Restriction map for pF1KB7651
 (Linear) (Six-base) MAPSORT of: KIBB7651.seq  Check: 3028  from: 1  to: 990



KIBB7651.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AfeI AGC'GCT

Cuts at:      0     381     655     990 
   Size:        381     274     335

  Fragments arranged by size: 

                 381     335     274
 
AleI CACnn'nnGTG

Cuts at:      0     970     990 
   Size:        970      20
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     841     990 
   Size:        841     149
 
AlwNI CAG_nnn'CTG

Cuts at:      0     447     870     924     990 
   Size:        447     423      54      66

  Fragments arranged by size: 

                 447     423      66      54
 
ApaLI G'TGCA_C

Cuts at:      0     892     990 
   Size:        892      98
 
AvaI C'yCGr_G

Cuts at:      0      30     990 
   Size:         30     960
 
BanI G'GyrC_C

Cuts at:      0     157     268     364     399     990 
   Size:        157     111      96      35     591

  Fragments arranged by size: 

                 591     157     111      96      35
 
BanII G_rGCy'C

Cuts at:      0      47     184     990 
   Size:         47     137     806

  Fragments arranged by size: 

                 806     137      47
 
BbeI G_GCGC'C

Cuts at:      0     161     272     990 
   Size:        161     111     718

  Fragments arranged by size: 

                 718     161     111
 
BglI GCCn_nnn'nGGC

Cuts at:      0     248     257     700     990 
   Size:        248       9     443     290

  Fragments arranged by size: 

                 443     290     248       9
 
BglII A'GATC_T

Cuts at:      0     635     990 
   Size:        635     355
 
Bme1580I G_kGCm'C

Cuts at:      0     375     473     896     990 
   Size:        375      98     423      94

  Fragments arranged by size: 

                 423     375      98      94
 
BmgBI CAC'GTC

Cuts at:      0     617     968     990 
   Size:        617     351      22

  Fragments arranged by size: 

                 617     351      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     224     248     667     990 
   Size:        103     121      24     419     323

  Fragments arranged by size: 

                 419     323     121     103      24
 
BsaHI Gr'CG_yC

Cuts at:      0     158     269     494     589     976     990 
   Size:        158     111     225      95     387      14

  Fragments arranged by size: 

                 387     225     158     111      95      14
 
BsaWI w'CCGG_w

Cuts at:      0     584     990 
   Size:        584     406
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     221     990 
   Size:        221     769
 
BseYI C'CCAG_C

Cuts at:      0     440     990 
   Size:        440     550
 
BsiEI CG_ry'CG

Cuts at:      0     191     686     990 
   Size:        191     495     304

  Fragments arranged by size: 

                 495     304     191
 
BsiHKAI G_wGCw'C

Cuts at:      0       8     896     990 
   Size:          8     888      94

  Fragments arranged by size: 

                 888      94       8
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     762     990 
   Size:        762     228
 
Bsp1286I G_dGCh'C

Cuts at:      0       8      47     184     375     473     896     990 
   Size:          8      39     137     191      98     423      94

  Fragments arranged by size: 

                 423     191     137      98      94      39       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     720     990 
   Size:        720     270
 
BsrBI CCG'CTC

Cuts at:      0     211     417     990 
   Size:        211     206     573

  Fragments arranged by size: 

                 573     211     206
 
BsrFI r'CCGG_y

Cuts at:      0     292     402     454     752     990 
   Size:        292     110      52     298     238

  Fragments arranged by size: 

                 298     292     238     110      52
 
BssHII G'CGCG_C

Cuts at:      0     690     990 
   Size:        690     300
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     924     990 
   Size:        924      66
 
BstEII G'GTnAC_C

Cuts at:      0     568     990 
   Size:        568     422
 
BstYI r'GATC_y

Cuts at:      0      68     635     990 
   Size:         68     567     355

  Fragments arranged by size: 

                 567     355      68
 
BtgI C'CryG_G

Cuts at:      0     185     428     931     990 
   Size:        185     243     503      59

  Fragments arranged by size: 

                 503     243     185      59
 
EaeI y'GGCC_r

Cuts at:      0     188     449     683     701     990 
   Size:        188     261     234      18     289

  Fragments arranged by size: 

                 289     261     234     188      18
 
EagI C'GGCC_G

Cuts at:      0     188     683     990 
   Size:        188     495     307

  Fragments arranged by size: 

                 495     307     188
 
EarI CTCTTCn'nnn_

Cuts at:      0     847     913     990 
   Size:        847      66      77

  Fragments arranged by size: 

                 847      77      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     771     990 
   Size:        771     219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     224     248     667     990 
   Size:        103     121      24     419     323

  Fragments arranged by size: 

                 419     323     121     103      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     703     990 
   Size:        703     287
 
EcoO109I rG'GnC_Cy

Cuts at:      0     103     215     232     283     990 
   Size:        103     112      17      51     707

  Fragments arranged by size: 

                 707     112     103      51      17
 
FspI TGC'GCA

Cuts at:      0     728     990 
   Size:        728     262
 
HaeII r_GCGC'y

Cuts at:      0     161     272     383     657     712     990 
   Size:        161     111     111     274      55     278

  Fragments arranged by size: 

                 278     274     161     111     111      55
 
HincII GTy'rAC

Cuts at:      0     498     990 
   Size:        498     492
 
KasI G'GCGC_C

Cuts at:      0     157     268     990 
   Size:        157     111     722

  Fragments arranged by size: 

                 722     157     111
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     990 
   Size:        132     858
 
MscI TGG'CCA

Cuts at:      0     451     703     990 
   Size:        451     252     287

  Fragments arranged by size: 

                 451     287     252
 
MslI CAynn'nnrTG

Cuts at:      0     970     990 
   Size:        970      20
 
MspA1I CmG'CkG

Cuts at:      0     187     359     870     877     990 
   Size:        187     172     511       7     113

  Fragments arranged by size: 

                 511     187     172     113       7
 
NaeI GCC'GGC

Cuts at:      0     404     990 
   Size:        404     586
 
NarI GG'CG_CC

Cuts at:      0     158     269     990 
   Size:        158     111     721

  Fragments arranged by size: 

                 721     158     111
 
NgoMIV G'CCGG_C

Cuts at:      0     402     990 
   Size:        402     588
 
NotI GC'GGCC_GC

Cuts at:      0     188     990 
   Size:        188     802
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     447     990 
   Size:        447     543
 
PfoI T'CCnGG_A

Cuts at:      0     119     990 
   Size:        119     871
 
PpuMI rG'GwC_Cy

Cuts at:      0     103     232     283     990 
   Size:        103     129      51     707

  Fragments arranged by size: 

                 707     129     103      51
 
PstI C_TGCA'G

Cuts at:      0     277     635     830     920     929     990 
   Size:        277     358     195      90       9      61

  Fragments arranged by size: 

                 358     277     195      90      61       9
 
SacII CC_GC'GG

Cuts at:      0     188     990 
   Size:        188     802
 
SanDI GG'GwC_CC

Cuts at:      0     103     990 
   Size:        103     887
 
SapI GCTCTTCn'nnn_

Cuts at:      0     847     913     990 
   Size:        847      66      77

  Fragments arranged by size: 

                 847      77      66
 
SexAI A'CCwGG_T

Cuts at:      0     739     990 
   Size:        739     251
 
SfcI C'TryA_G

Cuts at:      0     273     631     826     916     925     990 
   Size:        273     358     195      90       9      65

  Fragments arranged by size: 

                 358     273     195      90      65       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     248     990 
   Size:        248     742
 
SfoI GGC'GCC

Cuts at:      0     159     270     990 
   Size:        159     111     720

  Fragments arranged by size: 

                 720     159     111
 
SmaI CCC'GGG

Cuts at:      0      32     990 
   Size:         32     958
 
StuI AGG'CCT

Cuts at:      0     844     990 
   Size:        844     146
 
StyI C'CwwG_G

Cuts at:      0     138     317     704     990 
   Size:        138     179     387     286

  Fragments arranged by size: 

                 387     286     179     138
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     736     990 
   Size:        736     254
 
XmaI C'CCGG_G

Cuts at:      0      30     990 
   Size:         30     960
 
XmnI GAAnn'nnTTC

Cuts at:      0     655     990 
   Size:        655     335
 

 Enzymes that do cut:

     AfeI     AleI     AlfI    AlwNI    ApaLI     AvaI     BanI    BanII
     BbeI     BglI    BglII Bme1580I    BmgBI     BpmI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrFI   BssHII   BstAPI   BstEII    BstYI     BtgI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NotI    PflMI     PfoI    PpuMI     PstI    SacII    SanDI     SapI
    SexAI     SfcI     SfiI     SfoI     SmaI     StuI     StyI    TaqII
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI     BsgI    BsiWI     BsmI    BspEI    BspHI
    BsrDI    BsrGI    BssSI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoRI
    EcoRV     FalI     FseI    FspAI    Hin4I  HindIII     HpaI     KpnI
     MfeI     MluI     NcoI     NdeI     NheI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
     SbfI     ScaI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com