Restriction map for pF1KB7660
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AclI        AfaI        AflII       
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BlnI        BpuAI       BsaAI       BsaBI       BsgI        
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     ClaI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MseI        MslI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          712  GTAGAC               GT^[AC][GT]AC
AccIII        934  TCCGGA               T^CCGGA
AcsI          332  GAATTC               [GA]^AATT[CT]
AflIII        271  ACGTGT               A^C[GA][CT]GT
AgeI          598  ACCGGT               A^CCGGT
AlwNI         587  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          332  GAATTC               [GA]^AATT[CT]
AspHI         740  GTGCTC               G[AT]GC[AT]^C
BamHI          84  GGATCC               G^GATCC
BfaI          910  CTAG                 C^TAG
BglII         364  AGATCT               A^GATCT
BlpI         1123  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1123  GCTCAGC              GC^T[ACGT]AGC
BseAI         934  TCCGGA               T^CCGGA
BsiEI         261  CGGTCG               CG[GA][CT]^CG
BsiHKAI       740  GTGCTC               G[AT]GC[AT]^C
BspEI         934  TCCGGA               T^CCGGA
BspMI         759  GCAGGT               ACCTGC(4/8)
BsrFI         598  ACCGGT               [GA]^CCGG[CT]
BssHII       1095  GCGCGC               G^CGCGC
BstEII        904  GGTCACC              G^GT[ACGT]ACC
BstPI         904  GGTCACC              G^GT[ACGT]ACC
BstXI         974  CCATGGACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1123  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        598  ACCGGT               [GA]^CCGG[CT]
CpoI         1007  CGGTCCG              CG^G[AT]CCG
EcoO65I       904  GGTCACC              G^GT[ACGT]ACC
EcoRI         332  GAATTC               G^AATTC
HgaI           41  GCGTC                GACGC(5/10)
KspI          258  CCGCGG               CCGC^GG
MaeI          910  CTAG                 C^TAG
MaeII         271  ACGT                 A^CGT
MroI          934  TCCGGA               T^CCGGA
NcoI          974  CCATGG               C^CATGG
NspI          716  ACATGC               [GA]CATG^[CT]
PinAI         598  ACCGGT               A^CCGGT
RsrII        1007  CGGTCCG              CG^G[AT]CCG
SacII         258  CCGCGG               CCGC^GG
SfaNI         814  GATGC                GCATC(5/9)
SfcI          710  CTGTAG               C^T[GA][CT]AG
SmaI          620  CCCGGG               CCC^GGG
StuI          426  AGGCCT               AGG^CCT
TfiI          373  GATTC                G^A[AT]TC
XmaCI         620  CCCGGG               C^CCGGG
XmaI          620  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII         259  CGCG                 CG^CG
             1096  CGCG                
AcyI          352  GGCGCC               G[GA]^CG[CT]C
              406  GGCGCC              
AocI          344  CCTCAGG              CC^T[ACGT]AGG
              481  CCTCAGG             
AvaI          620  CCCGGG               C^[CT]CG[GA]G
             1137  CTCGGG              
BbeI          352  GGCGCC               GGCGC^C
              406  GGCGCC              
BglI          941  GCCTGGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1167  GCCCAATGGGC         
BsaHI         352  GGCGCC               G[GA]^CG[CT]C
              406  GGCGCC              
BsaI          293  GAGACC               GGTCTC(1/5)
              595  GAGACC              
BsaWI         598  ACCGGT               [AT]^CCGG[AT]
              934  TCCGGA              
BsoBI         620  CCCGGG               C^[CT]CG[GA]G
             1137  CTCGGG              
BsrI          499  ACTGG                ACTGG(1/-1)
              507  ACTGG               
BstUI         259  CGCG                 CG^CG
             1096  CGCG                
Bsu36I        344  CCTCAGG              CC^T[ACGT]AGG
              481  CCTCAGG             
EarI           18  GAAGAG               CTCTTC(1/4)
              748  GAAGAG              
Eco81I        344  CCTCAGG              CC^T[ACGT]AGG
              481  CCTCAGG             
EcoNI         429  CCTGTCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              641  CCTCTCTGAGG         
EcoT14I       536  CCAAGG               C^C[AT][AT]GG
              974  CCATGG              
Hin1I         352  GGCGCC               G[GA]^CG[CT]C
              406  GGCGCC              
KasI          352  GGCGCC               G^GCGCC
              406  GGCGCC              
Ksp632I        18  GAAGAG               CTCTTC(1/4)
              748  GAAGAG              
MaeIII         43  GTCAC                ^GT[ACGT]AC
              905  GTCAC               
MvnI          259  CGCG                 CG^CG
             1096  CGCG                
NarI          352  GGCGCC               GG^CGCC
              406  GGCGCC              
PleI          320  GACTC                GAGTC(4/5)
              775  GACTC               
PpuMI         682  AGGACCC              [GA]G^G[AT]CC[CT]
             1142  GGGTCCC             
SfoI          352  GGCGCC               GGC^GCC
              406  GGCGCC              
StyI          536  CCAAGG               C^C[AT][AT]GG
              974  CCATGG              
TaqI          336  TCGA                 T^CGA
              965  TCGA                
Tsp45I         43  GTCAC                ^GT[GC]AC
              905  GTCAC               
Tsp509I       333  AATT                 ^AATT
             1049  AATT                
TthHB8I       336  TCGA                 T^CGA
              965  TCGA                
XcmI          377  CCAGGTTGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              392  CCAGCCTCGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AluI        AlwI        ApaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrBI       
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MboI        MboII       MflI        MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PvuII       Sau3AI      Sau96I      ScrFI       
TseI        TspRI       Van91I      XhoII       
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