Restriction map for pF1KB7314
 (Linear) (Six-base) MAPSORT of: KIBB7314.seq  Check: 8118  from: 1  to: 3237



KIBB7314.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3095    3237 
   Size:       3095     142
 
AccI GT'mk_AC

Cuts at:      0     407    2444    3237 
   Size:        407    2037     793

  Fragments arranged by size: 

                2037     793     407
 
AclI AA'CG_TT

Cuts at:      0    1137    3237 
   Size:       1137    2100
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272    2285    3237 
   Size:        272    2013     952

  Fragments arranged by size: 

                2013     952     272
 
AhdI GACnn_n'nnGTC

Cuts at:      0     540    3237 
   Size:        540    2697
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2119    2853    3237 
   Size:       2119     734     384

  Fragments arranged by size: 

                2119     734     384
 
ApoI r'AATT_y

Cuts at:      0     494     608    1174    1412    1595    2151    3237 
   Size:        494     114     566     238     183     556    1086

  Fragments arranged by size: 

                1086     566     556     494     238     183     114
 
AseI AT'TA_AT

Cuts at:      0    2158    3237 
   Size:       2158    1079
 
AvrII C'CTAG_G

Cuts at:      0    1062    3237 
   Size:       1062    2175
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1478    1511    3237 
   Size:       1478      33    1726

  Fragments arranged by size: 

                1726    1478      33
 
BamHI G'GATC_C

Cuts at:      0    2877    3237 
   Size:       2877     360
 
BanI G'GyrC_C

Cuts at:      0     187    2025    3083    3237 
   Size:        187    1838    1058     154

  Fragments arranged by size: 

                1838    1058     187     154
 
BanII G_rGCy'C

Cuts at:      0     515    2434    2769    3237 
   Size:        515    1919     335     468

  Fragments arranged by size: 

                1919     515     468     335
 
BbeI G_GCGC'C

Cuts at:      0     191    3237 
   Size:        191    3046
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     205    3237 
   Size:        205    3032
 
BbvCI CC'TCA_GC

Cuts at:      0    2174    3055    3189    3237 
   Size:       2174     881     134      48

  Fragments arranged by size: 

                2174     881     134      48
 
BclI T'GATC_A

Cuts at:      0    2274    3237 
   Size:       2274     963
 
BfrBI ATG'CAT

Cuts at:      0     424     747    3237 
   Size:        424     323    2490

  Fragments arranged by size: 

                2490     424     323
 
BglII A'GATC_T

Cuts at:      0    2467    2480    2778    2920    3237 
   Size:       2467      13     298     142     317

  Fragments arranged by size: 

                2467     317     298     142      13
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1839    1844    2507    2883    3237 
   Size:       1839       5     663     376     354

  Fragments arranged by size: 

                1839     663     376     354       5
 
BmtI G_CTAG'C

Cuts at:      0    2462    3237 
   Size:       2462     775
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1535    2069    2783    3237 
   Size:       1535     534     714     454

  Fragments arranged by size: 

                1535     714     534     454
 
Bpu10I CC'TnA_GC

Cuts at:      0    1394    1766    2174    3055    3189    3237 
   Size:       1394     372     408     881     134      48

  Fragments arranged by size: 

                1394     881     408     372     134      48
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1079    3237 
   Size:       1079    2158
 
BsaAI yAC'GTr

Cuts at:      0    2752    3237 
   Size:       2752     485
 
BsaHI Gr'CG_yC

Cuts at:      0     188    3237 
   Size:        188    3049
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2310    2340    3237 
   Size:       2310      30     897

  Fragments arranged by size: 

                2310     897      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112    3237 
   Size:        112    3125
 
BseYI C'CCAG_C

Cuts at:      0     472    2253    2415    3205    3237 
   Size:        472    1781     162     790      32

  Fragments arranged by size: 

                1781     790     472     162      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     689     962    3237 
   Size:        689     273    2275

  Fragments arranged by size: 

                2275     689     273
 
BsiHKAI G_wGCw'C

Cuts at:      0    1485    2434    2769    3237 
   Size:       1485     949     335     468

  Fragments arranged by size: 

                1485     949     468     335
 
BsmI GAATG_Cn'

Cuts at:      0     381    2996    3237 
   Size:        381    2615     241

  Fragments arranged by size: 

                2615     381     241
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     160    1509    3237 
   Size:        160    1349    1728

  Fragments arranged by size: 

