Restriction map for pF1KF0021
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AvrII       BalI        BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsiEI       BsiHKAI     BsiWI       BspDI       BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      CelII       
ClaI        DraI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        KasI        KpnI        MaeI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TaqI        TfiI        TthHB8I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          841  GTCTAC               GT^[AC][GT]AC
AccIII        818  TCCGGA               T^CCGGA
AgeI          587  ACCGGT               A^CCGGT
AhdI         1249  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         700  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          598  GGGCCC               GGGCC^C
AspEI        1249  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1186  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          810  CTCGGG               C^[CT]CG[GA]G
AviII        1127  TGCGCA               TGC^GCA
BanII         598  GGGCCC               G[GA]GC[CT]^C
BbvCI        1470  CCTCAGC              CCTCAGC(-5/-2)
BpmI         1252  CTGGAG               CTGGAG(16/14)
BsaBI         759  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         818  TCCGGA               T^CCGGA
BsmI          106  GCATTC               GAATGC(1/-1)
BsoBI         810  CTCGGG               C^[CT]CG[GA]G
BspEI         818  TCCGGA               T^CCGGA
BsrBI         313  GAGCGG               CCGCTC(-3/-3)
BssHII       1027  GCGCGC               G^CGCGC
BstAPI        861  GCACTCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        519  GGTGACC              G^GT[ACGT]ACC
BstPI         519  GGTGACC              G^GT[ACGT]ACC
BstXI        1205  CCATCACCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI          657  CGGACCG              CG^G[AT]CCG
Eam1105I     1249  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       519  GGTGACC              G^GT[ACGT]ACC
FspI         1127  TGCGCA               TGC^GCA
HincII        433  GTCAAC               GT[CT]^[GA]AC
HindII        433  GTCAAC               GT[CT]^[GA]AC
KspI          987  CCGCGG               CCGC^GG
MamI          759  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          818  TCCGGA               T^CCGGA
MseI           65  TTAA                 T^TAA
NcoI          197  CCATGG               C^CATGG
PflFI        1186  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1245  CCACGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         587  ACCGGT               A^CCGGT
PpuMI        1384  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII        1348  CAGCTG               CAG^CTG
RsrII         657  CGGACCG              CG^G[AT]CCG
SacII         987  CCGCGG               CCGC^GG
SmlI          244  CTCAAG               C^T[CT][GA]AG
Tru9I          65  TTAA                 T^TAA
Tsp509I        12  AATT                 ^AATT
Tth111I      1186  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1245  CCACGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AatII         676  GACGTC               GACGT^C
             1036  GACGTC              
AflIII         59  ACATGT               A^C[GA][CT]GT
             1007  ACATGT              
BsaAI         931  TACGTG               [CT]AC^GT[GA]
             1167  CACGTA              
BsaWI         587  ACCGGT               [AT]^CCGG[AT]
              818  TCCGGA              
BsgI         1239  CTGCAC               GTGCAG(16/14)
              343  GTGCAG              
BsmBI         888  CGTCTC               CGTCTC(1/5)
              348  GAGACG              
Bsp1407I       56  TGTACA               T^GTACA
             1010  TGTACA              
BspHI         161  TCATGA               T^CATGA
              740  TCATGA              
BspLU11I       59  ACATGT               A^CATGT
             1007  ACATGT              
BsrDI        1113  CATTGC               GCAATG(2/0)
              539  GCAATG              
BsrFI         587  ACCGGT               [GA]^CCGG[CT]
             1291  ACCGGC              
BsrGI          56  TGTACA               T^GTACA
             1010  TGTACA              
BstYI         355  GGATCT               [GA]^GATC[CT]
              701  AGATCC              
Cfr10I        587  ACCGGT               [GA]^CCGG[CT]
             1291  ACCGGC              
DdeI          279  CTCAG                C^T[ACGT]AG
             1471  CTCAG               
EaeI         1049  TGGCCG               [CT]^GGCC[GA]
             1457  CGGCCA              
EcoNI         243  CCTCAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              510  CCTCATGCAGG         
EcoT14I       101  CCAAGG               C^C[AT][AT]GG
              197  CCATGG              
HaeII         529  AGCGCC               [GA]GCGC^[CT]
              899  AGCGCC              
MflI          355  GGATCT               [GA]^GATC[CT]
              701  AGATCC              
NspI           59  ACATGT               [GA]CATG^[CT]
             1007  ACATGT              
RcaI          161  TCATGA               T^CATGA
              740  TCATGA              
SspBI          56  TGTACA               T^GTACA
             1010  TGTACA              
StyI          101  CCAAGG               C^C[AT][AT]GG
              197  CCATGG              
XcmI         1202  CCACCATCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1347  CCAGCTGGCCTTTGG     
XhoII         355  GGATCT               [GA]^GATC[CT]
              701  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaII       BanI        BbsI        BbvI        
BcnI        BmyI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
Tsp45I      TspRI       
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