Restriction map for pF1KB7314
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspI        AvaI        BbrPI       
BcgI        BciVI       BfrI        BglI        BlpI        
Bpu1102I    BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsoBI       BspDI       BspEI       
BspLU11I    BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRV       FauI        FseI        HindIII     HpaI        
KpnI        KspI        MamI        MluI        MroI        
NaeI        NgoMIV      NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    StuI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII          85  CGCG                 CG^CG
AclI         1136  AACGTT               AA^CGTT
AcyI          187  GGCGCC               G[GA]^CG[CT]C
AhdI          535  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         2157  ATTAAT               AT^TAAT
AsnI         2157  ATTAAT               AT^TAAT
Asp700I       694  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         535  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII          10  TGCGCA               TGC^GCA
AvrII        1062  CCTAGG               C^CTAGG
BamHI        2877  GGATCC               G^GATCC
BbeI          187  GGCGCC               GGCGC^C
BbsI          198  GAAGAC               GAAGAC(2/6)
BclI         2274  TGATCA               T^GATCA
BcnI         3233  CCCGG                CC^[GC]GG
BlnI         1062  CCTAGG               C^CTAGG
BpuAI         198  GAAGAC               GAAGAC(2/6)
BsaAI        2750  TACGTG               [CT]AC^GT[GA]
BsaHI         187  GGCGCC               G[GA]^CG[CT]C
BseRI          97  GAGGAG               GAGGAG(10/8)
BsmFI        2082  GGGAC                GGGAC(10/14)
BspHI         177  TCATGA               T^CATGA
BsrBI        1763  CCGCTC               CCGCTC(-3/-3)
BstAPI        325  GCATTATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          85  CGCG                 CG^CG
DraII        1803  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1475  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      535  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1803  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1412  GAATTC               G^AATTC
FbaI         2274  TGATCA               T^GATCA
FspI           10  TGCGCA               TGC^GCA
HapII        3234  CCGG                 C^CGG
HgaI         1502  GCGTC                GACGC(5/10)
Hin1I         187  GGCGCC               G[GA]^CG[CT]C
HincII        466  GTTGAC               GT[CT]^[GA]AC
HindII        466  GTTGAC               GT[CT]^[GA]AC
HpaII        3234  CCGG                 C^CGG
KasI          187  GGCGCC               G^GCGCC
MspI         3234  CCGG                 C^CGG
MvnI           85  CGCG                 CG^CG
NarI          187  GGCGCC               GG^CGCC
NciI         3233  CCCGG                CC^[GC]GG
NheI         2458  GCTAGC               G^CTAGC
PpuMI        1803  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         148  GACAGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2157  ATTAAT               AT^TAAT
Psp1406I     1136  AACGTT               AA^CGTT
RcaI          177  TCATGA               T^CATGA
SapI         1732  GAAGAGC              GCTCTTC(1/4)
SfoI          187  GGCGCC               GGC^GCC
SmlI         1094  CTCAAG               C^T[CT][GA]AG
SspI         2245  AATATT               AAT^ATT
SwaI         1310  ATTTAAAT             ATTT^AAAT
XcmI         2204  CCAATGGCAATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          694  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          406  GTCTAC               GT^[AC][GT]AC
             2443  GTAGAC              
AlwNI        2114  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2848  CAGTTGCTG           
AocI         1961  CCTCAGG              CC^T[ACGT]AGG
             3170  CCTCAGG             
BsgI          704  CTGCAC               GTGCAG(16/14)
              941  GTGCAG              
BsmBI         154  CGTCTC               CGTCTC(1/5)
             1503  CGTCTC              
BsmI          375  GAATGC               GAATGC(1/-1)
             2990  GAATGC              
Bsp1407I     1023  TGTACA               T^GTACA
             2719  TGTACA              
BsrDI        2210  GCAATG               GCAATG(2/0)
             2994  GCAATG              
BsrGI        1023  TGTACA               T^GTACA
             2719  TGTACA              
BstXI        2798  CCAAAACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3198  CCAAAGTGCTGG        
Bsu36I       1961  CCTCAGG              CC^T[ACGT]AGG
             3170  CCTCAGG             
DraI          549  TTTAAA               TTT^AAA
             1311  TTTAAA              
Eco81I       1961  CCTCAGG              CC^T[ACGT]AGG
             3170  CCTCAGG             
EcoT22I       422  ATGCAT               ATGCA^T
              745  ATGCAT              
HaeII         132  GGCGCT               [GA]GCGC^[CT]
              187  GGCGCC              
MfeI         1027  CAATTG               C^AATTG
             2933  CAATTG              
MunI         1027  CAATTG               C^AATTG
             2933  CAATTG              
NdeI          671  CATATG               CA^TATG
             2827  CATATG              
NsiI          422  ATGCAT               ATGCA^T
              745  ATGCAT              
NspI         1041  GCATGC               [GA]CATG^[CT]
             2536  GCATGC              
PvuII        1333  CAGCTG               CAG^CTG
             2255  CAGCTG              
SacI         2430  GAGCTC               GAGCT^C
             2765  GAGCTC              
SphI         1041  GCATGC               GCATG^C
             2536  GCATGC              
SspBI        1023  TGTACA               T^GTACA
             2719  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BalI        BanI        
BanII       BbvCI       BbvI        BfaI        BglII       
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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