Restriction map for pF1KB7885
 (Linear) (Six-base) MAPSORT of: KIBB7885.seq  Check: 9256  from: 1  to: 1743



KIBB7885.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1136    1202    1743 
   Size:        146     990      66     541

  Fragments arranged by size: 

                 990     541     146      66
 
AflIII A'CryG_T

Cuts at:      0    1148    1743 
   Size:       1148     595
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1506    1548    1743 
   Size:       1506      42     195

  Fragments arranged by size: 

                1506     195      42
 
AleI CACnn'nnGTG

Cuts at:      0     509     616    1184    1743 
   Size:        509     107     568     559

  Fragments arranged by size: 

                 568     559     509     107
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     406     438    1743 
   Size:        406      32    1305

  Fragments arranged by size: 

                1305     406      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     675    1092    1373    1743 
   Size:        675     417     281     370

  Fragments arranged by size: 

                 675     417     370     281
 
ApaLI G'TGCA_C

Cuts at:      0      37      45    1743 
   Size:         37       8    1698

  Fragments arranged by size: 

                1698      37       8
 
ApoI r'AATT_y

Cuts at:      0     272     705    1017    1276    1489    1743 
   Size:        272     433     312     259     213     254

  Fragments arranged by size: 

                 433     312     272     259     254     213
 
BamHI G'GATC_C

Cuts at:      0     240    1743 
   Size:        240    1503
 
BanI G'GyrC_C

Cuts at:      0    1304    1743 
   Size:       1304     439
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      70     442    1459    1510    1743 
   Size:         70     372    1017      51     233

  Fragments arranged by size: 

                1017     372     233      70      51
 
BclI T'GATC_A

Cuts at:      0     222    1743 
   Size:        222    1521
 
BglII A'GATC_T

Cuts at:      0    1108    1743 
   Size:       1108     635
 
Bme1580I G_kGCm'C

Cuts at:      0      41      49    1743 
   Size:         41       8    1694

  Fragments arranged by size: 

                1694      41       8
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1161    1743 
   Size:       1161     582
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1257    1743 
   Size:       1257     486
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     796    1435    1743 
   Size:        142     654     639     308

  Fragments arranged by size: 

                 654     639     308     142
 
Bpu10I CC'TnA_GC

Cuts at:      0     588    1743 
   Size:        588    1155
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922    1418    1743 
   Size:        922     496     325

  Fragments arranged by size: 

                 922     496     325
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     406     436    1121    1151    1743 
   Size:        406      30     685      30     592

  Fragments arranged by size: 

                 685     592     406      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     145     943     946    1743 
   Size:        103      42     798       3     797

  Fragments arranged by size: 

                 798     797     103      42       3
 
BseYI C'CCAG_C

Cuts at:      0      10      50    1743 
   Size:         10      40    1693

  Fragments arranged by size: 

                1693      40      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1208    1743 
   Size:       1208     535
 
BsiHKAI G_wGCw'C

Cuts at:      0      41      49    1743 
   Size:         41       8    1694

  Fragments arranged by size: 

                1694      41       8
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      49    1743 
   Size:         41       8    1694

  Fragments arranged by size: 

                1694      41       8
 
BspHI T'CATG_A

Cuts at:      0     627    1743 
   Size:        627    1116
 
BsrBI CCG'CTC

Cuts at:      0     906    1743 
   Size:        906     837
 
BsrDI GCAATG_nn'

Cuts at:      0    1064    1743 
   Size:       1064     679
 
BsrGI T'GTAC_A

Cuts at:      0    1314    1743 
   Size:       1314     429
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1257    1743 
   Size:       1257     486
 
BstBI TT'CG_AA

Cuts at:      0     831    1743 
   Size:        831     912
 
BstEII G'GTnAC_C

Cuts at:      0     975    1743 
   Size:        975     768
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1676    1743 
   Size:       1676      67
 
BstYI r'GATC_y

Cuts at:      0     240    1001    1108    1369    1694    1743 
   Size:        240     761     107     261     325      49

  Fragments arranged by size: 

