Restriction map for pF1KF0021
 (Linear) (Six-base) MAPSORT of: FLJ00021.seq  Check: 8982  from: 1  to: 1488



FLJ00021.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     507    1488 
   Size:        507     981
 
AatII G_ACGT'C

Cuts at:      0     680    1040    1488 
   Size:        680     360     448

  Fragments arranged by size: 

                 680     448     360
 
AccI GT'mk_AC

Cuts at:      0     842    1488 
   Size:        842     646
 
AflIII A'CryG_T

Cuts at:      0      59    1007    1488 
   Size:         59     948     481

  Fragments arranged by size: 

                 948     481      59
 
AgeI A'CCGG_T

Cuts at:      0     587    1488 
   Size:        587     901
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1254    1488 
   Size:       1254     234
 
AleI CACnn'nnGTG

Cuts at:      0     184    1213    1488 
   Size:        184    1029     275

  Fragments arranged by size: 

                1029     275     184
 
AlwNI CAG_nnn'CTG

Cuts at:      0     705    1488 
   Size:        705     783
 
ApaI G_GGCC'C

Cuts at:      0     602    1488 
   Size:        602     886
 
AvaI C'yCGr_G

Cuts at:      0     810    1488 
   Size:        810     678
 
BanI G'GyrC_C

Cuts at:      0     788    1215    1288    1488 
   Size:        788     427      73     200

  Fragments arranged by size: 

                 788     427     200      73
 
BanII G_rGCy'C

Cuts at:      0     602    1488 
   Size:        602     886
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     691    1077    1147    1488 
   Size:        691     386      70     341

  Fragments arranged by size: 

                 691     386     341      70
 
BbvCI CC'TCA_GC

Cuts at:      0    1471    1488 
   Size:       1471      17
 
Bme1580I G_kGCm'C

Cuts at:      0      82     503     602     773     791    1291    1488 
   Size:         82     421      99     171      18     500     197

  Fragments arranged by size: 

                 500     421     197     171      99      82      18
 
BmgBI CAC'GTC

Cuts at:      0     352    1488 
   Size:        352    1136
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1273    1488 
   Size:       1273     215
 
Bpu10I CC'TnA_GC

Cuts at:      0    1471    1488 
   Size:       1471      17
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229    1488 
   Size:        229    1259
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      13     467    1471    1488 
   Size:         13     454    1004      17

  Fragments arranged by size: 

                1004     454      17      13
 
BsaAI yAC'GTr

Cuts at:      0     933    1169    1488 
   Size:        933     236     319

  Fragments arranged by size: 

                 933     319     236
 
BsaBI GATnn'nnATC

Cuts at:      0     763    1488 
   Size:        763     725
 
BsaHI Gr'CG_yC

Cuts at:      0     617     677    1037    1488 
   Size:        617      60     360     451

  Fragments arranged by size: 

                 617     451     360      60
 
BsaWI w'CCGG_w

Cuts at:      0     587     818    1488 
   Size:        587     231     670

  Fragments arranged by size: 

                 670     587     231
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     253     283    1488 
   Size:        253      30    1205

  Fragments arranged by size: 

                1205     253      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     258     265     640     643     882     949    1488 
   Size:        258       7     375       3     239      67     539

  Fragments arranged by size: 

                 539     375     258     239      67       7       3
 
BseYI C'CCAG_C

Cuts at:      0     593    1346    1488 
   Size:        593     753     142

  Fragments arranged by size: 

                 753     593     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1224    1488 
   Size:        364     860     264

  Fragments arranged by size: 

                 860     364     264
 
BsmI GAATG_Cn'

Cuts at:      0     106    1488 
   Size:        106    1382
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     342     894    1488 
   Size:        342     552     594

  Fragments arranged by size: 

                 594     552     342
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     503     602     773     791    1291    1488 
   Size:         82     421      99     171      18     500     197

  Fragments arranged by size: 

                 500     421     197     171      99      82      18
 
BspEI T'CCGG_A

Cuts at:      0     818    1488 
   Size:        818     670
 
BspHI T'CATG_A

Cuts at:      0     161     740    1488 
   Size:        161     579     748

  Fragments arranged by size: 

                 748     579     161
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     507     532     704    1488 
   Size:        507      25     172     784

  Fragments arranged by size: 

                 784     507     172      25
 
BsrBI CCG'CTC

Cuts at:      0     315    1488 
   Size:        315    1173
 
BsrDI GCAATG_nn'

Cuts at:      0     546    1112    1488 
   Size:        546     566     376

  Fragments arranged by size: 

                 566     546     376
 
BsrFI r'CCGG_y

Cuts at:      0     587    1291    1488 
   Size:        587     704     197

  Fragments arranged by size: 

                 704     587     197
 
BsrGI T'GTAC_A

Cuts at:      0      56    1010    1488 
   Size:         56     954     478

  Fragments arranged by size: 

                 954     478      56
 
BssHII G'CGCG_C

Cuts at:      0    1027    1488 
   Size:       1027     461
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     867    1488 
   Size:        867     621
 
BstEII G'GTnAC_C

Cuts at:      0     519    1488 
   Size:        519     969
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1212    1488 
   Size:       1212     276
 
BstYI r'GATC_y

Cuts at:      0     355     701    1488 
   Size:        355     346     787

  Fragments arranged by size: 

