Restriction map for pF1KE0551
 (Linear) (Six-base) MAPSORT of: KIEE0551.seq  Check: 1001  from: 1  to: 861



KIEE0551.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AccI GT'mk_AC

Cuts at:      0     182     200     861 
   Size:        182      18     661

  Fragments arranged by size: 

                 661     182      18
 
AclI AA'CG_TT

Cuts at:      0     262     861 
   Size:        262     599
 
AflIII A'CryG_T

Cuts at:      0     593     797     861 
   Size:        593     204      64

  Fragments arranged by size: 

                 593     204      64
 
AleI CACnn'nnGTG

Cuts at:      0     802     861 
   Size:        802      59
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     352     861 
   Size:        352     509
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     472     504     861 
   Size:        472      32     357

  Fragments arranged by size: 

                 472     357      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     384     540     861 
   Size:        384     156     321

  Fragments arranged by size: 

                 384     321     156
 
ApaI G_GGCC'C

Cuts at:      0      18     861 
   Size:         18     843
 
BamHI G'GATC_C

Cuts at:      0     432     861 
   Size:        432     429
 
BanI G'GyrC_C

Cuts at:      0     169     409     682     861 
   Size:        169     240     273     179

  Fragments arranged by size: 

                 273     240     179     169
 
BanII G_rGCy'C

Cuts at:      0      18     259     649     861 
   Size:         18     241     390     212

  Fragments arranged by size: 

                 390     241     212      18
 
BbeI G_GCGC'C

Cuts at:      0     686     861 
   Size:        686     175
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     782     861 
   Size:        782      79
 
BbvCI CC'TCA_GC

Cuts at:      0     694     833     861 
   Size:        694     139      28

  Fragments arranged by size: 

                 694     139      28
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     449     483     861 
   Size:        449      34     378

  Fragments arranged by size: 

                 449     378      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      22     606     861 
   Size:         22     584     255

  Fragments arranged by size: 

                 584     255      22
 
Bme1580I G_kGCm'C

Cuts at:      0      18     861 
   Size:         18     843
 
BmgBI CAC'GTC

Cuts at:      0     800     861 
   Size:        800      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     262     861 
   Size:        262     599
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838     861 
   Size:        838      23
 
Bpu10I CC'TnA_GC

Cuts at:      0     694     833     861 
   Size:        694     139      28

  Fragments arranged by size: 

                 694     139      28
 
BsaAI yAC'GTr

Cuts at:      0     596     861 
   Size:        596     265
 
BsaBI GATnn'nnATC

Cuts at:      0     628     861 
   Size:        628     233
 
BsaHI Gr'CG_yC

Cuts at:      0     683     861 
   Size:        683     178
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     276     306     472     502     861 
   Size:        276      30     166      30     359

  Fragments arranged by size: 

                 359     276     166      30      30
 
BseYI C'CCAG_C

Cuts at:      0     340     774     861 
   Size:        340     434      87

  Fragments arranged by size: 

                 434     340      87
 
BsiEI CG_ry'CG

Cuts at:      0     590     861 
   Size:        590     271
 
BsmI GAATG_Cn'

Cuts at:      0     225     393     861 
   Size:        225     168     468

  Fragments arranged by size: 

                 468     225     168
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     259     649     861 
   Size:         18     241     390     212

  Fragments arranged by size: 

                 390     241     212      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      68     344     430     861 
   Size:         68     276      86     431

  Fragments arranged by size: 

                 431     276      86      68
 
BsrBI CCG'CTC

Cuts at:      0       9     614     861 
   Size:          9     605     247

  Fragments arranged by size: 

                 605     247       9
 
BsrDI GCAATG_nn'

Cuts at:      0     686     861 
   Size:        686     175
 
BsrFI r'CCGG_y

Cuts at:      0     605     861 
   Size:        605     256
 
BssSI C'ACGA_G

Cuts at:      0     175     700     861 
   Size:        175     525     161

  Fragments arranged by size: 

                 525     175     161
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     522     774     861 
   Size:        522     252      87

  Fragments arranged by size: 

                 522     252      87
 
BstYI r'GATC_y

Cuts at:      0     380     432     861 
   Size:        380      52     429

  Fragments arranged by size: 