                1728    1349     160
 
Bsp1286I G_dGCh'C

Cuts at:      0     515    1485    2434    2769    3237 
   Size:        515     970     949     335     468

  Fragments arranged by size: 

                 970     949     515     468     335
 
BspHI T'CATG_A

Cuts at:      0     177    3237 
   Size:        177    3060
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1682    2456    3095    3237 
   Size:       1682     774     639     142

  Fragments arranged by size: 

                1682     774     639     142
 
BsrBI CCG'CTC

Cuts at:      0    1765    3237 
   Size:       1765    1472
 
BsrDI GCAATG_nn'

Cuts at:      0    2217    3001    3237 
   Size:       2217     784     236

  Fragments arranged by size: 

                2217     784     236
 
BsrGI T'GTAC_A

Cuts at:      0    1023    2719    3237 
   Size:       1023    1696     518

  Fragments arranged by size: 

                1696    1023     518
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     331    3237 
   Size:        331    2906
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2805    3205    3237 
   Size:       2805     400      32

  Fragments arranged by size: 

                2805     400      32
 
BstYI r'GATC_y

Cuts at:      0     759     828    2017    2467    2480    2778    2877    2920 
   Size:        759      69    1189     450      13     298      99      43

Cuts at:   2920    3237 
   Size:        317

  Fragments arranged by size: 

                1189     759     450     317     298      99      69      43
                  13
 
Bsu36I CC'TnA_GG

Cuts at:      0    1962    3171    3237 
   Size:       1962    1209      66

  Fragments arranged by size: 

                1962    1209      66
 
BtgI C'CryG_G

Cuts at:      0    1035    1738    2136    3237 
   Size:       1035     703     398    1101

  Fragments arranged by size: 

                1101    1035     703     398
 
BtsI GCAGTG_nn'

Cuts at:      0      20    1441    3013    3237 
   Size:         20    1421    1572     224

  Fragments arranged by size: 

                1572    1421     224      20
 
DraI TTT'AAA

Cuts at:      0     551    1313    3237 
   Size:        551     762    1924

  Fragments arranged by size: 

                1924     762     551
 
DraIII CAC_nnn'GTG

Cuts at:      0    1480    3237 
   Size:       1480    1757
 
EaeI y'GGCC_r

Cuts at:      0     840    1209    3144    3237 
   Size:        840     369    1935      93

  Fragments arranged by size: 

                1935     840     369      93
 
EarI CTCTTCn'nnn_

Cuts at:      0      89     163    1727    2295    2304    3237 
   Size:         89      74    1564     568       9     933

  Fragments arranged by size: 

                1564     933     568      89      74       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      82    3014    3237 
   Size:         82    2932     223

  Fragments arranged by size: 

                2932     223      82
 
EcoICRI GAG'CTC

Cuts at:      0    2432    2767    3237 
   Size:       2432     335     470

  Fragments arranged by size: 

                2432     470     335
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272    1535    2069    2285    2783    3237 
   Size:        272    1263     534     216     498     454

  Fragments arranged by size: 

                1263     534     498     454     272     216
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1804    3237 
   Size:       1804    1433
 
EcoRI G'AATT_C

Cuts at:      0    1412    3237 
   Size:       1412    1825
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     475    3237 
   Size:        475    2762
 
FspI TGC'GCA

Cuts at:      0      12    3237 
   Size:         12    3225
 
HaeII r_GCGC'y

Cuts at:      0     136     191    3237 
   Size:        136      55    3046

  Fragments arranged by size: 

                3046     136      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     497     526     529     558     636     668    2995    3027 
   Size:        497      29       3      29      78      32    2327      32

Cuts at:   3027    3237 
   Size:        210

  Fragments arranged by size: 

                2327     497     210      78      32      32      29      29
                   3
 
HincII GTy'rAC

Cuts at:      0     468    3237 
   Size:        468    2769
 
KasI G'GCGC_C

Cuts at:      0     187    3237 
   Size:        187    3050
 
MfeI C'AATT_G

Cuts at:      0    1027    2933    3237 
   Size:       1027    1906     304

  Fragments arranged by size: 

                1906    1027     304
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     721    3237 
   Size:        287     434    2516

  Fragments arranged by size: 

                2516     434     287
 
MscI TGG'CCA

Cuts at:      0     842    1211    3146    3237 
   Size:        842     369    1935      91

  Fragments arranged by size: 

                1935     842     369      91
 
MslI CAynn'nnrTG

Cuts at:      0     182     632    1040    3237 
   Size:        182     450     408    2197

