Restriction map for pF1KE0165
 (Linear) (Six-base) MAPSORT of: KIEE0165.seq  Check: 8940  from: 1  to: 561



KIEE0165.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     310     316     561 
   Size:         73     237       6     245

  Fragments arranged by size: 

                 245     237      73       6
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      64     561 
   Size:         64     497
 
AlwNI CAG_nnn'CTG

Cuts at:      0     192     561 
   Size:        192     369
 
ApaI G_GGCC'C

Cuts at:      0     378     561 
   Size:        378     183
 
BanII G_rGCy'C

Cuts at:      0     378     388     561 
   Size:        378      10     173

  Fragments arranged by size: 

                 378     173      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     449     561 
   Size:        449     112
 
Bme1580I G_kGCm'C

Cuts at:      0     183     378     561 
   Size:        183     195     183

  Fragments arranged by size: 

                 195     183     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     501     561 
   Size:        501      60
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     561 
   Size:        409     152
 
BsaHI Gr'CG_yC

Cuts at:      0     362     561 
   Size:        362     199
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     487     517     561 
   Size:        487      30      44

  Fragments arranged by size: 

                 487      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     247     561 
   Size:        118     129     314

  Fragments arranged by size: 

                 314     129     118
 
BseYI C'CCAG_C

Cuts at:      0      86     381     561 
   Size:         86     295     180

  Fragments arranged by size: 

                 295     180      86
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     561 
   Size:        190     371
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     561 
   Size:        101     460
 
BsmI GAATG_Cn'

Cuts at:      0      88     561 
   Size:         88     473
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     183     378     388     561 
   Size:        101      82     195      10     173

  Fragments arranged by size: 

                 195     173     101      82      10
 
BsrBI CCG'CTC

Cuts at:      0     459     561 
   Size:        459     102
 
BssSI C'ACGA_G

Cuts at:      0     100     561 
   Size:        100     461
 
BstYI r'GATC_y

Cuts at:      0     408     561 
   Size:        408     153
 
BtgI C'CryG_G

Cuts at:      0     162     312     404     561 
   Size:        162     150      92     157

  Fragments arranged by size: 

                 162     157     150      92
 
BtsI GCAGTG_nn'

Cuts at:      0      29     561 
   Size:         29     532
 
DraIII CAC_nnn'GTG

Cuts at:      0     399     561 
   Size:        399     162
 
EaeI y'GGCC_r

Cuts at:      0      68     561 
   Size:         68     493
 
EarI CTCTTCn'nnn_

Cuts at:      0      49     561 
   Size:         49     512
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     310     316     501     561 
   Size:         73     237       6     185      60

  Fragments arranged by size: 

                 237     185      73      60       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     189     285     375     561 
   Size:        189      96      90     186

  Fragments arranged by size: 

                 189     186      96      90
 
HaeII r_GCGC'y

Cuts at:      0     330     561 
   Size:        330     231
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     335     367     487     519     561 
   Size:        335      32     120      32      42

  Fragments arranged by size: 

                 335     120      42      32      32
 
MscI TGG'CCA

Cuts at:      0      70     561 
   Size:         70     491
 
NcoI C'CATG_G

Cuts at:      0     404     561 
   Size:        404     157
 
PpuMI rG'GwC_Cy

Cuts at:      0     189     285     561 
   Size:        189      96     276

  Fragments arranged by size: 

                 276     189      96
 
PspOMI G'GGCC_C

Cuts at:      0     374     561 
   Size:        374     187
 
PstI C_TGCA'G

Cuts at:      0     158     188     561 
   Size:        158      30     373

  Fragments arranged by size: 

                 373     158      30
 
SapI GCTCTTCn'nnn_

Cuts at:      0      49     561 
   Size:         49     512
 
SbfI CC_TGCA'GG

Cuts at:      0     188     561 
   Size:        188     373
 
SfcI C'TryA_G

Cuts at:      0     154     184     561 
   Size:        154      30     377

  Fragments arranged by size: 

                 377     154      30
 
SmlI C'TyrA_G

Cuts at:      0     424     561 
   Size:        424     137
 
StyI C'CwwG_G

Cuts at:      0      71      77     212     404     561 
   Size:         71       6     135     192     157

  Fragments arranged by size: 

                 192     157     135      71       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     463     561 
   Size:        463      98
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     385     417     561 
   Size:        385      32     144

  Fragments arranged by size: 

                 385     144      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     219     507     561 
   Size:        219     288      54

  Fragments arranged by size: 

                 288     219      54
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    BanII     BbsI Bme1580I     BpmI
    BpuEI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BsrBI    BssSI    BstYI     BtgI     BtsI   DraIII     EaeI
     EarI  Eco57MI EcoO109I    HaeII    Hin4I     MscI     NcoI    PpuMI
   PspOMI     PstI     SapI     SbfI     SfcI     SmlI     StyI    TaqII
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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