Restriction map for pF1KE0165
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MslI        MspA1I      MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        TfiI        Tsp509I     Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         109  CGCG                 CG^CG
AcyI          361  GACGCC               G[GA]^CG[CT]C
AlwI          408  GGATC                GGATC(4/5)
AlwNI         187  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          374  GGGCCC               GGGCC^C
AspHI          97  GAGCAC               G[AT]GC[AT]^C
BalI           68  TGGCCA               TGG^CCA
BbsI          442  GAAGAC               GAAGAC(2/6)
BpmI          480  CTGGAG               CTGGAG(16/14)
BpuAI         442  GAAGAC               GAAGAC(2/6)
BsaHI         361  GACGCC               G[GA]^CG[CT]C
BsgI          205  CTGCAC               GTGCAG(16/14)
BsiHKAI        97  GAGCAC               G[AT]GC[AT]^C
BsmFI         171  GTCCC                GGGAC(10/14)
BsmI           82  GAATGC               GAATGC(1/-1)
BsrBI         457  GAGCGG               CCGCTC(-3/-3)
BsrI          479  ACTGG                ACTGG(1/-1)
BssSI         100  CACGAG               CACGAG(-5/-1)
BstUI         109  CGCG                 CG^CG
BstYI         408  GGATCT               [GA]^GATC[CT]
DraIII        394  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           68  TGGCCA               [CT]^GGCC[GA]
EarI           54  GAAGAG               CTCTTC(1/4)
HaeII         326  AGCGCC               [GA]GCGC^[CT]
Hin1I         361  GACGCC               G[GA]^CG[CT]C
HinfI         344  GAGTC                G^A[ACGT]TC
Ksp632I        54  GAAGAG               CTCTTC(1/4)
MaeII         491  ACGT                 A^CGT
MaeIII        476  GTGAC                ^GT[ACGT]AC
MflI          408  GGATCT               [GA]^GATC[CT]
MluNI          68  TGGCCA               TGG^CCA
MscI           68  TGGCCA               TGG^CCA
MseI          281  TTAA                 T^TAA
MvnI          109  CGCG                 CG^CG
NcoI          404  CCATGG               C^CATGG
PleI          344  GAGTC                GAGTC(4/5)
SapI           54  GAAGAGC              GCTCTTC(1/4)
SbfI          183  CCTGCAGG             CCTGCA^GG
SmlI          424  CTCAAG               C^T[CT][GA]AG
Sse8387I      183  CCTGCAGG             CCTGCA^GG
Tru9I         281  TTAA                 T^TAA
Tsp45I        476  GTGAC                ^GT[GC]AC
XhoII         408  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AluI          203  AGCT                 AG^CT
              293  AGCT                
AvaII         189  GGACC                G^G[AT]CC
              285  GGACC               
BanII         374  GGGCCC               G[GA]GC[CT]^C
              384  GGGCTC              
BbvI          153  GCTGC                GCAGC(8/12)
              204  GCTGC               
BcnI          198  CCGGG                CC^[GC]GG
              372  CCGGG               
BseRI         103  GAGGAG               GAGGAG(10/8)
              232  GAGGAG              
BsmAI         352  GTCTC                GTCTC(1/5)
              546  GTCTC               
BstF5I        144  CATCC                GGATG(2/0)
              228  GGATG               
BstNI         132  CCTGG                CC^[AT]GG
              272  CCAGG               
DdeI          116  CTGAG                C^T[ACGT]AG
              175  CTCAG               
DpnII          27  GATC                 ^GATC
              409  GATC                
EcoRII        132  CCTGG                ^CC[AT]GG
              272  CCAGG               
FokI          144  CATCC                GGATG(9/13)
              228  GGATG               
HgaI          361  GACGC                GACGC(5/10)
              339  GCGTC               
HphI          300  GGTGA                GGTGA(8/7)
              475  GGTGA               
MboI           27  GATC                 ^GATC
              409  GATC                
MvaI          132  CCTGG                CC^[AT]GG
              272  CCAGG               
MwoI           99  GCACGAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              509  GCATGGAAGGC         
NciI          198  CCGGG                CC^[GC]GG
              372  CCGGG               
NdeII          27  GATC                 ^GATC
              409  GATC                
NlaIV         374  GGGCCC               GG[ACGT]^[ACGT]CC
              385  GGCTCC              
PpuMI         188  AGGACCT              [GA]G^G[AT]CC[CT]
              284  AGGACCT             
PstI          154  CTGCAG               CTGCA^G
              184  CTGCAG              
Sau3AI         27  GATC                 ^GATC
              409  GATC                
SfcI          154  CTGCAG               C^T[GA][CT]AG
              184  CTGCAG              
TaqI           11  TCGA                 T^CGA
              342  TCGA                
TseI          153  GCTGC                G^C[AT]GC
              204  GCTGC               
TspRI          30  CACTG                CA[GC]TG[ACGT][ACGT]^
              177  CAGTG               
TthHB8I        11  TCGA                 T^CGA
              342  TCGA                
XcmI          212  CCAAGGTGGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              500  CCATGTCTGGCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       Cac8I       CfoI        Cfr13I      
DraII       DsaI        EcoO109I    EcoT14I     FauI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboII       MnlI        MspI        
NlaIII      Sau96I      ScrFI       SfaNI       StyI        
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