Restriction map for pF1KE0545
 (Linear) (Six-base) MAPSORT of: KIEE0545.seq  Check: 4487  from: 1  to: 1131



KIEE0545.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      69    1131 
   Size:         69    1062
 
AccI GT'mk_AC

Cuts at:      0     500    1131 
   Size:        500     631
 
AfeI AGC'GCT

Cuts at:      0     890    1131 
   Size:        890     241
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      54     217    1131 
   Size:         54     163     914

  Fragments arranged by size: 

                 914     163      54
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1093    1125    1131 
   Size:       1093      32       6

  Fragments arranged by size: 

                1093      32       6
 
ApaLI G'TGCA_C

Cuts at:      0     565    1131 
   Size:        565     566
 
AvaI C'yCGr_G

Cuts at:      0     122     274    1131 
   Size:        122     152     857

  Fragments arranged by size: 

                 857     152     122
 
BanI G'GyrC_C

Cuts at:      0     166     238    1131 
   Size:        166      72     893

  Fragments arranged by size: 

                 893     166      72
 
BanII G_rGCy'C

Cuts at:      0     121    1131 
   Size:        121    1010
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     809    1131 
   Size:        809     322
 
BbvCI CC'TCA_GC

Cuts at:      0     610    1131 
   Size:        610     521
 
BclI T'GATC_A

Cuts at:      0     575    1131 
   Size:        575     556
 
BglI GCCn_nnn'nGGC

Cuts at:      0     412    1131 
   Size:        412     719
 
BlpI GC'TnA_GC

Cuts at:      0     962    1131 
   Size:        962     169
 
Bme1580I G_kGCm'C

Cuts at:      0     169     569    1131 
   Size:        169     400     562

  Fragments arranged by size: 

                 562     400     169
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     663    1131 
   Size:        663     468
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     798    1131 
   Size:        445     353     333

  Fragments arranged by size: 

                 445     353     333
 
Bpu10I CC'TnA_GC

Cuts at:      0     140     610    1131 
   Size:        140     470     521

  Fragments arranged by size: 

                 521     470     140
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     730    1131 
   Size:        472     258     401

  Fragments arranged by size: 

                 472     401     258
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     289    1131 
   Size:        289     842
 
BsaAI yAC'GTr

Cuts at:      0     177    1131 
   Size:        177     954
 
BsaHI Gr'CG_yC

Cuts at:      0     266     401    1131 
   Size:        266     135     730

  Fragments arranged by size: 

                 730     266     135
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1093    1123    1131 
   Size:       1093      30       8

  Fragments arranged by size: 

                1093      30       8
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     163     307    1101    1131 
   Size:        163     144     794      30

  Fragments arranged by size: 

                 794     163     144      30
 
BseYI C'CCAG_C

Cuts at:      0      69    1131 
   Size:         69    1062
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712    1131 
   Size:        712     419
 
BsiEI CG_ry'CG

Cuts at:      0     555    1131 
   Size:        555     576
 
BsiHKAI G_wGCw'C

Cuts at:      0     464     569    1131 
   Size:        464     105     562

  Fragments arranged by size: 

                 562     464     105
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     169     464     569    1131 
   Size:        121      48     295     105     562

  Fragments arranged by size: 

                 562     295     121     105      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      69    1131 
   Size:         69    1062
 
BsrBI CCG'CTC

Cuts at:      0     207     849    1131 
   Size:        207     642     282

  Fragments arranged by size: 

                 642     282     207
 
BsrDI GCAATG_nn'

Cuts at:      0     593    1131 
   Size:        593     538
 
BsrFI r'CCGG_y

Cuts at:      0      34     450    1131 
   Size:         34     416     681

  Fragments arranged by size: 

                 681     416      34
 
BssHII G'CGCG_C

Cuts at:      0     252    1131 
   Size:        252     879
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     723    1131 
   Size:        723     408
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177     916    1131 
   Size:        177     739     215

  Fragments arranged by size: 

                 739     215     177
 
BtgI C'CryG_G

Cuts at:      0     854    1131 
   Size:        854     277
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     756    1131 
   Size:        756     375
 
BtsI GCAGTG_nn'

Cuts at:      0     240    1131 
   Size:        240     891
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     804     839    1131 
   Size:        804      35     292

  Fragments arranged by size: 

                 804     292      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     638    1131 
   Size:        638     493
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1018    1131 
   Size:       1018     113
 
EaeI y'GGCC_r

Cuts at:      0     320     348     552     906    1131 
   Size:        320      28     204     354     225

  Fragments arranged by size: 

                 354     320     225     204      28
 
EagI C'GGCC_G

Cuts at:      0     552    1131 
   Size:        552     579
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     798    1131 
   Size:        445     353     333

  Fragments arranged by size: 

                 445     353     333
 
EcoO109I rG'GnC_Cy

Cuts at:      0     474    1131 
   Size:        474     657
 
HaeII r_GCGC'y

Cuts at:      0     741     892    1131 
   Size:        741     151     239

  Fragments arranged by size: 

                 741     239     151
 
HincII GTy'rAC

Cuts at:      0     879    1131 
   Size:        879     252
 
HpaI GTT'AAC

Cuts at:      0     879    1131 
   Size:        879     252
 
MscI TGG'CCA

Cuts at:      0     322     908    1131 
   Size:        322     586     223

  Fragments arranged by size: 

                 586     322     223
 
MslI CAynn'nnrTG

Cuts at:      0     214     247     655    1131 
   Size:        214      33     408     476

  Fragments arranged by size: 

                 476     408     214      33
 
MspA1I CmG'CkG

Cuts at:      0      69     720    1038    1131 
   Size:         69     651     318      93

  Fragments arranged by size: 

                 651     318      93      69
 
NaeI GCC'GGC

Cuts at:      0      36     452    1131 
   Size:         36     416     679

  Fragments arranged by size: 

                 679     416      36
 
NgoMIV G'CCGG_C

Cuts at:      0      34     450    1131 
   Size:         34     416     681

  Fragments arranged by size: 

                 681     416      34
 
PasI CC'CwG_GG

Cuts at:      0     916    1131 
   Size:        916     215
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     675    1131 
   Size:        675     456
 
PfoI T'CCnGG_A

Cuts at:      0     422    1131 
   Size:        422     709
 
PmlI CAC'GTG

Cuts at:      0     177    1131 
   Size:        177     954
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1093    1125    1131 
   Size:       1093      32       6

  Fragments arranged by size: 

                1093      32       6
 
PpuMI rG'GwC_Cy

Cuts at:      0     474    1131 
   Size:        474     657
 
PvuII CAG'CTG

Cuts at:      0      69     720    1131 
   Size:         69     651     411

  Fragments arranged by size: 

                 651     411      69
 
SexAI A'CCwGG_T

Cuts at:      0     860    1131 
   Size:        860     271
 
SmaI CCC'GGG

Cuts at:      0     124    1131 
   Size:        124    1007
 
SmlI C'TyrA_G

Cuts at:      0     487     745    1131 
   Size:        487     258     386

  Fragments arranged by size: 

                 487     386     258
 
StuI AGG'CCT

Cuts at:      0     334     415    1131 
   Size:        334      81     716

  Fragments arranged by size: 

                 716     334      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     208     486    1131 
   Size:        208     278     645

  Fragments arranged by size: 

                 645     278     208
 
TatI w'GTAC_w

Cuts at:      0     707    1131 
   Size:        707     424
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      65      97    1131 
   Size:         65      32    1034

  Fragments arranged by size: 

                1034      65      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     787     862    1131 
   Size:        787      75     269

  Fragments arranged by size: 

                 787     269      75
 
XmaI C'CCGG_G

Cuts at:      0     122    1131 
   Size:        122    1009
 

 Enzymes that do cut:

     AarI     AccI     AfeI     AlfI     AloI    ApaLI     AvaI     BanI
    BanII     BbsI    BbvCI     BclI     BglI     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BssHII   BstAPI    BstXI     BtgI    BtgZI     BtsI    CspCI
   DraIII     DrdI     EaeI     EagI  Eco57MI EcoO109I    HaeII   HincII
     HpaI     MscI     MslI   MspA1I     NaeI   NgoMIV     PasI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PvuII    SexAI     SmaI     SmlI
     StuI    TaqII     TatI     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI    BglII    BmgBI
     BmtI     BplI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrGI    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     ClaI     DraI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I  HindIII     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     XbaI     XcmI     XhoI     XmnI     ZraI
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