Restriction map for pF1KB6409
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspEI       BspHI       BspLU11I    BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII           6  CGCG                 CG^CG
AcsI          172  AAATTT               [GA]^AATT[CT]
AfaI          822  GTAC                 GT^AC
Alw44I        650  GTGCAC               G^TGCAC
AlwNI         491  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         650  GTGCAC               G^TGCAC
ApoI          172  AAATTT               [GA]^AATT[CT]
BanII         812  GAGCTC               G[GA]GC[CT]^C
BbsI          799  GAAGAC               GAAGAC(2/6)
BclI          341  TGATCA               T^GATCA
BcnI          646  CCGGG                CC^[GC]GG
BfaI          809  CTAG                 C^TAG
BpuAI         799  GAAGAC               GAAGAC(2/6)
BsaI           89  GAGACC               GGTCTC(1/5)
BseRI         482  CTCCTC               GAGGAG(10/8)
BspDI         145  ATCGAT               AT^CGAT
BspMI         738  ACCTGC               ACCTGC(4/8)
BsrBI          25  CCGCTC               CCGCTC(-3/-3)
BsrDI         124  GCAATG               GCAATG(2/0)
BstNI         774  CCAGG                CC^[AT]GG
BstUI           6  CGCG                 CG^CG
CfoI            7  GCGC                 GCG^C
Cfr13I        493  GGCCC                G^G[ACGT]CC
ClaI          145  ATCGAT               AT^CGAT
DraI          465  TTTAAA               TTT^AAA
DraII         492  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EcoNI         484  CCTCAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      492  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        774  CCAGG                ^CC[AT]GG
FbaI          341  TGATCA               T^GATCA
HapII         646  CCGG                 C^CGG
HhaI            7  GCGC                 GCG^C
HinP1I          7  GCGC                 G^CGC
HpaII         646  CCGG                 C^CGG
HphI          223  GGTGA                GGTGA(8/7)
MaeI          809  CTAG                 C^TAG
MaeIII        900  GTTAC                ^GT[ACGT]AC
MspA1I         16  CCGCTG               C[AC]G^C[GT]G
MspI          646  CCGG                 C^CGG
MvaI          774  CCAGG                CC^[AT]GG
MvnI            6  CGCG                 CG^CG
NciI          646  CCGGG                CC^[GC]GG
NspI          608  ACATGC               [GA]CATG^[CT]
PleI          480  GACTC                GAGTC(4/5)
RsaI          822  GTAC                 GT^AC
SacI          812  GAGCTC               GAGCT^C
Sau96I        493  GGCCC                G^G[ACGT]CC
ScaI          821  AGTACT               AGT^ACT
TaqI          146  TCGA                 T^CGA
TthHB8I       146  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          748  GATCC                GGATC(4/5)
              192  GGATC               
AspHI         650  GTGCAC               G[AT]GC[AT]^C
              812  GAGCTC              
BbvI          547  GCAGC                GCAGC(8/12)
               18  GCTGC               
BpmI          659  CTCCAG               CTGGAG(16/14)
              542  CTGGAG              
BsaJI         773  CCCAGG               C^C[ACGT][ACGT]GG
              774  CCAGGG              
BsiHKAI       650  GTGCAC               G[AT]GC[AT]^C
              812  GAGCTC              
BsiYI          93  CCCTGTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              484  CCTCAAGCAGG         
BslI           93  CCCTGTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              484  CCTCAAGCAGG         
BsmAI          89  GAGAC                GTCTC(1/5)
              478  GAGAC               
BsrI          133  ACTGG                ACTGG(1/-1)
              628  ACTGG               
BssKI         646  CCGGG                ^CC[ACGT]GG
              774  CCAGG               
BstF5I        453  CATCC                GGATG(2/0)
              780  GGATG               
FokI          453  CATCC                GGATG(9/13)
              780  GGATG               
HaeIII          3  GGCC                 GG^CC
              493  GGCC                
MseI          187  TTAA                 T^TAA
              466  TTAA                
ScrFI         646  CCGGG                CC^[ACGT]GG
              774  CCAGG               
SfaNI         538  GATGC                GCATC(5/9)
               54  GCATC               
TfiI          639  GATTC                G^A[AT]TC
              879  GAATC               
Tru9I         187  TTAA                 T^TAA
              466  TTAA                
TseI           18  GCTGC                G^C[AT]GC
              547  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AluI        BmyI        Bsp1286I    Cac8I       
DdeI        DpnII       FauI        Fnu4HI      HinfI       
ItaI        MboI        MboII       MnlI        MwoI        
NdeII       NlaIII      Sau3AI      SmlI        Tsp509I     
TspRI       
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