PasI CC'CwG_GG

Cuts at:      0    1129    1521 
   Size:       1129     392
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     288    1048    1521 
   Size:        288     760     473

  Fragments arranged by size: 

                 760     473     288
 
PfoI T'CCnGG_A

Cuts at:      0     192    1521 
   Size:        192    1329
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      60      92    1521 
   Size:         60      32    1429

  Fragments arranged by size: 

                1429      60      32
 
PstI C_TGCA'G

Cuts at:      0     750    1328    1470    1521 
   Size:        750     578     142      51

  Fragments arranged by size: 

                 750     578     142      51
 
PvuII CAG'CTG

Cuts at:      0     714    1521 
   Size:        714     807
 
SacII CC_GC'GG

Cuts at:      0      54    1521 
   Size:         54    1467
 
SfcI C'TryA_G

Cuts at:      0      38     746    1152    1324    1466    1521 
   Size:         38     708     406     172     142      55

  Fragments arranged by size: 

                 708     406     172     142      55      38
 
SmlI C'TyrA_G

Cuts at:      0     260    1521 
   Size:        260    1261
 
StyI C'CwwG_G

Cuts at:      0       4    1521 
   Size:          4    1517
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     176     914    1521 
   Size:        176     738     607

  Fragments arranged by size: 

                 738     607     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1442    1474    1521 
   Size:       1442      32      47

  Fragments arranged by size: 

                1442      47      32
 
XbaI T'CTAG_A

Cuts at:      0    1092    1521 
   Size:       1092     429
 
XmnI GAAnn'nnTTC

Cuts at:      0     560     839    1088    1521 
   Size:        560     279     249     433

  Fragments arranged by size: 

                 560     433     279     249
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    AlwNI     ApoI     AvaI    BanII
     BbsI     BclI     BglI     BmrI     BpmI    BpuEI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BssSI    BstYI   Bsu36I     BtgI   DraIII     EaeI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I  HindIII     MfeI
     MmeI     MscI     MslI   MspA1I     NspI     PasI    PflMI     PfoI
     PsrI     PstI    PvuII    SacII     SfcI     SmlI     StyI    TaqII
     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI
    BsaHI    BsaWI     BsgI    BsiEI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XcmI     XhoI     XmaI
     ZraI
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