Restriction map for pF1KE0464
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AfaI        AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr13I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRV       EcoT14I     EcoT22I     
FauI        FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvnI        MwoI        
NarI        NciI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          503  GTCTAC               GT^[AC][GT]AC
AciI           17  CCGC                 CCGC(-3/-1)
AclI          530  AACGTT               AA^CGTT
AflII         451  CTTAAG               C^TTAAG
Asp700I       298  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         221  GTGCTC               G[AT]GC[AT]^C
BciVI         489  GGATAC               GTATCC(6/5)
BclI          584  TGATCA               T^GATCA
BfaI          484  CTAG                 C^TAG
BfrI          451  CTTAAG               C^TTAAG
BmyI          221  GTGCTC               G[AGT]GC[ACT]^C
BpmI          245  CTGGAG               CTGGAG(16/14)
BsaAI          76  CACGTA               [CT]AC^GT[GA]
BsiHKAI       221  GTGCTC               G[AT]GC[AT]^C
BsmFI         626  GGGAC                GGGAC(10/14)
BsmI          141  GAATGC               GAATGC(1/-1)
Bsp1286I      221  GTGCTC               G[AGT]GC[ACT]^C
BsrDI         121  GCAATG               GCAATG(2/0)
BsrFI         148  GCCGGC               [GA]^CCGG[CT]
BssKI         244  CCTGG                ^CC[ACGT]GG
BstBI          51  TTCGAA               TT^CGAA
BstNI         244  CCTGG                CC^[AT]GG
BstXI         360  CCATCTTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        148  GCCGGC               [GA]^CCGG[CT]
EcoNI         163  CCTAAAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         391  GAATTC               G^AATTC
EcoRII        244  CCTGG                ^CC[AT]GG
FbaI          584  TGATCA               T^GATCA
HapII         149  CCGG                 C^CGG
HpaII         149  CCGG                 C^CGG
MaeI          484  CTAG                 C^TAG
MspI          149  CCGG                 C^CGG
MvaI          244  CCTGG                CC^[AT]GG
NaeI          148  GCCGGC               GCC^GGC
NgoMIV        148  GCCGGC               G^CCGGC
NlaIV         159  GGAGCC               GG[ACGT]^[ACGT]CC
NspV           51  TTCGAA               TT^CGAA
PleI          248  GAGTC                GAGTC(4/5)
PshAI         628  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      530  AACGTT               AA^CGTT
ScrFI         244  CCTGG                CC^[ACGT]GG
SfuI           51  TTCGAA               TT^CGAA
SmlI          451  CTTAAG               C^T[CT][GA]AG
SspI          229  AATATT               AAT^ATT
TfiI          268  GATTC                G^A[AT]TC
TspRI         426  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmnI          298  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcsI           48  AAATTC               [GA]^AATT[CT]
              391  GAATTC              
AluI          421  AGCT                 AG^CT
              482  AGCT                
ApoI           48  AAATTC               [GA]^AATT[CT]
              391  GAATTC              
BbsI          201  GAAGAC               GAAGAC(2/6)
              552  GAAGAC              
BpuAI         201  GAAGAC               GAAGAC(2/6)
              552  GAAGAC              
BsiYI         149  CCGGCATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              163  CCTAAAAAAGG         
BslI          149  CCGGCATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              163  CCTAAAAAAGG         
BsrI          609  ACTGG                ACTGG(1/-1)
              402  CCAGT               
BstF5I         25  GGATG                GGATG(2/0)
              573  GGATG               
FokI           25  GGATG                GGATG(9/13)
              573  GGATG               
HinfI         248  GAGTC                G^A[ACGT]TC
              268  GATTC               
MaeII          77  ACGT                 A^CGT
              531  ACGT                
NlaIII        380  CATG                 CATG^
              469  CATG                
SfcI          283  CTACAG               C^T[GA][CT]AG
              423  CTACAG              
TaqI           52  TCGA                 T^CGA
              187  TCGA                
TthHB8I        52  TCGA                 T^CGA
              187  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

BbvI        Cac8I       DpnII       Fnu4HI      HphI        
ItaI        MboI        MboII       MnlI        MseI        
NdeII       Sau3AI      Tru9I       TseI        Tsp509I     
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