Restriction map for
pF1KE0196
(Circular) (Six-base) MAPSORT of: pF1KE0196.seq Check: 4899 from: 1 to: 4361
pF1KE0196 4361 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 749 749
Size: 4361
AccI GT'mk_AC
Cuts at: 1399 1399
Size: 4361
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 4201 4201
Size: 4361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 959 1004 2186 2618 3365 3633 959
Size: 45 1182 432 747 268 1687
Fragments arranged by size:
1687 1182 747 432 268 45
AflIII A'CryG_T
Cuts at: 1713 2817 3794 1713
Size: 1104 977 2280
Fragments arranged by size:
2280 1104 977
AgeI A'CCGG_T
Cuts at: 2749 2749
Size: 4361
AleI CACnn'nnGTG
Cuts at: 734 734
Size: 4361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 798 798
Size: 4361
AlwNI CAG_nnn'CTG
Cuts at: 556 1666 3233 556
Size: 1110 1567 1684
Fragments arranged by size:
1684 1567 1110
ApaLI G'TGCA_C
Cuts at: 3131 3131
Size: 4361
ApoI r'AATT_y
Cuts at: 366 454 1106 1365 3714 3970 366
Size: 88 652 259 2349 256 757
Fragments arranged by size:
2349 757 652 259 256 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 1310 1381 1310
Size: 71 4290
BamHI G'GATC_C
Cuts at: 1386 1386
Size: 4361
BanI G'GyrC_C
Cuts at: 1377 1791 2039 2074 1377
Size: 414 248 35 3664
Fragments arranged by size:
3664 414 248 35
BanII G_rGCy'C
Cuts at: 1375 2405 1375
Size: 1030 3331
BbeI G_GCGC'C
Cuts at: 1795 2043 1795
Size: 248 4113
BbsI GAAGACnn'nnnn_
Cuts at: 199 241 970 199
Size: 42 729 3590
Fragments arranged by size:
3590 729 42
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3020 4361 2259
Size: 761 1341 2259
Fragments arranged by size:
2259 1341 761
BclI T'GATC_A
Cuts at: 749 1881 749
Size: 1132 3229
BfrBI ATG'CAT
Cuts at: 358 1126 1189 358
Size: 768 63 3530
Fragments arranged by size:
3530 768 63
BglII A'GATC_T
Cuts at: 136 1876 3913 136
Size: 1740 2037 584
Fragments arranged by size:
2037 1740 584
BlpI GC'TnA_GC
Cuts at: 1473 1473
Size: 4361
Bme1580I G_kGCm'C
Cuts at: 1986 2079 3135 1986
Size: 93 1056 3212
Fragments arranged by size:
3212 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1573 1654 1989 4133 1573
Size: 81 335 2144 1801
Fragments arranged by size:
2144 1801 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 452 703 290
Size: 162 251 3948
Fragments arranged by size:
3948 251 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1537 2416 2908 3206 1537
Size: 879 492 298 2692
Fragments arranged by size:
2692 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4065 4065
Size: 4361
BsaAI yAC'GTr
Cuts at: 1714 1714
Size: 4361
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 1792 2040 2742 4238 1792
Size: 248 702 1496 1915
Fragments arranged by size:
1915 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1550 2071 2749 3023 3170 3581 1550
Size: 521 678 274 147 411 2330
Fragments arranged by size:
2330 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 821 851 821
Size: 30 4331
BseYI C'CCAG_C
Cuts at: 1265 1786 2544 3121 3617 1265
Size: 521 758 577 496 2009
Fragments arranged by size:
2009 758 577 521 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 949 460
Size: 489 3872
BsiEI CG_ry'CG
Cuts at: 104 1903 1949 3157 104
Size: 1799 46 1208 1308
Fragments arranged by size:
1799 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1375 2153 2343 3135 1375
Size: 778 190 792 2601
Fragments arranged by size:
2601 792 778 190
BsmI GAATG_Cn'
Cuts at: 552 552
Size: 4361
Bsp1286I G_dGCh'C
Cuts at: 1375 1986 2079 2153 2343 2405 3135 1375
Size: 611 93 74 190 62 730 2601
Fragments arranged by size:
2601 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3581 3581
Size: 4361
BspHI T'CATG_A
Cuts at: 882 909 3537 882
Size: 27 2628 1706
Fragments arranged by size:
2628 1706 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 749 1411 1625 1927 2308 749
Size: 662 214 302 381 2802
Fragments arranged by size:
2802 662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2653 2707 4191 4356 50
Size: 2603 54 1484 165 55
Fragments arranged by size:
2603 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 721 1119 2273 721
Size: 398 1154 2809
Fragments arranged by size:
2809 1154 398
BsrFI r'CCGG_y
Cuts at: 2359 2540 2749 2359
Size: 181 209 3971
Fragments arranged by size:
3971 209 181
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 4361
BssSI C'ACGA_G
Cuts at: 2632 2990 2632
Size: 358 4003
BstAPI GCAn_nnn'nTGC
Cuts at: 556 1738 556
Size: 1182 3179
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstYI r'GATC_y
Cuts at: 1 136 1386 1853 1876 2211 2457 3469 3555
Size: 135 1250 467 23 335 246 1012 86
Cuts at: 3555 3567 3913 1
Size: 12 346 449
