Restriction map for pF1KB7885
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspI        AvaI        AviII       AvrII       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       BspDI       BspEI       BspMI       BsrFI       
BssHII      BssSI       Bst1107I    BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       EcoT22I     FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AflIII       1148  ACATGT               A^C[GA][CT]GT
AocI         1630  CCTCAGG              CC^T[ACGT]AGG
BalI          398  TGGCCA               TGG^CCA
BamHI         240  GGATCC               G^GATCC
BanI         1304  GGTGCC               G^G[CT][GA]CC
BclI          222  TGATCA               T^GATCA
BcnI           23  CCGGG                CC^[GC]GG
BglII        1108  AGATCT               A^GATCT
BsgI         1187  GTGCAG               GTGCAG(16/14)
Bsp1407I     1314  TGTACA               T^GTACA
BspHI         627  TCATGA               T^CATGA
BspLU11I     1148  ACATGT               A^CATGT
BsrBI         904  GAGCGG               CCGCTC(-3/-3)
BsrDI        1065  CATTGC               GCAATG(2/0)
BsrGI        1314  TGTACA               T^GTACA
BstAPI       1251  GCACCAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         830  TTCGAA               TT^CGAA
BstEII        975  GGTCACC              G^GT[ACGT]ACC
BstF5I         78  GGATG                GGATG(2/0)
BstPI         975  GGTCACC              G^GT[ACGT]ACC
BstXI        1669  CCAAAAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1630  CCTCAGG              CC^T[ACGT]AGG
Cac8I         480  GCTTGC               GC[ACGT]^[ACGT]GC
CfoI          564  GCGC                 GCG^C
EaeI          398  TGGCCA               [CT]^GGCC[GA]
Eco81I       1630  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1654  CCTATTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       975  GGTCACC              G^GT[ACGT]ACC
EcoRI         705  GAATTC               G^AATTC
FbaI          222  TGATCA               T^GATCA
FokI           78  GGATG                GGATG(9/13)
HapII          23  CCGG                 C^CGG
HhaI          564  GCGC                 GCG^C
HinP1I        564  GCGC                 G^CGC
HincII       1174  GTTGAC               GT[CT]^[GA]AC
HindII       1174  GTTGAC               GT[CT]^[GA]AC
HpaII          23  CCGG                 C^CGG
MaeII         104  ACGT                 A^CGT
MfeI          113  CAATTG               C^AATTG
MluNI         398  TGGCCA               TGG^CCA
MscI          398  TGGCCA               TGG^CCA
MspA1I         31  CCGCTG               C[AC]G^C[GT]G
MspI           23  CCGG                 C^CGG
MunI          113  CAATTG               C^AATTG
NciI           23  CCGGG                CC^[GC]GG
NspI         1148  ACATGT               [GA]CATG^[CT]
NspV          830  TTCGAA               TT^CGAA
PstI          371  CTGCAG               CTGCA^G
RcaI          627  TCATGA               T^CATGA
SapI         1117  GAAGAGC              GCTCTTC(1/4)
SfcI          371  CTGCAG               C^T[GA][CT]AG
SfuI          830  TTCGAA               TT^CGAA
SpeI          823  ACTAGT               A^CTAGT
SspBI        1314  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AhdI         1501  GACTATCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1543  GACTGCTTGTC         
Alw44I         37  GTGCAC               G^TGCAC
               45  GTGCAC              
ApaLI          37  GTGCAC               G^TGCAC
               45  GTGCAC              
Asp700I        63  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1203  GAAGGCTTTC          
AspEI        1501  GACTATCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1543  GACTGCTTGTC         
AspHI          37  GTGCAC               G[AT]GC[AT]^C
               45  GTGCAC              
BmyI           37  GTGCAC               G[AGT]GC[ACT]^C
               45  GTGCAC              
BsiHKAI        37  GTGCAC               G[AT]GC[AT]^C
               45  GTGCAC              
BsiYI        1384  CCCCACAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1654  CCTATTAAAGG         
BslI         1384  CCCCACAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1654  CCTATTAAAGG         
BsmAI        1051  GAGAC                GTCTC(1/5)
             1441  GTCTC               
Bsp1286I       37  GTGCAC               G[AGT]GC[ACT]^C
               45  GTGCAC              
Eam1105I     1501  GACTATCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1543  GACTGCTTGTC         
EarI          127  GAAGAG               CTCTTC(1/4)
             1117  GAAGAG              
EcoT14I       401  CCAAGG               C^C[AT][AT]GG
              731  CCAAGG              
FauI          309  GCGGG                CCCGC(4/6)
              906  GCGGG               
HphI          504  TCACC                GGTGA(8/7)
              977  TCACC               
Ksp632I       127  GAAGAG               CTCTTC(1/4)
             1117  GAAGAG              
PleI          655  GACTC                GAGTC(4/5)
              856  GAGTC               
PpuMI         734  AGGACCT              [GA]G^G[AT]CC[CT]
              835  AGGACCC             
PshAI         837  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1582  GACATCAGTC          
SmlI          901  CTTGAG               C^T[CT][GA]AG
             1433  CTCAAG              
StyI          401  CCAAGG               C^C[AT][AT]GG
              731  CCAAGG              
XmnI           63  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1203  GAAGGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BbsI        BbvI        
BfaI        BpmI        BpuAI       BsaJI       BseRI       
BsmFI       BsrI        BssKI       BstNI       BstYI       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      HaeIII      HinfI       ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com