Restriction map for
pF1KE0182
(Circular) (Six-base) MAPSORT of: pF1KE0182.seq Check: 7437 from: 1 to: 3791
pF1KE0182 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 776 1616 2048 2795 3063 455
Size: 321 840 432 747 268 1183
Fragments arranged by size:
1183 840 747 432 321 268
AfeI AGC'GCT
Cuts at: 196 226 196
Size: 30 3761
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 675 754 1096 2663 675
Size: 79 342 1567 1803
Fragments arranged by size:
1803 1567 342 79
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 795 3144 3400 795
Size: 2349 256 1186
Fragments arranged by size:
2349 1186 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 369 811 369
Size: 442 3349
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 259 807 1221 1469 1504 259
Size: 548 414 248 35 2546
Fragments arranged by size:
2546 548 414 248 35
BanII G_rGCy'C
Cuts at: 284 451 805 1835 284
Size: 167 354 1030 2240
Fragments arranged by size:
2240 1030 354 167
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BbsI GAAGACnn'nnnn_
Cuts at: 356 472 356
Size: 116 3675
BbvCI CC'TCA_GC
Cuts at: 686 686
Size: 3791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 573 607 573
Size: 34 3757
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglI GCCn_nnn'nGGC
Cuts at: 749 758 749
Size: 9 3782
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 1416 1509 2565 1416
Size: 93 1056 2642
Fragments arranged by size:
2642 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 267 1003 1084 1419 3563 267
Size: 736 81 335 2144 495
Fragments arranged by size:
2144 736 495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 3791
Bpu10I CC'TnA_GC
Cuts at: 686 686
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1846 2338 2636 967
Size: 879 492 298 2122
Fragments arranged by size:
2122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 206 262 379 3495 206
Size: 56 117 3116 502
Fragments arranged by size:
3116 502 117 56
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 259 289 259
Size: 30 3761
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 703
Size: 3791
BseYI C'CCAG_C
Cuts at: 1216 1974 2551 3047 1216
Size: 758 577 496 1960
Fragments arranged by size:
1960 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 208 1333 1379 2587 104
Size: 104 1125 46 1208 1308
Fragments arranged by size:
1308 1208 1125 104 46
BsiHKAI G_wGCw'C
Cuts at: 805 1583 1773 2565 805
Size: 778 190 792 2031
Fragments arranged by size:
2031 792 778 190
BsmI GAATG_Cn'
Cuts at: 636 636
Size: 3791
Bsp1286I G_dGCh'C
Cuts at: 284 451 805 1416 1509 1583 1773 1835 2565
Size: 167 354 611 93 74 190 62 730
Cuts at: 2565 284
Size: 1510
Fragments arranged by size:
1510 730 611 354 190 167 93 74
62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 841 1055 1357 1738 224
Size: 617 214 302 381 2277
Fragments arranged by size:
2277 617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 389 1703 389
Size: 1314 2477
BsrFI r'CCGG_y
Cuts at: 203 1789 1970 2179 203
Size: 1586 181 209 1815
Fragments arranged by size:
1815 1586 209 181
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 156 444 570 156
Size: 288 126 3377
Fragments arranged by size:
3377 288 126
BstYI r'GATC_y
Cuts at: 1 816 1283 1306 1641 1887 2899 2985 2997
Size: 815 467 23 335 246 1012 86 12
Cuts at: 2997 3343 1
Size: 346 449
Fragments arranged by size:
1012 815 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 263 107
Size: 156 3635
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 384 1034 384
Size: 650 3141
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DraIII CAC_nnn'GTG
Cuts at: 615 615
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 143 205 500 1376 1550 1941 1968 3313 143
Size: 62 295 876 174 391 27 1345 621
Fragments arranged by size:
1345 876 621 391 295 174 62 27
EagI C'GGCC_G
Cuts at: 205 1376 205
Size: 1171 2620
EarI CTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 455 776 1616 2048 2795 3063 361
Size: 94 321 840 432 747 268 1089
Fragments arranged by size:
1089 840 747 432 321 268 94
EcoO109I rG'GnC_Cy
Cuts at: 751 930 751
Size: 179 3612
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3791
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 198 228 1225 1473 2495 3474 198
Size: 30 997 248 1022 979 515
Fragments arranged by size:
1022 997 979 515 248 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 534 566 1633 1663 1665 1695 259
Size: 32 243 32 1067 30 2 30 2355
Fragments arranged by size:
2355 1067 243 32 32 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
HindIII A'AGCT_T
Cuts at: 427 427
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MfeI C'AATT_G
Cuts at: 782 782
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 442 536 2462 2646 442
Size: 94 1926 184 1587
Fragments arranged by size:
1926 1587 184 94
MscI TGG'CCA
Cuts at: 145 502 1552 145
Size: 357 1050 2384
Fragments arranged by size:
2384 1050 357
MslI CAynn'nnrTG
Cuts at: 136 442 493 496 1780 1907 136
Size: 306 51 3 1284 127 2020
Fragments arranged by size:
2020 1284 306 127 51 3
MspA1I CmG'CkG
Cuts at: 902 1216 1576 2589 2834 902
Size: 314 360 1013 245 1859
Fragments arranged by size:
1859 1013 360 314 245
NaeI GCC'GGC
Cuts at: 205 1972 205
Size: 1767 2024
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 107 263 107
Size: 156 3635
NgoMIV G'CCGG_C
Cuts at: 203 1970 203
Size: 1767 2024
NspI r_CATG'y
Cuts at: 844 1344 2251 844
Size: 500 907 2384
Fragments arranged by size:
2384 907 500
PasI CC'CwG_GG
Cuts at: 518 518
Size: 3791
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PfoI T'CCnGG_A
Cuts at: 569 569
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PstI C_TGCA'G
Cuts at: 224 770 838 1523 224
Size: 546 68 685 2492
Fragments arranged by size:
2492 685 546 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 1216 1576 1216
Size: 360 3431
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
SbfI CC_TGCA'GG
Cuts at: 770 838 770
Size: 68 3723
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SexAI A'CCwGG_T
Cuts at: 611 611
Size: 3791
SfcI C'TryA_G
Cuts at: 33 220 766 834 1519 2512 2703 33
Size: 187 546 68 685 993 191 1121
Fragments arranged by size:
1121 993 685 546 191 187 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 749 749
Size: 3791
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 946 1861 2353 2615 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StuI AGG'CCT
Cuts at: 653 743 653
Size: 90 3701
StyI C'CwwG_G
Cuts at: 107 173 263 925 1279 107
Size: 66 90 662 354 2619
Fragments arranged by size:
2619 662 354 90 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 273 304 1588 273
Size: 31 1284 2476
Fragments arranged by size:
2476 1284 31
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 138 153 1286 114
Size: 24 15 1133 2619
Fragments arranged by size:
2619 1133 24 15
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
XmnI GAAnn'nnTTC
Cuts at: 704 704
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI