Restriction map for pF1KB4879
 (Linear) (Six-base) MAPSORT of: KIBB4879.seq  Check: 9675  from: 1  to: 2430



KIBB4879.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1692    2430 
   Size:       1692     738
 
AatII G_ACGT'C

Cuts at:      0     951    2102    2430 
   Size:        951    1151     328

  Fragments arranged by size: 

                1151     951     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     904    1333    1802    2430 
   Size:        149     755     429     469     628

  Fragments arranged by size: 

                 755     628     469     429     149
 
AflIII A'CryG_T

Cuts at:      0    1451    2278    2378    2430 
   Size:       1451     827     100      52

  Fragments arranged by size: 

                1451     827     100      52
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1577    2430 
   Size:       1577     853
 
AlwNI CAG_nnn'CTG

Cuts at:      0     127     156     403    1642    2430 
   Size:        127      29     247    1239     788

  Fragments arranged by size: 

                1239     788     247     127      29
 
ApoI r'AATT_y

Cuts at:      0      36     349     840    1495    1516    2430 
   Size:         36     313     491     655      21     914

  Fragments arranged by size: 

                 914     655     491     313      36      21
 
AvrII C'CTAG_G

Cuts at:      0    1236    2430 
   Size:       1236    1194
 
BamHI G'GATC_C

Cuts at:      0    1510    2430 
   Size:       1510     920
 
BanII G_rGCy'C

Cuts at:      0    1829    2430 
   Size:       1829     601
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     197     749     899     910    1802    2041    2430 
   Size:        197     552     150      11     892     239     389

  Fragments arranged by size: 

                 892     552     389     239     197     150      11
 
BbvCI CC'TCA_GC

Cuts at:      0      98    2430 
   Size:         98    2332
 
BclI T'GATC_A

Cuts at:      0    2300    2328    2430 
   Size:       2300      28     102

  Fragments arranged by size: 

                2300     102      28
 
BlpI GC'TnA_GC

Cuts at:      0    1817    2430 
   Size:       1817     613
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1018    1397    2430 
   Size:       1018     379    1033

  Fragments arranged by size: 

                1033    1018     379
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1118    1379    2430 
   Size:       1118     261    1051

  Fragments arranged by size: 

                1118    1051     261
 
Bpu10I CC'TnA_GC

Cuts at:      0      98     473     689     972    1139    1808    2430 
   Size:         98     375     216     283     167     669     622

  Fragments arranged by size: 

                 669     622     375     283     216     167      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1180    1523    2430 
   Size:       1180     343     907

  Fragments arranged by size: 

                1180     907     343
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2113    2209    2404    2430 
   Size:       2113      96     195      26

  Fragments arranged by size: 

                2113     195      96      26
 
BsaAI yAC'GTr

Cuts at:      0    2281    2430 
   Size:       2281     149
 
BsaBI GATnn'nnATC

Cuts at:      0    1467    2430 
   Size:       1467     963
 
BsaHI Gr'CG_yC

Cuts at:      0     948    1615    1718    2099    2430 
   Size:        948     667     103     381     331

  Fragments arranged by size: 

                 948     667     381     331     103
 
BsaWI w'CCGG_w

Cuts at:      0     408    2430 
   Size:        408    2022
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     240    1879    2430 
   Size:        240    1639     551

  Fragments arranged by size: 

                1639     551     240
 
BseYI C'CCAG_C

Cuts at:      0     876    2430 
   Size:        876    1554
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476    1664    2430 
   Size:        476    1188     766

  Fragments arranged by size: 

                1188     766     476
 
BsiHKAI G_wGCw'C

Cuts at:      0     538    1566    2430 
   Size:        538    1028     864

  Fragments arranged by size: 

                1028     864     538
 
BsmI GAATG_Cn'

Cuts at:      0    1170    2430 
   Size:       1170    1260
 
Bsp1286I G_dGCh'C

Cuts at:      0     538    1566    1829    2430 
   Size:        538    1028     263     601

  Fragments arranged by size: 

                1028     601     538     263
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     810    1112    1692    2430 
   Size:        810     302     580     738

  Fragments arranged by size: 

                 810     738     580     302
 
BsrBI CCG'CTC

Cuts at:      0     213    2430 
   Size:        213    2217
 
BsrGI T'GTAC_A

Cuts at:      0    2340    2430 
   Size:       2340      90
 
BstYI r'GATC_y

Cuts at:      0     435     492    1510    2430 
   Size:        435      57    1018     920

  Fragments arranged by size: 

                1018     920     435      57
 
BtgI C'CryG_G

Cuts at:      0    1032    1933    2430 
   Size:       1032     901     497

  Fragments arranged by size: 

                1032     901     497
 
BtsI GCAGTG_nn'

Cuts at:      0     459     630    2430 
   Size:        459     171    1800

  Fragments arranged by size: 

