Restriction map for
pF1KE2418
(Circular) (Six-base) MAPSORT of: pF1KE2418.seq Check: 7586 from: 1 to: 5630
pF1KE2418 5630 bp
With 182 enzymes: SgfI *
October 12, 2018 16:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 175 1285 2017 2280 175
Size: 1110 732 263 3525
Fragments arranged by size:
3525 1110 732 263
AatII G_ACGT'C
Cuts at: 791 791
Size: 5630
AccI GT'mk_AC
Cuts at: 1430 1565 1856 2668 1430
Size: 135 291 812 4392
Fragments arranged by size:
4392 812 291 135
Acc65I G'GTAC_C
Cuts at: 2646 2646
Size: 5630
AclI AA'CG_TT
Cuts at: 5470 5470
Size: 5630
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1099 1683 2319 2593 3455 3887 4634 4902
Size: 411 584 636 274 862 432 747 268
Cuts at: 4902 688
Size: 1416
Fragments arranged by size:
1416 862 747 636 584 432 411 274
268
AflII C'TTAA_G
Cuts at: 2248 2248
Size: 5630
AflIII A'CryG_T
Cuts at: 2982 4086 5063 2982
Size: 1104 977 3549
Fragments arranged by size:
3549 1104 977
AgeI A'CCGG_T
Cuts at: 382 2324 4018 382
Size: 1942 1694 1994
Fragments arranged by size:
1994 1942 1694
AleI CACnn'nnGTG
Cuts at: 1687 1888 2374 1687
Size: 201 486 4943
Fragments arranged by size:
4943 486 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 985 2302 985
Size: 1317 4313
AlwNI CAG_nnn'CTG
Cuts at: 495 1668 2468 2935 4502 495
Size: 1173 800 467 1567 1623
Fragments arranged by size:
1623 1567 1173 800 467
ApaI G_GGCC'C
Cuts at: 1238 1238
Size: 5630
ApaLI G'TGCA_C
Cuts at: 879 1240 4400 879
Size: 361 3160 2109
Fragments arranged by size:
3160 2109 361
ApoI r'AATT_y
Cuts at: 2634 4983 5239 2634
Size: 2349 256 3025
Fragments arranged by size:
3025 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5630
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
AvaI C'yCGr_G
Cuts at: 1189 2397 2513 2536 2650 1189
Size: 1208 116 23 114 4169
Fragments arranged by size:
4169 1208 116 114 23
BamHI G'GATC_C
Cuts at: 2655 2655
Size: 5630
BanI G'GyrC_C
Cuts at: 385 1199 2646 3060 3308 3343 385
Size: 814 1447 414 248 35 2672
Fragments arranged by size:
2672 1447 814 414 248 35
BanII G_rGCy'C
Cuts at: 1094 1190 1238 2644 3674 1094
Size: 96 48 1406 1030 3050
Fragments arranged by size:
3050 1406 1030 96 48
BbeI G_GCGC'C
Cuts at: 3064 3312 3064
Size: 248 5382
BbsI GAAGACnn'nnnn_
Cuts at: 436 580 1624 1857 1913 2076 2399 2586 436
Size: 144 1044 233 56 163 323 187 3480
Fragments arranged by size:
3480 1044 323 233 187 163 144 56
BbvCI CC'TCA_GC
Cuts at: 2588 2588
Size: 5630
BciVI GTATCCnnnnn_n'
Cuts at: 3528 4289 5630 3528
Size: 761 1341 3528
Fragments arranged by size:
3528 1341 761
BclI T'GATC_A
Cuts at: 1445 3150 1445
Size: 1705 3925
BglII A'GATC_T
Cuts at: 3145 5182 3145
Size: 2037 3593
BlpI GC'TnA_GC
Cuts at: 2531 2742 2531
Size: 211 5419
Bme1580I G_kGCm'C
Cuts at: 883 1238 1244 3255 3348 4404 883
Size: 355 6 2011 93 1056 2109
Fragments arranged by size:
2109 2011 1056 355 93 6
BmgBI CAC'GTC
Cuts at: 1943 1943
Size: 5630
BmrI ACTGGGnnnn_n'
Cuts at: 2842 2923 3258 5402 2842
Size: 81 335 2144 3070
Fragments arranged by size:
3070 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 336 1305 3997 336
Size: 969 2692 1969
Fragments arranged by size:
2692 1969 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 194 517 661 1105 1153 1318 1556 2035
Size: 11 323 144 444 48 165 238 479
Cuts at: 2035 2071 183
Size: 36 3742
Fragments arranged by size:
3742 479 444 323 238 165 144 48
36 11
Bpu10I CC'TnA_GC
Cuts at: 2274 2428 2588 2274