                 429     380      52
 
BtgI C'CryG_G

Cuts at:      0     476     515     861 
   Size:        476      39     346

  Fragments arranged by size: 

                 476     346      39
 
BtsI GCAGTG_nn'

Cuts at:      0     336     861 
   Size:        336     525
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     543     861 
   Size:        543     318
 
EaeI y'GGCC_r

Cuts at:      0      74     598     861 
   Size:         74     524     263

  Fragments arranged by size: 

                 524     263      74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     624     861 
   Size:        624     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838     861 
   Size:        838      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     159     292     419     447     779     861 
   Size:         15     144     133     127      28     332      82

  Fragments arranged by size: 

                 332     144     133     127      82      28      15
 
HaeII r_GCGC'y

Cuts at:      0     686     861 
   Size:        686     175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     276     308     608     640     861 
   Size:        276      32     300      32     221

  Fragments arranged by size: 

                 300     276     221      32      32
 
HincII GTy'rAC

Cuts at:      0     201     861 
   Size:        201     660
 
KasI G'GCGC_C

Cuts at:      0     682     861 
   Size:        682     179
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     861 
   Size:        527     334
 
MscI TGG'CCA

Cuts at:      0      76     600     861 
   Size:         76     524     261

  Fragments arranged by size: 

                 524     261      76
 
MslI CAynn'nnrTG

Cuts at:      0     802     861 
   Size:        802      59
 
NaeI GCC'GGC

Cuts at:      0     607     861 
   Size:        607     254
 
NarI GG'CG_CC

Cuts at:      0     683     861 
   Size:        683     178
 
NgoMIV G'CCGG_C

Cuts at:      0     605     861 
   Size:        605     256
 
NspI r_CATG'y

Cuts at:      0     144     861 
   Size:        144     717
 
PfoI T'CCnGG_A

Cuts at:      0     214     854     861 
   Size:        214     640       7

  Fragments arranged by size: 

                 640     214       7
 
PmlI CAC'GTG

Cuts at:      0     596     861 
   Size:        596     265
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     472     504     861 
   Size:        472      32     357

  Fragments arranged by size: 

                 472     357      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     292     419     861 
   Size:        292     127     442

  Fragments arranged by size: 

                 442     292     127
 
PspOMI G'GGCC_C

Cuts at:      0      14     861 
   Size:         14     847
 
PstI C_TGCA'G

Cuts at:      0      65     331     861 
   Size:         65     266     530

  Fragments arranged by size: 

                 530     266      65
 
PvuI CG_AT'CG

Cuts at:      0     590     861 
   Size:        590     271
 
SalI G'TCGA_C

Cuts at:      0     199     861 
   Size:        199     662
 
SexAI A'CCwGG_T

Cuts at:      0     395     861 
   Size:        395     466
 
SfcI C'TryA_G

Cuts at:      0      61     327     861 
   Size:         61     266     534

  Fragments arranged by size: 

                 534     266      61
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      22     861 
   Size:         22     839
 
SfoI GGC'GCC

Cuts at:      0     684     861 
   Size:        684     177
 
StuI AGG'CCT

Cuts at:      0     373     861 
   Size:        373     488
 
StyI C'CwwG_G

Cuts at:      0     122     368     861 
   Size:        122     246     493

  Fragments arranged by size: 

                 493     246     122
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     187     397     861 
   Size:        187     210     464

  Fragments arranged by size: 

                 464     210     187
 
TatI w'GTAC_w

Cuts at:      0     482     861 
   Size:        482     379
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     249     861 
   Size:        120     129     612

  Fragments arranged by size: 

                 612     129     120
 

 Enzymes that do cut:

     AccI     AclI   AflIII     AleI     AlfI     AloI    AlwNI     ApaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI    BsiEI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BssSI    BstXI    BstYI     BtgI     BtsI     DrdI     EaeI
     EciI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI     NaeI     NarI   NgoMIV     NspI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI     PvuI     SalI    SexAI     SfcI
     SfiI     SfoI     StuI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII     AgeI     AhdI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BplI    BpuEI
     BsaI    BsaWI    BseRI     BsgI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EarI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI   MspA1I     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
    PshAI     PsiI    PspXI     PsrI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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