                1800     459     171
 
DraI TTT'AAA

Cuts at:      0     383    1059    2430 
   Size:        383     676    1371

  Fragments arranged by size: 

                1371     676     383
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2182    2430 
   Size:       2182     248
 
EaeI y'GGCC_r

Cuts at:      0    1530    2430 
   Size:       1530     900
 
EarI CTCTTCn'nnn_

Cuts at:      0       7      53     365     524    1085    2430 
   Size:          7      46     312     159     561    1345

  Fragments arranged by size: 

                1345     561     312     159      46       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     789    2430 
   Size:        789    1641
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     904    1118    1333    1379    1802    2430 
   Size:        149     755     214     215      46     423     628

  Fragments arranged by size: 

                 755     628     423     215     214     149      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     387    2020    2334    2430 
   Size:        387    1633     314      96

  Fragments arranged by size: 

                1633     387     314      96
 
EcoRV GAT'ATC

Cuts at:      0    1230    2430 
   Size:       1230    1200
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     721    2430 
   Size:        721    1709
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234    1122    1154    1214    1246    1527    1559 
   Size:        202      32     888      32      60      32     281      32

Cuts at:   1559    2430 
   Size:        871

  Fragments arranged by size: 

                 888     871     281     202      60      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     887    2430 
   Size:        887    1543
 
HindIII A'AGCT_T

Cuts at:      0     161     593    2430 
   Size:        161     432    1837

  Fragments arranged by size: 

                1837     432     161
 
HpaI GTT'AAC

Cuts at:      0     887    2430 
   Size:        887    1543
 
MfeI C'AATT_G

Cuts at:      0     392    2430 
   Size:        392    2038
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     629    2430 
   Size:        629    1801
 
MslI CAynn'nnrTG

Cuts at:      0    2383    2430 
   Size:       2383      47
 
MspA1I CmG'CkG

Cuts at:      0     127    1642    2430 
   Size:        127    1515     788

  Fragments arranged by size: 

                1515     788     127
 
NcoI C'CATG_G

Cuts at:      0    1032    2430 
   Size:       1032    1398
 
NspI r_CATG'y

Cuts at:      0     630     688    2382    2430 
   Size:        630      58    1694      48

  Fragments arranged by size: 

                1694     630      58      48
 
PciI A'CATG_T

Cuts at:      0    2378    2430 
   Size:       2378      52
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1038    2430 
   Size:       1038    1392
 
PfoI T'CCnGG_A

Cuts at:      0    1618    2430 
   Size:       1618     812
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     398     430    2430 
   Size:        398      32    2000

  Fragments arranged by size: 

                2000     398      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2020    2334    2430 
   Size:       2020     314      96

  Fragments arranged by size: 

                2020     314      96
 
PshAI GACnn'nnGTC

Cuts at:      0    1661    1856    2430 
   Size:       1661     195     574

  Fragments arranged by size: 

                1661     574     195
 
PsiI TTA'TAA

Cuts at:      0    1325    2430 
   Size:       1325    1105
 
PvuII CAG'CTG

Cuts at:      0     127    1642    2430 
   Size:        127    1515     788

  Fragments arranged by size: 

                1515     788     127
 
SfcI C'TryA_G

Cuts at:      0    1472    2430 
   Size:       1472     958
 
SmlI C'TyrA_G

Cuts at:      0    1159    1538    2430 
   Size:       1159     379     892

  Fragments arranged by size: 

                1159     892     379
 
StuI AGG'CCT

Cuts at:      0    1578    2430 
   Size:       1578     852
 
StyI C'CwwG_G

Cuts at:      0     835    1032    1079    1236    1297    2390    2430 
   Size:        835     197      47     157      61    1093      40

  Fragments arranged by size: 

                1093     835     197     157      61      47      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1673    2387    2428    2430 
   Size:       1673     714      41       2

  Fragments arranged by size: 

                1673     714      41       2
 
TatI w'GTAC_w

Cuts at:      0    2340    2430 
   Size:       2340      90
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1422    1454    2430 
   Size:       1422      32     976

  Fragments arranged by size: 

                1422     976      32
 
ZraI GAC'GTC

Cuts at:      0     949    2100    2430 
   Size:        949    1151     330

  Fragments arranged by size: 

                1151     949     330
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AlfI    AlwNI     ApoI    AvrII
    BamHI    BanII     BbsI    BbvCI     BclI     BlpI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrGI
    BstYI     BtgI     BtsI     DraI     DrdI     EaeI     EarI     EciI
  Eco57MI EcoO109I    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI
     MfeI     MmeI     MslI   MspA1I     NcoI     NspI     PciI    PflMI
     PfoI     PpiI    PpuMI    PshAI     PsiI    PvuII     SfcI     SmlI
     StuI     StyI    TaqII     TatI     TstI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI
     BanI     BbeI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EagI
  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI    HaeII     KasI
     KpnI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
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