  Fragments arranged by size: 

                2197     450     408     182
 
MspA1I CmG'CkG

Cuts at:      0      34    1335    2257    3237 
   Size:         34    1301     922     980

  Fragments arranged by size: 

                1301     980     922      34
 
NarI GG'CG_CC

Cuts at:      0     188    3237 
   Size:        188    3049
 
NcoI C'CATG_G

Cuts at:      0    1035    1738    2136    3237 
   Size:       1035     703     398    1101

  Fragments arranged by size: 

                1101    1035     703     398
 
NdeI CA'TA_TG

Cuts at:      0     672    2828    3237 
   Size:        672    2156     409

  Fragments arranged by size: 

                2156     672     409
 
NheI G'CTAG_C

Cuts at:      0    2458    3237 
   Size:       2458     779
 
NsiI A_TGCA'T

Cuts at:      0     426     749    3237 
   Size:        426     323    2488

  Fragments arranged by size: 

                2488     426     323
 
NspI r_CATG'y

Cuts at:      0    1045    2540    3237 
   Size:       1045    1495     697

  Fragments arranged by size: 

                1495    1045     697
 
PfoI T'CCnGG_A

Cuts at:      0    2969    3237 
   Size:       2969     268
 
PpuMI rG'GwC_Cy

Cuts at:      0    1804    3237 
   Size:       1804    1433
 
PshAI GACnn'nnGTC

Cuts at:      0     152    3237 
   Size:        152    3085
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     520     552    3237 
   Size:        520      32    2685

  Fragments arranged by size: 

                2685     520      32
 
PstI C_TGCA'G

Cuts at:      0    1569    1679    3020    3237 
   Size:       1569     110    1341     217

  Fragments arranged by size: 

                1569    1341     217     110
 
PvuII CAG'CTG

Cuts at:      0    1335    2257    3237 
   Size:       1335     922     980

  Fragments arranged by size: 

                1335     980     922
 
SacI G_AGCT'C

Cuts at:      0    2434    2769    3237 
   Size:       2434     335     468

  Fragments arranged by size: 

                2434     468     335
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1727    3237 
   Size:       1727    1510
 
SfcI C'TryA_G

Cuts at:      0    1180    1565    1675    3016    3237 
   Size:       1180     385     110    1341     221

  Fragments arranged by size: 

                1341    1180     385     221     110
 
SfoI GGC'GCC

Cuts at:      0     189    3237 
   Size:        189    3048
 
SmlI C'TyrA_G

Cuts at:      0    1094    3237 
   Size:       1094    2143
 
SphI G_CATG'C

Cuts at:      0    1045    2540    3237 
   Size:       1045    1495     697

  Fragments arranged by size: 

                1495    1045     697
 
SspI AAT'ATT

Cuts at:      0    2247    3237 
   Size:       2247     990
 
StyI C'CwwG_G

Cuts at:      0     396     488    1035    1062    1738    2021    2136    3237 
   Size:        396      92     547      27     676     283     115    1101

  Fragments arranged by size: 

                1101     676     547     396     283     115      92      27
            
 
SwaI ATTT'AAAT

Cuts at:      0    1313    3237 
   Size:       1313    1924
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2267    2670    3237 
   Size:       2267     403     567

  Fragments arranged by size: 

                2267     567     403
 
TatI w'GTAC_w

Cuts at:      0     589    1023    1233    1606    2719    3237 
   Size:        589     434     210     373    1113     518

  Fragments arranged by size: 

                1113     589     518     434     373     210
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3201    3233    3237 
   Size:       3201      32       4

  Fragments arranged by size: 

                3201      32       4
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2211    3237 
   Size:       2211    1026
 
XmnI GAAnn'nnTTC

Cuts at:      0     698    3237 
   Size:        698    2539
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AhdI    AlwNI     ApoI     AseI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BclI    BfrBI    BglII     BmrI     BmtI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     MfeI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NsiI     NspI     PfoI
    PpuMI    PshAI     PsrI     PstI    PvuII     SacI     SapI     SfcI
     SfoI     SmlI     SphI     SspI     StyI     SwaI    TaqII     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI     BcgI    BciVI
     BglI     BlpI Bme1580I    BmgBI     BplI     BsaI    BsaBI    BsaWI
    BsiEI    BsiWI    BspEI    BsrFI   BssHII    BssSI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI     DrdI     EagI    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     KpnI     MluI     NaeI   NgoMIV
     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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