Restriction map for pF1KE0149
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        Eam1105I    EarI        Eco47III    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MunI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccII         148  CGCG                 CG^CG
AocI          193  CCTGAGG              CC^T[ACGT]AGG
AspHI         247  GAGCTC               G[AT]GC[AT]^C
AvaI           21  CTCGGG               C^[CT]CG[GA]G
BalI          113  TGGCCA               TGG^CCA
BbsI           99  GAAGAC               GAAGAC(2/6)
BbvCI         253  CCTCAGC              CCTCAGC(-5/-2)
BcnI          220  CCCGG                CC^[GC]GG
BpmI          328  CTGGAG               CTGGAG(16/14)
BpuAI          99  GAAGAC               GAAGAC(2/6)
BsiEI         179  CGGCCG               CG[GA][CT]^CG
BsiHKAI       247  GAGCTC               G[AT]GC[AT]^C
BsmAI         144  GAGAC                GTCTC(1/5)
BsmBI         144  GAGACG               CGTCTC(1/5)
BsoBI          21  CTCGGG               C^[CT]CG[GA]G
BsrBI         204  GAGCGG               CCGCTC(-3/-3)
BssSI         259  CACGAG               CACGAG(-5/-1)
BstAPI        258  GCACGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         148  CGCG                 CG^CG
Bsu36I        193  CCTGAGG              CC^T[ACGT]AGG
DsaI           41  CCACGG               C^C[GA][CT]GG
EagI          179  CGGCCG               C^GGCCG
EclXI         179  CGGCCG               C^GGCCG
Eco52I        179  CGGCCG               C^GGCCG
Eco81I        193  CCTGAGG              CC^T[ACGT]AGG
EcoNI         309  CCTGAGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          146  GACGC                GACGC(5/10)
MluNI         113  TGGCCA               TGG^CCA
MscI          113  TGGCCA               TGG^CCA
MspA1I        212  CAGCGG               C[AC]G^C[GT]G
MvnI          148  CGCG                 CG^CG
NciI          220  CCCGG                CC^[GC]GG
SacI          247  GAGCTC               GAGCT^C


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AvaII          25  GGACC                G^G[AT]CC
              293  GGACC               
BanI          189  GGCACC               G^G[CT][GA]CC
              216  GGCACC              
BanII          78  GAGCCC               G[GA]GC[CT]^C
              247  GAGCTC              
BbvI          163  GCTGC                GCAGC(8/12)
              264  GCTGC               
BglI           40  GCCACGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              181  GCCGTGACGGC         
BmyI           78  GAGCCC               G[AGT]GC[ACT]^C
              247  GAGCTC              
BseRI          66  CTCCTC               GAGGAG(10/8)
              274  GAGGAG              
BsiYI         309  CCTGAGTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              384  CCAGGTGGAGG         
BslI          309  CCTGAGTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              384  CCAGGTGGAGG         
BsmFI          24  GGGAC                GGGAC(10/14)
              292  GGGAC               
Bsp1286I       78  GAGCCC               G[AGT]GC[ACT]^C
              247  GAGCTC              
BsrI           83  CCAGT                ACTGG(1/-1)
              280  CCAGT               
BstNI         243  CCAGG                CC^[AT]GG
              384  CCAGG               
DraII          24  GGGACCT              [GA]G^G[ACGT]CC[CT]
              292  GGGACCC             
EcoO109I       24  GGGACCT              [GA]G^G[ACGT]CC[CT]
              292  GGGACCC             
EcoRII        243  CCAGG                ^CC[AT]GG
              384  CCAGG               
EcoT14I        35  CCAAGG               C^C[AT][AT]GG
              107  CCAAGG              
FauI          149  GCGGG                CCCGC(4/6)
              206  GCGGG               
MaeIII         86  GTGAC                ^GT[ACGT]AC
              184  GTGAC               
MvaI          243  CCAGG                CC^[AT]GG
              384  CCAGG               
PpuMI          24  GGGACCT              [GA]G^G[AT]CC[CT]
              292  GGGACCC             
StyI           35  CCAAGG               C^C[AT][AT]GG
              107  CCAAGG              
TseI          163  GCTGC                G^C[AT]GC
              264  GCTGC               
Tsp45I         86  GTGAC                ^GT[GC]AC
              184  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AluI        BsaJI       BssKI       Cac8I       
Cfr13I      DdeI        EaeI        Fnu4HI      HaeIII      
HapII       HinfI       HpaII       ItaI        MboII       
MnlI        MspI        MwoI        NlaIV       PleI        
Sau96I      ScrFI       TspRI       
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