Size: 154 160 5316
Fragments arranged by size:
5316 160 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2806 3685 4177 4475 1693
Size: 1113 879 492 298 2848
Fragments arranged by size:
2848 1113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 182 1318 1826 2573 5334 182
Size: 1136 508 747 2761 478
Fragments arranged by size:
2761 1136 747 508 478
BsaAI yAC'GTr
Cuts at: 2983 2983
Size: 5630
BsaBI GATnn'nnATC
Cuts at: 6 1048 1795 3144 5181 6
Size: 1042 747 1349 2037 455
Fragments arranged by size:
2037 1349 1042 747 455
BsaHI Gr'CG_yC
Cuts at: 788 1114 2414 3061 3309 4011 5507 788
Size: 326 1300 647 248 702 1496 911
Fragments arranged by size:
1496 1300 911 702 647 326 248
BsaWI w'CCGG_w
Cuts at: 382 2324 2819 3340 4018 4292 4439 4850 382
Size: 1942 495 521 678 274 147 411 1162
Fragments arranged by size:
1942 1162 678 521 495 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 773 803 1177 1207 181
Size: 30 562 30 374 30 4604
Fragments arranged by size:
4604 562 374 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 1102 1236 1574 289
Size: 813 134 338 4345
Fragments arranged by size:
4345 813 338 134
BseYI C'CCAG_C
Cuts at: 221 891 1132 3055 3813 4390 4886 221
Size: 670 241 1923 758 577 496 965
Fragments arranged by size:
1923 965 758 670 577 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 958 1051 1215 709
Size: 249 93 164 5124
Fragments arranged by size:
5124 249 164 93
BsiEI CG_ry'CG
Cuts at: 104 935 1007 1609 3172 3218 4426 104
Size: 831 72 602 1563 46 1208 1308
Fragments arranged by size:
1563 1308 1208 831 602 72 46
BsiHKAI G_wGCw'C
Cuts at: 883 1094 1244 2514 2644 3422 3612 4404 883
Size: 211 150 1270 130 778 190 792 2109
Fragments arranged by size:
2109 1270 792 778 211 190 150 130
BsmBI CGTCTCn'nnnn_
Cuts at: 365 735 365
Size: 370 5260
Bsp1286I G_dGCh'C
Cuts at: 883 1094 1190 1238 1244 2514 2644 3255 3348
Size: 211 96 48 6 1270 130 611 93
Cuts at: 3348 3422 3612 3674 4404 883
Size: 74 190 62 730 2109
Fragments arranged by size:
2109 1270 730 611 211 190 130 96
93 74 62 48 6
BspEI T'CCGG_A
Cuts at: 4850 4850
Size: 5630
BspHI T'CATG_A
Cuts at: 1808 4806 1808
Size: 2998 2632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 175 611 1285 2017 2280 2680 2894 3196 3577
Size: 436 674 732 263 400 214 302 381
Cuts at: 3577 175
Size: 2228
Fragments arranged by size:
2228 732 674 436 400 381 302 263
214
BsrBI CCG'CTC
Cuts at: 50 2273 3922 3976 5460 5625 50
Size: 2223 1649 54 1484 165 55
Fragments arranged by size:
2223 1649 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3542 3542
Size: 5630
BsrFI r'CCGG_y
Cuts at: 382 2324 3628 3809 4018 382
Size: 1942 1304 181 209 1994
Fragments arranged by size:
1994 1942 1304 209 181
BsrGI T'GTAC_A
Cuts at: 1034 1439 1034
Size: 405 5225
BssSI C'ACGA_G
Cuts at: 301 3901 4259 301
Size: 3600 358 1672
Fragments arranged by size:
3600 1672 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3007 3007
Size: 5630
BstBI TT'CG_AA
Cuts at: 3991 3991
Size: 5630
BstEII G'GTnAC_C
Cuts at: 759 759
Size: 5630
BstXI CCAn_nnnn'nTGG
Cuts at: 891 1890 2013 2040 891
Size: 999 123 27 4481
Fragments arranged by size:
4481 999 123 27
BstYI r'GATC_y
Cuts at: 1 492 1178 1541 2655 3122 3145 3480 3726
Size: 491 686 363 1114 467 23 335 246
Cuts at: 3726 4738 4824 4836 5182 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1114 1012 686 491 467 449 363 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 436 436
Size: 5630
