Restriction map for pF1KE0382
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AclI        AflII       AflIII      AgeI        AhdI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BbeI        BbrPI       BcgI        BfaI        
BfrI        BglI        BlnI        BsaBI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Cfr10I      CpoI        DraI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRI       EcoT22I     
FseI        FspI        HindIII     HpaI        KasI        
MaeI        MamI        MfeI        MluI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         445  GACGTC               GACGT^C
Acc65I       1621  GGTACC               G^GTACC
AccIII       1037  TCCGGA               T^CCGGA
AcsI         2006  AAATTC               [GA]^AATT[CT]
AocI         1357  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       268  AGCGCT               AGC^GCT
ApoI         2006  AAATTC               [GA]^AATT[CT]
Asp700I      1629  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1621  GGTACC               G^GTACC
AspI          442  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1351  GGATCC               G^GATCC
BbvCI        1852  GCTGAGG              CCTCAGC(-5/-2)
BclI          686  TGATCA               T^GATCA
BlpI          132  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      132  GCTGAGC              GC^T[ACGT]AGC
BsaAI         948  TACGTA               [CT]AC^GT[GA]
BsaI          187  GAGACC               GGTCTC(1/5)
BseAI        1037  TCCGGA               T^CCGGA
BspDI         943  ATCGAT               AT^CGAT
BspEI        1037  TCCGGA               T^CCGGA
BsrBI        1690  CCGCTC               CCGCTC(-3/-3)
BstXI        1571  CCATCGGACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1357  CCTGAGG              CC^T[ACGT]AGG
CelII         132  GCTGAGC              GC^T[ACGT]AGC
ClaI          943  ATCGAT               AT^CGAT
DsaI          108  CCGCGG               C^C[GA][CT]GG
Eco47III      268  AGCGCT               AGC^GCT
Eco81I       1357  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1634  CCTTCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1804  GATATC               GAT^ATC
FbaI          686  TGATCA               T^GATCA
HgaI           86  GCGTC                GACGC(5/10)
KpnI         1621  GGTACC               GGTAC^C
KspI          108  CCGCGG               CCGC^GG
MroI         1037  TCCGGA               T^CCGGA
PflFI         442  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI           45  GAGCTC               GAGCT^C
SacII         108  CCGCGG               CCGC^GG
SalI           34  GTCGAC               G^TCGAC
SmlI           25  CTTGAG               C^T[CT][GA]AG
SnaBI         948  TACGTA               TAC^GTA
TfiI          524  GATTC                G^A[AT]TC
Tth111I       442  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1629  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI           34  GTCGAC               GT^[AC][GT]AC
              448  GTCTAC              
AcyI           85  GGCGTC               G[GA]^CG[CT]C
              445  GACGTC              
Alw44I        858  GTGCAC               G^TGCAC
             1595  GTGCAC              
ApaI         1401  GGGCCC               GGGCC^C
             1567  GGGCCC              
ApaLI         858  GTGCAC               G^TGCAC
             1595  GTGCAC              
BanI         1542  GGTGCC               G^G[CT][GA]CC
             1621  GGTACC              
BbsI          841  GTCTTC               GAAGAC(2/6)
             1372  GTCTTC              
BglII         362  AGATCT               A^GATCT
             1044  AGATCT              
BpuAI         841  GTCTTC               GAAGAC(2/6)
             1372  GTCTTC              
BsaHI          85  GGCGTC               G[GA]^CG[CT]C
              445  GACGTC              
BsaWI         190  ACCGGA               [AT]^CCGG[AT]
             1037  TCCGGA              
BseRI        1693  CTCCTC               GAGGAG(10/8)
             1360  GAGGAG              
BsgI         1920  CTGCAC               GTGCAG(16/14)
              666  GTGCAG              
BspHI         516  TCATGA               T^CATGA
             1376  TCATGA              
BstAPI       1518  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1644  GCACACAGTGC         
DrdI         1776  GACAGGGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2101  GACATCTTCGTC        
EarI          562  GAAGAG               CTCTTC(1/4)
             1771  GAAGAG              
Hin1I          85  GGCGTC               G[GA]^CG[CT]C
              445  GACGTC              
HincII         34  GTCGAC               GT[CT]^[GA]AC
              978  GTCAAC              
HindII         34  GTCGAC               GT[CT]^[GA]AC
              978  GTCAAC              
Ksp632I       562  GAAGAG               CTCTTC(1/4)
             1771  GAAGAG              
RcaI          516  TCATGA               T^CATGA
             1376  TCATGA              
SmaI          242  CCCGGG               CCC^GGG
              253  CCCGGG              
TaqI           35  TCGA                 T^CGA
              944  TCGA                
TthHB8I        35  TCGA                 T^CGA
              944  TCGA                
XcmI         1571  CCATCGGACTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1892  CCAACACCTTAATGG     
XmaCI         242  CCCGGG               C^CCGGG
              253  CCCGGG              
XmaI          242  CCCGGG               C^CCGGG
              253  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanII       BbvI        BciVI       BcnI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
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