Restriction map for pF1KB7352
 (Linear) (Six-base) MAPSORT of: KIBB7352.seq  Check: 9220  from: 1  to: 2529



KIBB7352.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2364    2529 
   Size:       2364     165
 
AccI GT'mk_AC

Cuts at:      0     935    2529 
   Size:        935    1594
 
Acc65I G'GTAC_C

Cuts at:      0    1199    2529 
   Size:       1199    1330
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     649    1297    1609    1932    2129    2529 
   Size:        190     459     648     312     323     197     400

  Fragments arranged by size: 

                 648     459     400     323     312     197     190
 
AfeI AGC'GCT

Cuts at:      0      46    2529 
   Size:         46    2483
 
AflII C'TTAA_G

Cuts at:      0     271    2529 
   Size:        271    2258
 
AleI CACnn'nnGTG

Cuts at:      0    1055    2287    2529 
   Size:       1055    1232     242

  Fragments arranged by size: 

                1232    1055     242
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1679    2060    2529 
   Size:       1679     381     469

  Fragments arranged by size: 

                1679     469     381
 
AlwNI CAG_nnn'CTG

Cuts at:      0     168    2013    2307    2529 
   Size:        168    1845     294     222

  Fragments arranged by size: 

                1845     294     222     168
 
ApaI G_GGCC'C

Cuts at:      0    1521    2062    2529 
   Size:       1521     541     467

  Fragments arranged by size: 

                1521     541     467
 
ApoI r'AATT_y

Cuts at:      0     280    1186    1743    2529 
   Size:        280     906     557     786

  Fragments arranged by size: 

                 906     786     557     280
 
AvaI C'yCGr_G

Cuts at:      0     142     203     362    1957    2529 
   Size:        142      61     159    1595     572

  Fragments arranged by size: 

                1595     572     159     142      61
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     538     571    2529 
   Size:        538      33    1958

  Fragments arranged by size: 

                1958     538      33
 
BamHI G'GATC_C

Cuts at:      0      74     849    2529 
   Size:         74     775    1680

  Fragments arranged by size: 

                1680     775      74
 
BanI G'GyrC_C

Cuts at:      0    1199    2529 
   Size:       1199    1330
 
BanII G_rGCy'C

Cuts at:      0      86     675     930    1330    1433    1521    2062    2225 
   Size:         86     589     255     400     103      88     541     163

Cuts at:   2225    2529 
   Size:        304

  Fragments arranged by size: 

                 589     541     400     304     255     163     103      88
                  86
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1034    1279    2529 
   Size:       1034     245    1250

  Fragments arranged by size: 

                1250    1034     245
 
BclI T'GATC_A

Cuts at:      0     302    2529 
   Size:        302    2227
 
BglI GCCn_nnn'nGGC

Cuts at:      0     408    2529 
   Size:        408    2121
 
BglII A'GATC_T

Cuts at:      0     800    2090    2529 
   Size:        800    1290     439

  Fragments arranged by size: 

                1290     800     439
 
BlpI GC'TnA_GC

Cuts at:      0    1033    2219    2529 
   Size:       1033    1186     310

  Fragments arranged by size: 

                1186    1033     310
 
Bme1580I G_kGCm'C

Cuts at:      0    1521    2049    2062    2504    2529 
   Size:       1521     528      13     442      25

  Fragments arranged by size: 

                1521     528     442      25      13
 
BmgBI CAC'GTC

Cuts at:      0    1879    2529 
   Size:       1879     650
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1859    2529 
   Size:       1859     670
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     751    1983    2529 
   Size:        751    1232     546

  Fragments arranged by size: 

                1232     751     546
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     178     865    1566    2500    2529 
   Size:         49     129     687     701     934      29

  Fragments arranged by size: 

                 934     701     687     129      49      29
 
Bpu10I CC'TnA_GC

Cuts at:      0      80     608    2051    2268    2429    2529 
   Size:         80     528    1443     217     161     100

  Fragments arranged by size: 

                1443     528     217     161     100      80
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     947    1980    2529 
   Size:        947    1033     549

  Fragments arranged by size: 

                1033     947     549
 
BsaAI yAC'GTr

Cuts at:      0    1436    2289    2529 
   Size:       1436     853     240

  Fragments arranged by size: 

                1436     853     240
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     915     945    2529 
   Size:        915      30    1584

  Fragments arranged by size: 

                1584     915      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     725    1294    2529 
   Size:        130     595     569    1235