BtgI C'CryG_G
Cuts at: 230 804 1457 1478 1685 1727 1776 230
Size: 574 653 21 207 42 49 4084
Fragments arranged by size:
4084 653 574 207 49 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 714 2130 3580 3761 3924 714
Size: 1416 1450 181 163 2420
Fragments arranged by size:
2420 1450 1416 181 163
BtsI GCAGTG_nn'
Cuts at: 721 2873 721
Size: 2152 3478
DraI TTT'AAA
Cuts at: 2629 2629
Size: 5630
DraIII CAC_nnn'GTG
Cuts at: 1223 1223
Size: 5630
DrdI GACnn_nn'nnGTC
Cuts at: 1396 2590 3336 4194 1396
Size: 1194 746 858 2832
Fragments arranged by size:
2832 1194 858 746
EaeI y'GGCC_r
Cuts at: 233 872 932 992 1004 1606 1679 3215 3389
Size: 639 60 60 12 602 73 1536 174
Cuts at: 3389 3780 3807 5152 233
Size: 391 27 1345 711
Fragments arranged by size:
1536 1345 711 639 602 391 174 73
60 60 27 12
EagI C'GGCC_G
Cuts at: 932 1004 1606 3215 932
Size: 72 602 1609 3347
Fragments arranged by size:
3347 1609 602 72
EarI CTCTTCn'nnn_
Cuts at: 517 2233 3653 3863 517
Size: 1716 1420 210 2284
Fragments arranged by size:
2284 1716 1420 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3838 4148 4294 5440 3838
Size: 310 146 1146 4028
Fragments arranged by size:
4028 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1092 2642 1092
Size: 1550 4080
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 194 517 661 688 1099 1105 1153 1318
Size: 11 323 144 27 411 6 48 165
Cuts at: 1318 1556 1683 2035 2071 2319 2593 3455 3887
Size: 238 127 352 36 248 274 862 432
Cuts at: 3887 4634 4902 183
Size: 747 268 911
Fragments arranged by size:
911 862 747 432 411 352 323 274
268 248 238 165 144 127 48 36
27 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 566 985 1470 566
Size: 419 485 4726
Fragments arranged by size:
4726 485 419
EcoO109I rG'GnC_Cy
Cuts at: 1960 1973 2769 1960
Size: 13 796 4821
Fragments arranged by size:
4821 796 13
EcoRI G'AATT_C
Cuts at: 2634 2634
Size: 5630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1425 2100 3649 1425
Size: 675 1549 3406
Fragments arranged by size:
3406 1549 675
FspI TGC'GCA
Cuts at: 3411 3411
Size: 5630
HaeII r_GCGC'y
Cuts at: 947 3064 3312 4334 5313 947
Size: 2117 248 1022 979 1264
Fragments arranged by size:
2117 1264 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 773 805 1317 1349 1531 1563 1927
Size: 32 560 32 512 32 182 32 364
Cuts at: 1927 1959 2509 2541 3472 3502 3504 3534 181
Size: 32 550 32 931 30 2 30 2277
Fragments arranged by size:
2277 931 560 550 512 364 182 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 681 2169 2669 681
Size: 1488 500 3642
Fragments arranged by size:
3642 1488 500
KasI G'GCGC_C
Cuts at: 3060 3308 3060
Size: 248 5382
KpnI G_GTAC'C
Cuts at: 2650 2650
Size: 5630
MluI A'CGCG_T
Cuts at: 5063 5063
Size: 5630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4301 4485 4301
Size: 184 5446
MscI TGG'CCA
Cuts at: 1681 3391 1681
Size: 1710 3920
MslI CAynn'nnrTG
Cuts at: 1687 1888 2038 2374 3619 3746 1687
Size: 201 150 336 1245 127 3571
Fragments arranged by size:
3571 1245 336 201 150 127
MspA1I CmG'CkG
Cuts at: 142 891 1119 1132 1173 1362 1778 1888 2741
Size: 749 228 13 41 189 416 110 853
Cuts at: 2741 3055 3415 4428 4673 142
Size: 314 360 1013 245 1099
Fragments arranged by size:
1099 1013 853 749 416 360 314 245
228 189 110 41 13
NaeI GCC'GGC
Cuts at: 3811 3811
Size: 5630
NarI GG'CG_CC
Cuts at: 3061 3309 3061
Size: 248 5382
NcoI C'CATG_G
Cuts at: 804 1457 1727 804
Size: 653 270 4707
Fragments arranged by size:
4707 653 270
NgoMIV G'CCGG_C
Cuts at: 3809 3809
Size: 5630
NspI r_CATG'y
Cuts at: 1035 1347 1764 2142 2683 3183 4090 1035
Size: 312 417 378 541 500 907 2575
Fragments arranged by size:
2575 907 541 500 417 378 312
PciI A'CATG_T
Cuts at: 4086 4086
Size: 5630
PflMI CCAn_nnn'nTGG
Cuts at: 495 1791 495
Size: 1296 4334
PfoI T'CCnGG_A
Cuts at: 638 1181 1207 2225 638
Size: 543 26 1018 4043
Fragments arranged by size:
4043 1018 543 26
PmeI GTTT'AAAC
Cuts at: 2629 2629
Size: 5630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4769 4801 4769
Size: 32 5598
PspOMI G'GGCC_C
Cuts at: 1234 1234
Size: 5630
PstI C_TGCA'G
Cuts at: 521 917 1379 1601 1664 1972 2210 2291 2677
Size: 396 462 222 63 308 238 81 386
Cuts at: 2677 3362 521
Size: 685 2789
Fragments arranged by size:
2789 685 462 396 386 308 238 222
81 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5630
PvuII CAG'CTG
Cuts at: 142 891 1132 3055 3415 142
Size: 749 241 1923 360 2357
Fragments arranged by size:
2357 1923 749 360 241
RsrII CG'GwC_CG
Cuts at: 3825 3825
Size: 5630
SacI G_AGCT'C
Cuts at: 1094 2644 1094
Size: 1550 4080
SacII CC_GC'GG
Cuts at: 1779 1779
Size: 5630
SalI G'TCGA_C
Cuts at: 2667 2667
Size: 5630
SapI GCTCTTCn'nnn_
Cuts at: 517 2233 3653 3863 517
Size: 1716 1420 210 2284
Fragments arranged by size:
2284 1716 1420 210
SbfI CC_TGCA'GG
Cuts at: 1379 1972 2677 1379
Size: 593 705 4332
Fragments arranged by size:
4332 705 593
ScaI AGT'ACT
Cuts at: 5147 5147
Size: 5630
SfcI C'TryA_G
Cuts at: 33 517 913 1311 1375 1470 1597 1638 1660
Size: 484 396 398 64 95 127 41 22
Cuts at: 1660 1968 2206 2287 2673 3358 4351 4542 33
Size: 308 238 81 386 685 993 191 1121
Fragments arranged by size:
1121 993 685 484 398 396 386 308
238 191 127 95 81 64 41 22
SfoI GGC'GCC
Cuts at: 3062 3310 3062
Size: 248 5382
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
SgrAI Cr'CCGG_yG
Cuts at: 382 382
Size: 5630
SmaI CCC'GGG
Cuts at: 2399 2652 2399
Size: 253 5377
SmlI C'TyrA_G
Cuts at: 1708 2248 2513 2785 3700 4192 4454 1708
Size: 540 265 272 915 492 262 2884
Fragments arranged by size:
2884 915 540 492 272 265 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5630
SphI G_CATG'C
Cuts at: 2683 3183 2683
Size: 500 5130
StuI AGG'CCT
Cuts at: 2266 2266
Size: 5630
StyI C'CwwG_G
Cuts at: 804 1457 1727 1745 2764 3118 804
Size: 653 270 18 1019 354 3316
Fragments arranged by size:
3316 1019 653 354 270 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2146 3213 3346 3805 4014 2146
Size: 1067 133 459 209 3762
Fragments arranged by size:
3762 1067 459 209 133
TatI w'GTAC_w
Cuts at: 1034 1439 1694 2000 5145 1034
Size: 405 255 306 3145 1519
Fragments arranged by size:
3145 1519 405 306 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1177 1209 5147 5179 1177
Size: 32 3938 32 1628
Fragments arranged by size:
3938 1628 32 32
Tth111I GACn'n_nGTC
Cuts at: 409 3427 409
Size: 3018 2612
XbaI T'CTAG_A
Cuts at: 2661 2661
Size: 5630
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 954 2223 3125 954
Size: 1269 902 3459
Fragments arranged by size:
3459 1269 902
XhoI C'TCGA_G
Cuts at: 2513 2513
Size: 5630
XmaI C'CCGG_G
Cuts at: 2397 2650 2397
Size: 253 5377
XmnI GAAnn'nnTTC
Cuts at: 2242 2266 2242
Size: 24 5606
ZraI GAC'GTC
Cuts at: 789 789
Size: 5630
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI AvrII BaeI BcgI BfrBI
BglI BmtI BsiWI BsmI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SnaBI SrfI
SspI SwaI