Restriction map for pF1KE2418
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflIII      
AhdI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BciVI       
BfaI        BglI        BglII       BlnI        BsaAI       
BseAI       BsiWI       BsmI        BspDI       BspEI       
BspLU11I    BsrDI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     ClaI        CpoI        DraI        
Eam1105I    Eco47III    EcoRI       EcoRV       EcoT22I     
FseI        FspI        HindIII     HpaI        KasI        
KpnI        MaeI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        ScaI        SexAI       SfiI        SfoI        
SfuI        SnaBI       SpeI        SphI        SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         679  GACGTC               GACGT^C
AflII        2140  CTTAAG               C^TTAAG
AocI          327  CCTCAGG              CC^T[ACGT]AGG
ApaI         1126  GGGCCC               GGGCC^C
AspI          298  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1571  TGGCCA               TGG^CCA
BbvCI        2479  GCTGAGG              CCTCAGC(-5/-2)
BclI         1337  TGATCA               T^GATCA
BfrI         2140  CTTAAG               C^TTAAG
BlpI         2422  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2422  GCTCAGC              GC^T[ACGT]AGC
BspHI        1700  TCATGA               T^CATGA
BsrBI        2163  CCGCTC               CCGCTC(-3/-3)
BssSI         193  CACGAG               CACGAG(-5/-1)
BstEII        651  GGTGACC              G^GT[ACGT]ACC
BstPI         651  GGTGACC              G^GT[ACGT]ACC
Bsu36I        327  CCTCAGG              CC^T[ACGT]AGG
CelII        2422  GCTCAGC              GC^T[ACGT]AGC
DraIII       1110  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        327  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       651  GGTGACC              G^GT[ACGT]ACC
FbaI         1337  TGATCA               T^GATCA
HaeII         835  AGCGCC               [GA]GCGC^[CT]
KspI         1668  CCGCGG               CCGC^GG
MluNI        1571  TGGCCA               TGG^CCA
MscI         1571  TGGCCA               TGG^CCA
PaeR7I       2405  CTCGAG               C^TCGAG
PflFI         298  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         1700  TCATGA               T^CATGA
SacI          982  GAGCTC               GAGCT^C
SacII        1668  CCGCGG               CCGC^GG
SgrAI         273  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         2289  CCCGGG               CCC^GGG
StuI         2156  AGGCCT               AGG^CCT
Tth111I       298  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2405  CTCGAG               C^TCGAG
XmaCI        2289  CCCGGG               C^CCGGG
XmaI         2289  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AgeI          274  ACCGGT               A^CCGGT
             2216  ACCGGT              
Alw44I        771  GTGCAC               G^TGCAC
             1132  GTGCAC              
ApaLI         771  GTGCAC               G^TGCAC
             1132  GTGCAC              
Asp700I      2130  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2154  GAAGGCCTTC          
BanI          277  GGTGCC               G^G[CT][GA]CC
             1091  GGCACC              
BsaBI         936  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1683  GATGGGCATC          
BsaWI         274  ACCGGT               [AT]^CCGG[AT]
             2216  ACCGGT              
BsmBI         263  GAGACG               CGTCTC(1/5)
              633  GAGACG              
Bsp1407I      926  TGTACA               T^GTACA
             1331  TGTACA              
BsrFI         274  ACCGGT               [GA]^CCGG[CT]
             2216  ACCGGT              
BsrGI         926  TGTACA               T^GTACA
             1331  TGTACA              
Cfr10I        274  ACCGGT               [GA]^CCGG[CT]
             2216  ACCGGT              
DraII        1851  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1864  AGGCCCC             
DrdI         1282  GACAGATTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2476  GACGCTGAGGTC        
EcoO109I     1851  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1864  AGGCCCC             
HincII        571  GTCAAC               GT[CT]^[GA]AC
             2059  GTCAAC              
HindII        571  GTCAAC               GT[CT]^[GA]AC
             2059  GTCAAC              
MamI          936  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1683  GATGGGCATC          
PflMI         381  CCAGGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1677  CCAGCAGATGG         
PinAI         274  ACCGGT               A^CCGGT
             2216  ACCGGT              
SbfI         1266  CCTGCAGG             CCTGCA^GG
             1859  CCTGCAGG            
Sse8387I     1266  CCTGCAGG             CCTGCA^GG
             1859  CCTGCAGG            
SspBI         926  TGTACA               T^GTACA
             1331  TGTACA              
Tsp509I      1404  AATT                 ^AATT
             2004  AATT                
Van91I        381  CCAGGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1677  CCAGCAGATGG         
XcmI          839  CCAGGGACTCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2108  CCATCACCATCCTGG     
XmnI         2130  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2154  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoNI       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      TspRI       TthHB8I     
XhoII       
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