Restriction map for pF1KE0522
##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AciI          184  GCGG                 CCGC(-3/-1)
AcsI          411  AAATTC               [GA]^AATT[CT]
AfaI          243  GTAC                 GT^AC
AlwNI         263  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          411  AAATTC               [GA]^AATT[CT]
AvaII         265  GGACC                G^G[AT]CC
BpmI          246  CTCCAG               CTGGAG(16/14)
BsmAI         313  GTCTC                GTCTC(1/5)
BspMI         267  ACCTGC               ACCTGC(4/8)
BsrDI         323  GCAATG               GCAATG(2/0)
BsrI          136  ACTGG                ACTGG(1/-1)
BstAPI        262  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        116  CATCC                GGATG(2/0)
BstYI         360  GGATCT               [GA]^GATC[CT]
Cac8I         222  GCTAGC               GC[ACGT]^[ACGT]GC
Cfr13I        265  GGACC                G^G[ACGT]CC
DraI          157  TTTAAA               TTT^AAA
DraII         264  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI           62  CCATGG               C^C[GA][CT]GG
EcoO109I      264  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I        62  CCATGG               C^C[AT][AT]GG
FokI          116  CATCC                GGATG(9/13)
MflI          360  GGATCT               [GA]^GATC[CT]
MwoI          262  GCAGGACCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI           62  CCATGG               C^CATGG
NheI          222  GCTAGC               G^CTAGC
PpuMI         264  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          217  CTGCAG               CTGCA^G
RsaI          243  GTAC                 GT^AC
Sau96I        265  GGACC                G^G[ACGT]CC
SfaNI         115  GCATC                GCATC(5/9)
SmlI          196  CTTGAG               C^T[CT][GA]AG
StyI           62  CCATGG               C^C[AT][AT]GG
TaqI          229  TCGA                 T^CGA
Tsp45I        256  GTGAC                ^GT[GC]AC
TthHB8I       229  TCGA                 T^CGA
XhoII         360  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AlwI          190  GGATC                GGATC(4/5)
              360  GGATC               
BbvI          219  GCAGC                GCAGC(8/12)
              216  GCTGC               
DdeI          179  CTAAG                C^T[ACGT]AG
              382  CTTAG               
DpnII         191  GATC                 ^GATC
              361  GATC                
Fnu4HI        216  GCTGC                GC^[ACGT]GC
              219  GCAGC               
HphI          255  GGTGA                GGTGA(8/7)
              316  TCACC               
ItaI          216  GCTGC                GC^[ACGT]GC
              219  GCAGC               
MboI          191  GATC                 ^GATC
              361  GATC                
MboII         339  TCTTC                GAAGA(8/7)
              342  TCTTC               
MnlI          272  CCTC                 CCTC(7/6)
              175  GAGG                
NdeII         191  GATC                 ^GATC
              361  GATC                
NlaIII         63  CATG                 CATG^
              370  CATG                
Sau3AI        191  GATC                 ^GATC
              361  GATC                
SfcI          217  CTGCAG               C^T[GA][CT]AG
              308  CTACAG              
TseI          216  GCTGC                G^C[AT]GC
              219  GCAGC               
TspRI          12  CACTG                CA[GC]TG[ACGT][ACGT]^
              239  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AluI        BfaI        BsaJI       BssKI       BstNI       
EcoRII      MaeI        MaeIII      MseI        MvaI        
ScrFI       Tru9I       Tsp509I     
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