Restriction map for pF1KE2418
 (Linear) (Six-base) MAPSORT of: KIEE2418.seq  Check: 7845  from: 1  to: 2517



KIEE2418.orf

 With 182 enzymes: SgfI * 

                        October 12, 2018 16:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      67    1177    1909    2172    2517 
   Size:         67    1110     732     263     345

  Fragments arranged by size: 

                1110     732     345     263      67
 
AatII G_ACGT'C

Cuts at:      0     683    2517 
   Size:        683    1834
 
AccI GT'mk_AC

Cuts at:      0    1322    1457    1748    2517 
   Size:       1322     135     291     769

  Fragments arranged by size: 

                1322     769     291     135
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     991    1575    2211    2485    2517 
   Size:        580     411     584     636     274      32

  Fragments arranged by size: 

                 636     584     580     411     274      32
 
AflII C'TTAA_G

Cuts at:      0    2140    2517 
   Size:       2140     377
 
AgeI A'CCGG_T

Cuts at:      0     274    2216    2517 
   Size:        274    1942     301

  Fragments arranged by size: 

                1942     301     274
 
AleI CACnn'nnGTG

Cuts at:      0    1579    1780    2266    2517 
   Size:       1579     201     486     251

  Fragments arranged by size: 

                1579     486     251     201
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     877    2194    2517 
   Size:        877    1317     323

  Fragments arranged by size: 

                1317     877     323
 
AlwNI CAG_nnn'CTG

Cuts at:      0     387    1560    2360    2517 
   Size:        387    1173     800     157

  Fragments arranged by size: 

                1173     800     387     157
 
ApaI G_GGCC'C

Cuts at:      0    1130    2517 
   Size:       1130    1387
 
ApaLI G'TGCA_C

Cuts at:      0     771    1132    2517 
   Size:        771     361    1385

  Fragments arranged by size: 

                1385     771     361
 
AvaI C'yCGr_G

Cuts at:      0    1081    2289    2405    2428    2517 
   Size:       1081    1208     116      23      89

  Fragments arranged by size: 

                1208    1081     116      89      23
 
BanI G'GyrC_C

Cuts at:      0     277    1091    2517 
   Size:        277     814    1426

  Fragments arranged by size: 

                1426     814     277
 
BanII G_rGCy'C

Cuts at:      0     986    1082    1130    2517 
   Size:        986      96      48    1387

  Fragments arranged by size: 

                1387     986      96      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     328     472    1516    1749    1805    1968    2291    2478 
   Size:        328     144    1044     233      56     163     323     187

Cuts at:   2478    2517 
   Size:         39

  Fragments arranged by size: 

                1044     328     323     233     187     163     144      56
                  39
 
BbvCI CC'TCA_GC

Cuts at:      0    2480    2517 
   Size:       2480      37
 
BclI T'GATC_A

Cuts at:      0    1337    2517 
   Size:       1337    1180
 
BlpI GC'TnA_GC

Cuts at:      0    2423    2517 
   Size:       2423      94
 
Bme1580I G_kGCm'C

Cuts at:      0     775    1130    1136    2517 
   Size:        775     355       6    1381

  Fragments arranged by size: 

                1381     775     355       6
 
BmgBI CAC'GTC

Cuts at:      0    1835    2517 
   Size:       1835     682
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     228    1197    2517 
   Size:        228     969    1320

  Fragments arranged by size: 

                1320     969     228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75      86     409     553     997    1045    1210    1448 
   Size:         75      11     323     144     444      48     165     238

Cuts at:   1448    1927    1963    2517 
   Size:        479      36     554

  Fragments arranged by size: 

                 554     479     444     323     238     165     144      75
                  48      36      11
 
Bpu10I CC'TnA_GC

Cuts at:      0    2166    2320    2480    2517 
   Size:       2166     154     160      37

  Fragments arranged by size: 

                2166     160     154      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1585    2517 
   Size:       1585     932
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      74    1210    1718    2465    2517 
   Size:         74    1136     508     747      52

  Fragments arranged by size: 

                1136     747     508      74      52
 
BsaBI GATnn'nnATC

Cuts at:      0     940    1687    2517 
   Size:        940     747     830

  Fragments arranged by size: 

                 940     830     747
 
BsaHI Gr'CG_yC

Cuts at:      0     680    1006    2306    2517 
   Size:        680     326    1300     211

  Fragments arranged by size: 

                1300     680     326     211
 
BsaWI w'CCGG_w

Cuts at:      0     274    2216    2517 
   Size:        274    1942     301

  Fragments arranged by size: 

                1942     301     274
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      73     103     665     695    1069    1099    2517 
   Size:         73      30     562      30     374      30    1418

  Fragments arranged by size: 

                1418     562     374      73      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     181     994    1128    1466    2517 
   Size:        181     813     134     338    1051

  Fragments arranged by size: 

                1051     813     338     181     134
 
BseYI C'CCAG_C

Cuts at:      0     113     783    1024    2517 
   Size:        113     670     241    1493

  Fragments arranged by size: 

                1493     670     241     113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     850     943    1107    2517 
   Size:        601     249      93     164    1410

  Fragments arranged by size: 

                1410     601     249     164      93
 
BsiEI CG_ry'CG

Cuts at:      0     827     899    1501    2517 
   Size:        827      72     602    1016

  Fragments arranged by size: 

                1016     827     602      72
 
BsiHKAI G_wGCw'C

Cuts at:      0     775     986    1136    2406    2517 
   Size:        775     211     150    1270     111

  Fragments arranged by size: 

                1270     775     211     150     111
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     257     627    2517 
   Size:        257     370    1890

  Fragments arranged by size: 

                1890     370     257
 
Bsp1286I G_dGCh'C

Cuts at:      0     775     986    1082    1130    1136    2406    2517 
   Size:        775     211      96      48       6    1270     111

  Fragments arranged by size: 

                1270     775     211     111      96      48       6
 
BspHI T'CATG_A

Cuts at:      0    1700    2517 
   Size:       1700     817
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      67     503    1177    1909    2172    2517 
   Size:         67     436     674     732     263     345

  Fragments arranged by size: 

                 732     674     436     345     263      67
 
BsrBI CCG'CTC

Cuts at:      0    2165    2517 
   Size:       2165     352
 
BsrFI r'CCGG_y

Cuts at:      0     274    2216    2517 
   Size:        274    1942     301

  Fragments arranged by size: 

                1942     301     274
 
BsrGI T'GTAC_A

Cuts at:      0     926    1331    2517 
   Size:        926     405    1186

  Fragments arranged by size: 

                1186     926     405
 
BssSI C'ACGA_G

Cuts at:      0     193    2517 
   Size:        193    2324
 
BstEII G'GTnAC_C

Cuts at:      0     651    2517 
   Size:        651    1866
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     783    1782    1905    1932    2517 
   Size:        783     999     123      27     585

  Fragments arranged by size: 

                 999     783     585     123      27
 
BstYI r'GATC_y

Cuts at:      0     384    1070    1433    2517 
   Size:        384     686     363    1084

  Fragments arranged by size: 

                1084     686     384     363
 
Bsu36I CC'TnA_GG

Cuts at:      0     328    2517 
   Size:        328    2189
 
BtgI C'CryG_G

Cuts at:      0     122     696    1349    1370    1577    1619    1668    2517 
   Size:        122     574     653      21     207      42      49     849

  Fragments arranged by size: 

                 849     653     574     207     122      49      42      21
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     606    2022    2517 
   Size:        606    1416     495

  Fragments arranged by size: 

                1416     606     495
 
BtsI GCAGTG_nn'

Cuts at:      0     613    2517 
   Size:        613    1904
 
DraIII CAC_nnn'GTG

Cuts at:      0    1115    2517 
   Size:       1115    1402
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1288    2482    2517 
   Size:       1288    1194      35

  Fragments arranged by size: 

                1288    1194      35
 
EaeI y'GGCC_r

Cuts at:      0     125     764     824     884     896    1498    1571    2517 
   Size:        125     639      60      60      12     602      73     946

  Fragments arranged by size: 

                 946     639     602     125      73      60      60      12
            
 
EagI C'GGCC_G

Cuts at:      0     824     896    1498    2517 
   Size:        824      72     602    1019

  Fragments arranged by size: 

                1019     824     602      72
 
EarI CTCTTCn'nnn_

Cuts at:      0     409    2125    2517 
   Size:        409    1716     392

  Fragments arranged by size: 

                1716     409     392
 
EcoICRI GAG'CTC

Cuts at:      0     984    2517 
   Size:        984    1533
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75      86     409     553     580     991     997    1045 
   Size:         75      11     323     144      27     411       6      48

Cuts at:   1045    1210    1448    1575    1927    1963    2211    2485    2517 
   Size:        165     238     127     352      36     248     274      32

  Fragments arranged by size: 

                 411     352     323     274     248     238     165     144
                 127      75      48      36      32      27      11       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     458     877    1362    2517 
   Size:        458     419     485    1155

  Fragments arranged by size: 

                1155     485     458     419
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1852    1865    2517 
   Size:       1852      13     652

  Fragments arranged by size: 

                1852     652      13
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1317    1992    2517 
   Size:       1317     675     525

  Fragments arranged by size: 

                1317     675     525
 
HaeII r_GCGC'y

Cuts at:      0     839    2517 
   Size:        839    1678
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      73     105     665     697    1209    1241    1423    1455 
   Size:         73      32     560      32     512      32     182      32

Cuts at:   1455    1819    1851    2401    2433    2517 
   Size:        364      32     550      32      84

  Fragments arranged by size: 

                 560     550     512     364     182      84      73      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     573    2061    2517 
   Size:        573    1488     456

  Fragments arranged by size: 

                1488     573     456
 
MscI TGG'CCA

Cuts at:      0    1573    2517 
   Size:       1573     944
 
MslI CAynn'nnrTG

Cuts at:      0    1579    1780    1930    2266    2517 
   Size:       1579     201     150     336     251

  Fragments arranged by size: 

                1579     336     251     201     150
 
MspA1I CmG'CkG

Cuts at:      0      34     783    1011    1024    1065    1254    1670    1780 
   Size:         34     749     228      13      41     189     416     110

Cuts at:   1780    2517 
   Size:        737

  Fragments arranged by size: 

                 749     737     416     228     189     110      41      34
                  13
 
NcoI C'CATG_G

Cuts at:      0     696    1349    1619    2517 
   Size:        696     653     270     898

  Fragments arranged by size: 

                 898     696     653     270
 
NspI r_CATG'y

Cuts at:      0     927    1239    1656    2034    2517 
   Size:        927     312     417     378     483

  Fragments arranged by size: 

                 927     483     417     378     312
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     387    1683    2517 
   Size:        387    1296     834

  Fragments arranged by size: 

                1296     834     387
 
PfoI T'CCnGG_A

Cuts at:      0     530    1073    1099    2117    2517 
   Size:        530     543      26    1018     400

  Fragments arranged by size: 

                1018     543     530     400      26
 
PspOMI G'GGCC_C

Cuts at:      0    1126    2517 
   Size:       1126    1391
 
PstI C_TGCA'G

Cuts at:      0     413     809    1271    1493    1556    1864    2102    2183 
   Size:        413     396     462     222      63     308     238      81

Cuts at:   2183    2517 
   Size:        334

  Fragments arranged by size: 

                 462     413     396     334     308     238     222      81
                  63
 
PvuII CAG'CTG

Cuts at:      0      34     783    1024    2517 
   Size:         34     749     241    1493

  Fragments arranged by size: 

                1493     749     241      34
 
SacI G_AGCT'C

Cuts at:      0     986    2517 
   Size:        986    1531
 
SacII CC_GC'GG

Cuts at:      0    1671    2517 
   Size:       1671     846
 
SapI GCTCTTCn'nnn_

Cuts at:      0     409    2125    2517 
   Size:        409    1716     392

  Fragments arranged by size: 

                1716     409     392
 
SbfI CC_TGCA'GG

Cuts at:      0    1271    1864    2517 
   Size:       1271     593     653

  Fragments arranged by size: 

                1271     653     593
 
SfcI C'TryA_G

Cuts at:      0     409     805    1203    1267    1362    1489    1530    1552 
   Size:        409     396     398      64      95     127      41      22

Cuts at:   1552    1860    2098    2179    2517 
   Size:        308     238      81     338

  Fragments arranged by size: 

                 409     398     396     338     308     238     127      95
                  81      64      41      22
 
SgrAI Cr'CCGG_yG

Cuts at:      0     274    2517 
   Size:        274    2243
 
SmaI CCC'GGG

Cuts at:      0    2291    2517 
   Size:       2291     226
 
SmlI C'TyrA_G

Cuts at:      0    1600    2140    2405    2517 
   Size:       1600     540     265     112

  Fragments arranged by size: 

                1600     540     265     112
 
StuI AGG'CCT

Cuts at:      0    2158    2517 
   Size:       2158     359
 
StyI C'CwwG_G

Cuts at:      0     696    1349    1619    1637    2517 
   Size:        696     653     270      18     880

  Fragments arranged by size: 

                 880     696     653     270      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2038    2517 
   Size:       2038     479
 
TatI w'GTAC_w

Cuts at:      0     926    1331    1586    1892    2517 
   Size:        926     405     255     306     625

  Fragments arranged by size: 

                 926     625     405     306     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1069    1101    2517 
   Size:       1069      32    1416

  Fragments arranged by size: 

                1416    1069      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     301    2517 
   Size:        301    2216
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     846    2115    2517 
   Size:        846    1269     402

  Fragments arranged by size: 

                1269     846     402
 
XhoI C'TCGA_G

Cuts at:      0    2405    2517 
   Size:       2405     112
 
XmaI C'CCGG_G

Cuts at:      0    2289    2517 
   Size:       2289     228
 
XmnI GAAnn'nnTTC

Cuts at:      0    2134    2158    2517 
   Size:       2134      24     359

  Fragments arranged by size: 

                2134     359      24
 
ZraI GAC'GTC

Cuts at:      0     681    2517 
   Size:        681    1836
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AflII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI
     BclI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
    BsrGI    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     MscI     MslI
   MspA1I     NcoI     NspI    PflMI     PfoI   PspOMI     PstI    PvuII
     SacI    SacII     SapI     SbfI     SfcI    SgrAI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI   AflIII     AhdI     AloI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI
    BfrBI     BglI    BglII     BmrI     BmtI    BsaAI    BsiWI     BsmI
    BspEI    BsrDI   BssHII   BstAPI    BstBI  BstZ17I     ClaI    CspCI
     DraI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SalI    SanDI     ScaI    SexAI     SfiI
     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XbaI
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