Restriction map for pF1KA1594
 (Linear) (Six-base) MAPSORT of: KIAA1594.seq  Check: 6878  from: 1  to: 2937



KIAA1594.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:24  ..

AclI AA'CG_TT

Cuts at:      0    1971    2937 
   Size:       1971     966
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31    2416    2633    2693    2937 
   Size:         31    2385     217      60     244

  Fragments arranged by size: 

                2385     244     217      60      31
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2924    2937 
   Size:       2924      13
 
AleI CACnn'nnGTG

Cuts at:      0    2908    2937 
   Size:       2908      29
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1298    1431    2937 
   Size:       1298     133    1506

  Fragments arranged by size: 

                1506    1298     133
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1017    2442    2611    2937 
   Size:       1017    1425     169     326

  Fragments arranged by size: 

                1425    1017     326     169
 
ApoI r'AATT_y

Cuts at:      0      32     137    1133    1291    1380    1651    1866    1903 
   Size:         32     105     996     158      89     271     215      37

Cuts at:   1903    2937 
   Size:       1034

  Fragments arranged by size: 

                1034     996     271     215     158     105      89      37
                  32
 
AvrII C'CTAG_G

Cuts at:      0    1666    2937 
   Size:       1666    1271
 
BamHI G'GATC_C

Cuts at:      0      73     823    2298    2937 
   Size:         73     750    1475     639

  Fragments arranged by size: 

                1475     750     639      73
 
BanII G_rGCy'C

Cuts at:      0    1927    2937 
   Size:       1927    1010
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1360    2036    2512    2923    2937 
   Size:       1360     676     476     411      14

  Fragments arranged by size: 

                1360     676     476     411      14
 
BbvCI CC'TCA_GC

Cuts at:      0     917    1984    2618    2937 
   Size:        917    1067     634     319

  Fragments arranged by size: 

                1067     917     634     319
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2794    2828    2937 
   Size:       2794      34     109

  Fragments arranged by size: 

                2794     109      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1129    2937 
   Size:       1129    1808
 
BfrBI ATG'CAT

Cuts at:      0    2551    2937 
   Size:       2551     386
 
BglII A'GATC_T

Cuts at:      0    2855    2937 
   Size:       2855      82
 
Bme1580I G_kGCm'C

Cuts at:      0     992    2937 
   Size:        992    1945
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      59     185    2937 
   Size:         59     126    2752

  Fragments arranged by size: 

                2752     126      59
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1278    2937 
   Size:       1278    1659
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     690    1069    1391    1621    2342    2374    2794 
   Size:        152     538     379     322     230     721      32     420

Cuts at:   2794    2937 
   Size:        143

  Fragments arranged by size: 

                 721     538     420     379     322     230     152     143
                  32
 
Bpu10I CC'TnA_GC

Cuts at:      0     917    1984    2618    2937 
   Size:        917    1067     634     319

  Fragments arranged by size: 

                1067     917     634     319
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1204    2937 
   Size:       1204    1733
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1195    2937 
   Size:       1195    1742
 
BsaBI GATnn'nnATC

Cuts at:      0    2816    2937 
   Size:       2816     121
 
BsaWI w'CCGG_w

Cuts at:      0    2212    2937 
   Size:       2212     725
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1541    2937 
   Size:       1541    1396
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2824    2937 
   Size:       2824     113
 
BsiHKAI G_wGCw'C

Cuts at:      0    1638    1927    2937 
   Size:       1638     289    1010

  Fragments arranged by size: 

                1638    1010     289
 
BsmI GAATG_Cn'

Cuts at:      0    1062    2937 
   Size:       1062    1875
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     344    2937 
   Size:        344    2593
 
Bsp1286I G_dGCh'C

Cuts at:      0     992    1638    1927    2937 
   Size:        992     646     289    1010

  Fragments arranged by size: 

                1010     992     646     289
 
BspHI T'CATG_A

Cuts at:      0    1287    2175    2937 
   Size:       1287     888     762

  Fragments arranged by size: 

                1287     888     762
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1054    2937 
   Size:       1054    1883
 
BsrDI GCAATG_nn'

Cuts at:      0    1776    2937 
   Size:       1776    1161
 
BsrFI r'CCGG_y

Cuts at:      0    2666    2937 
   Size:       2666     271
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1017    2937 
   Size:       1017    1920
 
BstBI TT'CG_AA

Cuts at:      0      36     477    2937 
   Size:         36     441    2460

  Fragments arranged by size: 

                2460     441      36
 
BstYI r'GATC_y

Cuts at:      0      73     699     823    2298    2365    2855    2937 
   Size:         73     626     124    1475      67     490      82

  Fragments arranged by size: 

                1475     626     490     124      82      73      67
 
Bsu36I CC'TnA_GG

Cuts at:      0     659    2937 
   Size:        659    2278
 
BtgI C'CryG_G

Cuts at:      0    1123    2937 
   Size:       1123    1814
 
ClaI AT'CG_AT

Cuts at:      0     508    2937 
   Size:        508    2429
 
EarI CTCTTCn'nnn_

Cuts at:      0     755     843    2111    2414    2937 
   Size:        755      88    1268     303     523

  Fragments arranged by size: 

                1268     755     523     303      88
 
EcoICRI GAG'CTC

Cuts at:      0    1925    2937 
   Size:       1925    1012
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     152     690    1069    1391    1621    2342    2374 
   Size:         31     121     538     379     322     230     721      32

Cuts at:   2374    2416    2633    2693    2794    2937 
   Size:         42     217      60     101     143

  Fragments arranged by size: 

                 721     538     379     322     230     217     143     121
                 101      60      42      32      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0     380    1671    2937 
   Size:        380    1291    1266

  Fragments arranged by size: 

                1291    1266     380
 
EcoRI G'AATT_C

Cuts at:      0      32     137    1866    2937 
   Size:         32     105    1729    1071

  Fragments arranged by size: 

                1729    1071     105      32
 
EcoRV GAT'ATC

Cuts at:      0     626    1188    2937 
   Size:        626     562    1749

  Fragments arranged by size: 

                1749     626     562
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1588    1944    2137    2874    2937 
   Size:       1588     356     193     737      63

  Fragments arranged by size: 

                1588     737     356     193      63
 
FspI TGC'GCA

Cuts at:      0    1823    2937 
   Size:       1823    1114
 
FspAI rTGC'GCAy

Cuts at:      0    1823    2937 
   Size:       1823    1114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       6      38     661     693    1469    1501    2554    2586 
   Size:          6      32     623      32     776      32    1053      32

Cuts at:   2586    2634    2666    2910    2937 
   Size:         48      32     244      27

  Fragments arranged by size: 

                1053     776     623     244      48      32      32      32
                  32      32      27       6
 
HincII GTy'rAC

Cuts at:      0     221    2937 
   Size:        221    2716
 
HpaI GTT'AAC

Cuts at:      0     221    2937 
   Size:        221    2716
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1795    2937 
   Size:       1795    1142
 
MslI CAynn'nnrTG

Cuts at:      0    2180    2908    2937 
   Size:       2180     728      29

  Fragments arranged by size: 

                2180     728      29
 
MspA1I CmG'CkG

Cuts at:      0     741    1317    2131    2937 
   Size:        741     576     814     806

  Fragments arranged by size: 

                 814     806     741     576
 
NcoI C'CATG_G

Cuts at:      0    1123    2937 
   Size:       1123    1814
 
NdeI CA'TA_TG

Cuts at:      0    1826    2937 
   Size:       1826    1111
 
NsiI A_TGCA'T

Cuts at:      0    2553    2937 
   Size:       2553     384
 
NspI r_CATG'y

Cuts at:      0    1475    2937 
   Size:       1475    1462
 
PpuMI rG'GwC_Cy

Cuts at:      0     380    1671    2937 
   Size:        380    1291    1266

  Fragments arranged by size: 

                1291    1266     380
 
PstI C_TGCA'G

Cuts at:      0     327    1022    2937 
   Size:        327     695    1915

  Fragments arranged by size: 

                1915     695     327
 
PvuII CAG'CTG

Cuts at:      0     741    1317    2131    2937 
   Size:        741     576     814     806

  Fragments arranged by size: 

                 814     806     741     576
 
SacI G_AGCT'C

Cuts at:      0    1927    2937 
   Size:       1927    1010
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2111    2414    2937 
   Size:       2111     303     523

  Fragments arranged by size: 

                2111     523     303
 
SfcI C'TryA_G

Cuts at:      0     323     518    1018    1247    2937 
   Size:        323     195     500     229    1690

  Fragments arranged by size: 

                1690     500     323     229     195
 
SmlI C'TyrA_G

Cuts at:      0    1219    2937 
   Size:       1219    1718
 
StuI AGG'CCT

Cuts at:      0    2932    2937 
   Size:       2932       5
 
StyI C'CwwG_G

Cuts at:      0     142     827    1123    1666    1809    1928    2937 
   Size:        142     685     296     543     143     119    1009

  Fragments arranged by size: 

                1009     685     543     296     143     142     119
 
TatI w'GTAC_w

Cuts at:      0    2600    2937 
   Size:       2600     337
 
Tth111I GACn'n_nGTC

Cuts at:      0    1767    2937 
   Size:       1767    1170
 
XbaI T'CTAG_A

Cuts at:      0     294    1837    2937 
   Size:        294    1543    1100

  Fragments arranged by size: 

                1543    1100     294
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     900     964    1926    2937 
   Size:        900      64     962    1011

  Fragments arranged by size: 

                1011     962     900      64
 
XmnI GAAnn'nnTTC

Cuts at:      0    2120    2937 
   Size:       2120     817
 

 Enzymes that do cut:

     AclI     AcuI     AhdI     AleI     AlfI    AlwNI     ApoI    AvrII
    BamHI    BanII     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaWI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI   BstAPI    BstBI    BstYI   Bsu36I     BtgI
     ClaI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    FspAI    Hin4I   HincII     HpaI     MmeI     MslI   MspA1I
     NcoI     NdeI     NsiI     NspI    PpuMI     PstI    PvuII     SacI
     SapI     SfcI     SmlI     StuI     StyI     TatI  Tth111I     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI
     BanI     BbeI     BclI     BglI     BlpI    BmgBI     BmtI    BsaAI
    BsaHI    BsaXI    BseYI    BsiEI    BsiWI    BspEI    BsrBI    BsrGI
   BssHII    BssSI   BstEII    BstXI  BstZ17I    BtgZI     BtsI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI     FseI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TstI     XhoI
     XmaI     ZraI
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