                 787     355     346
 
BtgI C'CryG_G

Cuts at:      0     197     987    1174    1488 
   Size:        197     790     187     314

  Fragments arranged by size: 

                 790     314     197     187
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     302    1488 
   Size:        302    1186
 
BtsI GCAGTG_nn'

Cuts at:      0     113    1329    1488 
   Size:        113    1216     159

  Fragments arranged by size: 

                1216     159     113
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     165     200    1488 
   Size:        165      35    1288

  Fragments arranged by size: 

                1288     165      35
 
EaeI y'GGCC_r

Cuts at:      0    1049    1457    1488 
   Size:       1049     408      31

  Fragments arranged by size: 

                1049     408      31
 
EarI CTCTTCn'nnn_

Cuts at:      0      42     223    1081    1153    1488 
   Size:         42     181     858      72     335

  Fragments arranged by size: 

                 858     335     181      72      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1384    1488 
   Size:       1384     104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1273    1488 
   Size:       1273     215
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     247     514    1488 
   Size:        247     267     974

  Fragments arranged by size: 

                 974     267     247
 
EcoO109I rG'GnC_Cy

Cuts at:      0     598     599    1277    1385    1488 
   Size:        598       1     678     108     103

  Fragments arranged by size: 

                 678     598     108     103       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1164    1488 
   Size:       1164     324
 
FspI TGC'GCA

Cuts at:      0    1129    1488 
   Size:       1129     359
 
HaeII r_GCGC'y

Cuts at:      0     533     903    1488 
   Size:        533     370     585

  Fragments arranged by size: 

                 585     533     370
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     250     253     282     285     630     662    1240    1272 
   Size:        250       3      29       3     345      32     578      32

Cuts at:   1272    1488 
   Size:        216

  Fragments arranged by size: 

                 578     345     250     216      32      32      29       3
                   3
 
HincII GTy'rAC

Cuts at:      0     435    1488 
   Size:        435    1053
 
MslI CAynn'nnrTG

Cuts at:      0     184     517     745    1006    1117    1213    1488 
   Size:        184     333     228     261     111      96     275

  Fragments arranged by size: 

                 333     275     261     228     184     111      96
 
MspA1I CmG'CkG

Cuts at:      0     989    1054    1350    1488 
   Size:        989      65     296     138

  Fragments arranged by size: 

                 989     296     138      65
 
NcoI C'CATG_G

Cuts at:      0     197    1488 
   Size:        197    1291
 
NspI r_CATG'y

Cuts at:      0      63    1011    1488 
   Size:         63     948     477

  Fragments arranged by size: 

                 948     477      63
 
PciI A'CATG_T

Cuts at:      0      59    1007    1488 
   Size:         59     948     481

  Fragments arranged by size: 

                 948     481      59
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1251    1488 
   Size:       1251     237
 
PfoI T'CCnGG_A

Cuts at:      0     299     923    1488 
   Size:        299     624     565

  Fragments arranged by size: 

                 624     565     299
 
PpuMI rG'GwC_Cy

Cuts at:      0    1385    1488 
   Size:       1385     103
 
PspOMI G'GGCC_C

Cuts at:      0     598    1488 
   Size:        598     890
 
PstI C_TGCA'G

Cuts at:      0     207     529     701    1488 
   Size:        207     322     172     787

  Fragments arranged by size: 

                 787     322     207     172
 
PvuII CAG'CTG

Cuts at:      0    1350    1488 
   Size:       1350     138
 
RsrII CG'GwC_CG

Cuts at:      0     658    1488 
   Size:        658     830
 
SacII CC_GC'GG

Cuts at:      0     990    1488 
   Size:        990     498
 
SfcI C'TryA_G

Cuts at:      0     203     525     697    1488 
   Size:        203     322     172     791

  Fragments arranged by size: 

                 791     322     203     172
 
SmlI C'TyrA_G

Cuts at:      0     244    1488 
   Size:        244    1244
 
StyI C'CwwG_G

Cuts at:      0     101     197    1488 
   Size:        101      96    1291

  Fragments arranged by size: 

                1291     101      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1167    1258    1360    1398    1488 
   Size:       1167      91     102      38      90

  Fragments arranged by size: 

                1167     102      91      90      38
 
TatI w'GTAC_w

Cuts at:      0      56    1010    1488 
   Size:         56     954     478

  Fragments arranged by size: 

                 954     478      56
 
Tth111I GACn'n_nGTC

Cuts at:      0    1189    1488 
   Size:       1189     299
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1209    1354    1488 
   Size:       1209     145     134

  Fragments arranged by size: 

                1209     145     134
 
ZraI GAC'GTC

Cuts at:      0     678    1038    1488 
   Size:        678     360     450

  Fragments arranged by size: 

                 678     450     360
 

 Enzymes that do cut:

     AarI    AatII     AccI   AflIII     AgeI     AhdI     AleI    AlwNI
     ApaI     AvaI     BanI    BanII     BbsI    BbvCI Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII
    Hin4I   HincII     MslI   MspA1I     NcoI     NspI     PciI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII    RsrII    SacII     SfcI
     SmlI     StyI    TaqII     TatI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI     AfeI    AflII     AlfI     AloI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI    BsiEI  BsiHKAI    BsiWI    BssSI    BstBI  BstZ17I
   Bsu36I     ClaI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRI
    EcoRV     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI     XbaI     XhoI     XmaI     XmnI
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