  Fragments arranged by size: 

                1235     595     569     130
 
BseYI C'CCAG_C

Cuts at:      0     218    2409    2529 
   Size:        218    2191     120

  Fragments arranged by size: 

                2191     218     120
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1318    1639    2050    2392    2529 
   Size:       1318     321     411     342     137

  Fragments arranged by size: 

                1318     411     342     321     137
 
BsiHKAI G_wGCw'C

Cuts at:      0      86    2225    2528    2529 
   Size:         86    2139     303       1

  Fragments arranged by size: 

                2139     303      86       1
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      25    2036    2529 
   Size:         25    2011     493

  Fragments arranged by size: 

                2011     493      25
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     675     930    1330    1433    1521    2049    2062 
   Size:         86     589     255     400     103      88     528      13

Cuts at:   2062    2225    2504    2528    2529 
   Size:        163     279      24       1

  Fragments arranged by size: 

                 589     528     400     279     255     163     103      88
                  86      24      13       1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     776    2364    2529 
   Size:        776    1588     165

  Fragments arranged by size: 

                1588     776     165
 
BsrBI CCG'CTC

Cuts at:      0      53      72    2529 
   Size:         53      19    2457

  Fragments arranged by size: 

                2457      53      19
 
BsrDI GCAATG_nn'

Cuts at:      0    1109    1904    2529 
   Size:       1109     795     625

  Fragments arranged by size: 

                1109     795     625
 
BssSI C'ACGA_G

Cuts at:      0    2392    2529 
   Size:       2392     137
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1827    2529 
   Size:       1827     702
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     792    2529 
   Size:        792    1737
 
BstYI r'GATC_y

Cuts at:      0      74     800     849    1589    2090    2354    2529 
   Size:         74     726      49     740     501     264     175

  Fragments arranged by size: 

                 740     726     501     264     175      74      49
 
Bsu36I CC'TnA_GG

Cuts at:      0     184    2529 
   Size:        184    2345
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      96     528    2529 
   Size:         96     432    2001

  Fragments arranged by size: 

                2001     432      96
 
BtsI GCAGTG_nn'

Cuts at:      0    1804    2049    2529 
   Size:       1804     245     480

  Fragments arranged by size: 

                1804     480     245
 
EaeI y'GGCC_r

Cuts at:      0     101     308    1013    2208    2529 
   Size:        101     207     705    1195     321

  Fragments arranged by size: 

                1195     705     321     207     101
 
EarI CTCTTCn'nnn_

Cuts at:      0    1119    1340    1628    2179    2255    2287    2529 
   Size:       1119     221     288     551      76      32     242

  Fragments arranged by size: 

                1119     551     288     242     221      76      32
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1261    1603    2362    2529 
   Size:       1261     342     759     167

  Fragments arranged by size: 

                1261     759     342     167
 
EcoICRI GAG'CTC

Cuts at:      0      84    2223    2529 
   Size:         84    2139     306

  Fragments arranged by size: 

                2139     306      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     178     190     649     865    1297    1566    1609 
   Size:         49     129      12     459     216     432     269      43

Cuts at:   1609    1932    2129    2500    2529 
   Size:        323     197     371      29

  Fragments arranged by size: 

                 459     432     371     323     269     216     197     129
                  49      43      29      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     187    2529 
   Size:        187    2342
 
EcoO109I rG'GnC_Cy

Cuts at:      0     208    1482    1517    2058    2418    2529 
   Size:        208    1274      35     541     360     111

  Fragments arranged by size: 

                1274     541     360     208     111      35
 
EcoRI G'AATT_C

Cuts at:      0     280    1186    2529 
   Size:        280     906    1343

  Fragments arranged by size: 

                1343     906     280
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1194    2529 
   Size:       1194    1335
 
HaeII r_GCGC'y

Cuts at:      0      48    1501    2529 
   Size:         48    1453    1028

  Fragments arranged by size: 

                1453    1028      48
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     662     694     917     949    1129    1161    1378    1410 
   Size:        662      32     223      32     180      32     217      32

Cuts at:   1410    1633    1665    2529 
   Size:        223      32     864

  Fragments arranged by size: 

                 864     662     223     223     217     180      32      32
                  32      32      32
 
KpnI G_GTAC'C

Cuts at:      0    1203    2529 
   Size:       1203    1326
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     696    1322    1997    2529 
   Size:        696     626     675     532

  Fragments arranged by size: 

                 696     675     626     532
 
MscI TGG'CCA

Cuts at:      0     310    1015    2529 
   Size:        310     705    1514

  Fragments arranged by size: 

                1514     705     310
 
MslI CAynn'nnrTG

Cuts at:      0    1055    1694    1717    2287    2529 
   Size:       1055     639      23     570     242

  Fragments arranged by size: 

                1055     639     570     242      23
 
MspA1I CmG'CkG

Cuts at:      0      96     222     394     792    2064    2107    2529 
   Size:         96     126     172     398    1272      43     422

  Fragments arranged by size: 

                1272     422     398     172     126      96      43
 
NspI r_CATG'y

Cuts at:      0    1827    2205    2301    2529 
   Size:       1827     378      96     228

  Fragments arranged by size: 

                1827     378     228      96
 
PfoI T'CCnGG_A

Cuts at:      0    2360    2529 
   Size:       2360     169
 
PmlI CAC'GTG

Cuts at:      0    2289    2529 
   Size:       2289     240
 
PpuMI rG'GwC_Cy

Cuts at:      0    1482    2418    2529 
   Size:       1482     936     111

  Fragments arranged by size: 

                1482     936     111
 
PshAI GACnn'nnGTC

Cuts at:      0     229     394    2529 
   Size:        229     165    2135

  Fragments arranged by size: 

                2135     229     165
 
PsiI TTA'TAA

Cuts at:      0     338    2529 
   Size:        338    2191
 
PspOMI G'GGCC_C

Cuts at:      0    1517    2058    2529 
   Size:       1517     541     471

  Fragments arranged by size: 

                1517     541     471
 
PstI C_TGCA'G

Cuts at:      0    1079    1448    1571    1832    2039    2139    2529 
   Size:       1079     369     123     261     207     100     390

  Fragments arranged by size: 

                1079     390     369     261     207     123     100
 
PvuII CAG'CTG

Cuts at:      0      96     222     394     792    2107    2529 
   Size:         96     126     172     398    1315     422

  Fragments arranged by size: 

                1315     422     398     172     126      96
 
SacI G_AGCT'C

Cuts at:      0      86    2225    2529 
   Size:         86    2139     304

  Fragments arranged by size: 

                2139     304      86
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1628    2287    2529 
   Size:       1628     659     242

  Fragments arranged by size: 

                1628     659     242
 
SfcI C'TryA_G

Cuts at:      0     405    1075    1444    1486    1567    1828    2035    2135 
   Size:        405     670     369      42      81     261     207     100

Cuts at:   2135    2337    2529 
   Size:        202     192

  Fragments arranged by size: 

                 670     405     369     261     207     202     192     100
                  81      42
 
SmaI CCC'GGG

Cuts at:      0     364    2529 
   Size:        364    2165
 
SmlI C'TyrA_G

Cuts at:      0     271     962    1995    2529 
   Size:        271     691    1033     534

  Fragments arranged by size: 

                1033     691     534     271
 
SphI G_CATG'C

Cuts at:      0    2301    2529 
   Size:       2301     228
 
SspI AAT'ATT

Cuts at:      0     591    2529 
   Size:        591    1938
 
StuI AGG'CCT

Cuts at:      0    2266    2529 
   Size:       2266     263
 
StyI C'CwwG_G

Cuts at:      0    2471    2529 
   Size:       2471      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     275    1548    2299    2529 
   Size:        275    1273     751     230

  Fragments arranged by size: 

                1273     751     275     230
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     841     873     915     947    2529 
   Size:        841      32      42      32    1582

  Fragments arranged by size: 

                1582     841      42      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     123     303    1320    2529 
   Size:        123     180    1017    1209

  Fragments arranged by size: 

                1209    1017     180     123
 
XmaI C'CCGG_G

Cuts at:      0     362    2529 
   Size:        362    2167
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AflII     AleI     AlfI
    AlwNI     ApaI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BssSI
   BstAPI    BstXI    BstYI   Bsu36I    BtgZI     BtsI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII
    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I     NspI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI     PstI    PvuII     SacI
     SapI     SfcI     SmaI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI   AflIII     AgeI     AhdI     AloI    ApaLI     AscI
     AseI    AsiSI    AvrII     BbeI     BbsI    BbvCI     BcgI    BfrBI
     BmtI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrFI    BsrGI   BssHII    BstBI   BstEII  BstZ17I
     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PpiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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