                 761     325     261     240     107      49
 
Bsu36I CC'TnA_GG

Cuts at:      0    1631    1743 
   Size:       1631     112
 
BtsI GCAGTG_nn'

Cuts at:      0     259    1743 
   Size:        259    1484
 
EaeI y'GGCC_r

Cuts at:      0     398    1743 
   Size:        398    1345
 
EarI CTCTTCn'nnn_

Cuts at:      0     122    1112    1743 
   Size:        122     990     631

  Fragments arranged by size: 

                 990     631     122
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     146     796    1136    1202    1435    1743 
   Size:        142       4     650     340      66     233     308

  Fragments arranged by size: 

                 650     340     308     233     142      66       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1658    1743 
   Size:       1658      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0     592     735     836    1743 
   Size:        592     143     101     907

  Fragments arranged by size: 

                 907     592     143     101
 
EcoRI G'AATT_C

Cuts at:      0     705    1743 
   Size:        705    1038
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     225     257     841     873    1354    1386    1492    1524 
   Size:        225      32     584      32     481      32     106      32

Cuts at:   1524    1534    1566    1743 
   Size:         10      32     177

  Fragments arranged by size: 

                 584     481     225     177     106      32      32      32
                  32      32      10
 
HincII GTy'rAC

Cuts at:      0    1176    1743 
   Size:       1176     567
 
MfeI C'AATT_G

Cuts at:      0     113    1743 
   Size:        113    1630
 
MscI TGG'CCA

Cuts at:      0     400    1743 
   Size:        400    1343
 
MslI CAynn'nnrTG

Cuts at:      0     509     616    1184    1743 
   Size:        509     107     568     559

  Fragments arranged by size: 

                 568     559     509     107
 
MspA1I CmG'CkG

Cuts at:      0      33    1743 
   Size:         33    1710
 
NspI r_CATG'y

Cuts at:      0    1152    1743 
   Size:       1152     591
 
PasI CC'CwG_GG

Cuts at:      0    1325    1743 
   Size:       1325     418
 
PciI A'CATG_T

Cuts at:      0    1148    1743 
   Size:       1148     595
 
PfoI T'CCnGG_A

Cuts at:      0    1004    1265    1743 
   Size:       1004     261     478

  Fragments arranged by size: 

                1004     478     261
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     406     438    1743 
   Size:        406      32    1305

  Fragments arranged by size: 

                1305     406      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     735     836    1743 
   Size:        735     101     907

  Fragments arranged by size: 

                 907     735     101
 
PshAI GACnn'nnGTC

Cuts at:      0     841    1586    1743 
   Size:        841     745     157

  Fragments arranged by size: 

                 841     745     157
 
PsiI TTA'TAA

Cuts at:      0     202    1743 
   Size:        202    1541
 
PstI C_TGCA'G

Cuts at:      0     375    1743 
   Size:        375    1368
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1112    1743 
   Size:       1112     631
 
SfcI C'TryA_G

Cuts at:      0     371    1743 
   Size:        371    1372
 
SmlI C'TyrA_G

Cuts at:      0     901    1433    1743 
   Size:        901     532     310

  Fragments arranged by size: 

                 901     532     310
 
SpeI A'CTAG_T

Cuts at:      0     823    1743 
   Size:        823     920
 
StyI C'CwwG_G

Cuts at:      0     401     731    1743 
   Size:        401     330    1012

  Fragments arranged by size: 

                1012     401     330
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      64    1743 
   Size:         64    1679
 
TatI w'GTAC_w

Cuts at:      0     622    1314    1743 
   Size:        622     692     429

  Fragments arranged by size: 

                 692     622     429
 
XmnI GAAnn'nnTTC

Cuts at:      0      67    1207    1743 
   Size:         67    1140     536

  Fragments arranged by size: 

                1140     536      67
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI
    BamHI     BanI     BbsI     BclI    BglII Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BsrBI    BsrDI    BsrGI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtsI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI    Hin4I   HincII     MfeI     MscI     MslI   MspA1I
     NspI     PasI     PciI     PfoI     PpiI    PpuMI    PshAI     PsiI
     PstI     SapI     SfcI     SmlI     SpeI     StyI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrFI   BssHII    BssSI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PmlI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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