Fragments arranged by size:
1250 1012 467 449 346 335 246 135
86 23 12
BtgI C'CryG_G
Cuts at: 697 697
Size: 4361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2311 2492 2655 2311
Size: 181 163 4017
Fragments arranged by size:
4017 181 163
BtsI GCAGTG_nn'
Cuts at: 780 1604 780
Size: 824 3537
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 600 635 600
Size: 35 4326
DraI TTT'AAA
Cuts at: 1360 1360
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 2925 2067
Size: 858 3503
EaeI y'GGCC_r
Cuts at: 142 694 1946 2120 2511 2538 3883 142
Size: 552 1252 174 391 27 1345 620
Fragments arranged by size:
1345 1252 620 552 391 174 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 2384 2594 2384
Size: 210 4151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2569 2879 3025 4171 2569
Size: 310 146 1146 2759
Fragments arranged by size:
2759 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1373 1373
Size: 4361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 452 703 959 1004 2186 2618 3365 3633
Size: 162 251 256 45 1182 432 747 268
Cuts at: 3633 290
Size: 1018
Fragments arranged by size:
1182 1018 747 432 268 256 251 162
45
EcoO109I rG'GnC_Cy
Cuts at: 1500 1500
Size: 4361
EcoRI G'AATT_C
Cuts at: 1365 1365
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 1795 2043 3065 4044 1795
Size: 248 1022 979 2112
Fragments arranged by size:
2112 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1308 1340 2203 2233 2235 2265 1308
Size: 32 863 30 2 30 3404
Fragments arranged by size:
3404 863 32 30 30 2
HincII GTy'rAC
Cuts at: 822 1400 822
Size: 578 3783
KasI G'GCGC_C
Cuts at: 1791 2039 1791
Size: 248 4113
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MfeI C'AATT_G
Cuts at: 1329 1329
Size: 4361
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1233 3032 3216 1233
Size: 1799 184 2378
Fragments arranged by size:
2378 1799 184
MscI TGG'CCA
Cuts at: 144 696 2122 144
Size: 552 1426 2383
Fragments arranged by size:
2383 1426 552
MslI CAynn'nnrTG
Cuts at: 696 734 2350 2477 696
Size: 38 1616 127 2580
Fragments arranged by size:
2580 1616 127 38
MspA1I CmG'CkG
Cuts at: 450 1472 1786 2146 3159 3404 450
Size: 1022 314 360 1013 245 1407
Fragments arranged by size:
1407 1022 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2542 2542
Size: 4361
NarI GG'CG_CC
Cuts at: 1792 2040 1792
Size: 248 4113
NcoI C'CATG_G
Cuts at: 697 697
Size: 4361
NdeI CA'TA_TG
Cuts at: 1122 1122
Size: 4361
NgoMIV G'CCGG_C
Cuts at: 2540 2540
Size: 4361
NsiI A_TGCA'T
Cuts at: 360 1128 1191 360
Size: 768 63 3530
Fragments arranged by size:
3530 768 63
NspI r_CATG'y
Cuts at: 714 1193 1414 1914 2821 714
Size: 479 221 500 907 2254
Fragments arranged by size:
2254 907 500 479 221
PciI A'CATG_T
Cuts at: 2817 2817
Size: 4361
PfoI T'CCnGG_A
Cuts at: 541 541
Size: 4361
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 4329
PspXI vC'TCGA_Gb
Cuts at: 1310 1310
Size: 4361
PstI C_TGCA'G
Cuts at: 777 1408 2093 777
Size: 631 685 3045
Fragments arranged by size:
3045 685 631
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 450 1786 2146 450
Size: 1336 360 2665
Fragments arranged by size:
2665 1336 360
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 1375 1375
Size: 4361
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 2384 2594 2384
Size: 210 4151
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 3878 3878
Size: 4361
SfcI C'TryA_G
Cuts at: 33 773 1404 2089 3082 3273 33
Size: 740 631 685 993 191 1121
Fragments arranged by size:
1121 993 740 685 631 191
SfoI GGC'GCC
Cuts at: 1793 2041 1793
Size: 248 4113
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 1310 1516 2431 2923 3185 1310
Size: 206 915 492 262 2486
Fragments arranged by size:
2486 915 492 262 206
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 714 1414 1914 714
Size: 700 500 3161
Fragments arranged by size:
3161 700 500
StyI C'CwwG_G
Cuts at: 697 1495 1849 697
Size: 798 354 3209
Fragments arranged by size:
3209 798 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1944 2077 2536 2745 1944
Size: 133 459 209 3560
Fragments arranged by size:
3560 459 209 133
TatI w'GTAC_w
Cuts at: 110 959 3876 110
Size: 849 2917 595
Fragments arranged by size:
2917 849 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 848 880 3878 3910 848
Size: 32 2998 32 1299
Fragments arranged by size:
2998 1299 32 32
Tth111I GACn'n_nGTC
Cuts at: 2158 2158
Size: 4361
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 570 1856 570
Size: 1286 3075
XhoI C'TCGA_G
Cuts at: 1310 1310
Size: 4361
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BseRI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI