Restriction map for pF1KE0382
 (Linear) (Six-base) MAPSORT of: KIEE0382.seq  Check: 6917  from: 1  to: 2130



KIEE0382.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AatII G_ACGT'C

Cuts at:      0     449    2130 
   Size:        449    1681
 
AccI GT'mk_AC

Cuts at:      0      35     449    2130 
   Size:         35     414    1681

  Fragments arranged by size: 

                1681     414      35
 
Acc65I G'GTAC_C

Cuts at:      0    1621    2130 
   Size:       1621     509
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123    2130 
   Size:        123    2007
 
AfeI AGC'GCT

Cuts at:      0     270    2130 
   Size:        270    1860
 
AleI CACnn'nnGTG

Cuts at:      0     178    1927    2130 
   Size:        178    1749     203

  Fragments arranged by size: 

                1749     203     178
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1945    2130 
   Size:       1945     185
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1077    1515    1524    1605    2130 
   Size:       1077     438       9      81     525

  Fragments arranged by size: 

                1077     525     438      81       9
 
ApaI G_GGCC'C

Cuts at:      0    1405    1571    2130 
   Size:       1405     166     559

  Fragments arranged by size: 

                1405     559     166
 
ApaLI G'TGCA_C

Cuts at:      0     858    1595    2130 
   Size:        858     737     535

  Fragments arranged by size: 

                 858     737     535
 
ApoI r'AATT_y

Cuts at:      0    2006    2130 
   Size:       2006     124
 
AvaI C'yCGr_G

Cuts at:      0     221     242     253    1498    1562    2130 
   Size:        221      21      11    1245      64     568

  Fragments arranged by size: 

                1245     568     221      64      21      11
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1486    1519    2130 
   Size:       1486      33     611

  Fragments arranged by size: 

                1486     611      33
 
BamHI G'GATC_C

Cuts at:      0    1351    2130 
   Size:       1351     779
 
BanI G'GyrC_C

Cuts at:      0    1542    1621    2130 
   Size:       1542      79     509

  Fragments arranged by size: 

                1542     509      79
 
BanII G_rGCy'C

Cuts at:      0      49    1405    1571    2127    2130 
   Size:         49    1356     166     556       3

  Fragments arranged by size: 

                1356     556     166      49       3
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     834    1365    2130 
   Size:        834     531     765

  Fragments arranged by size: 

                 834     765     531
 
BbvCI CC'TCA_GC

Cuts at:      0    1853    2130 
   Size:       1853     277
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1051    1487    1543    2130 
   Size:       1051     436      56     587

  Fragments arranged by size: 

                1051     587     436      56
 
BclI T'GATC_A

Cuts at:      0     686    2130 
   Size:        686    1444
 
BglII A'GATC_T

Cuts at:      0     362    1044    2130 
   Size:        362     682    1086

  Fragments arranged by size: 

                1086     682     362
 
BlpI GC'TnA_GC

Cuts at:      0     133    2130 
   Size:        133    1997
 
Bme1580I G_kGCm'C

Cuts at:      0     862    1405    1571    1599    2130 
   Size:        862     543     166      28     531

  Fragments arranged by size: 

                 862     543     531     166      28
 
BmgBI CAC'GTC

Cuts at:      0     654    1014    2130 
   Size:        654     360    1116

  Fragments arranged by size: 

                1116     654     360
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     735    2130 
   Size:        735    1395
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     756    2130 
   Size:        756    1374
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     285     781    1459    1659    1834    2035    2130 
   Size:         33     252     496     678     200     175     201      95

  Fragments arranged by size: 

                 678     496     252     201     200     175      95      33
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     876    1853    2130 
   Size:        876     977     277

  Fragments arranged by size: 

                 977     876     277
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46    2130 
   Size:         46    2084
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     181    2130 
   Size:        181    1949
 
BsaAI yAC'GTr

Cuts at:      0     950    2130 
   Size:        950    1180
 
BsaHI Gr'CG_yC

Cuts at:      0      86     446    2130 
   Size:         86     360    1684

  Fragments arranged by size: 

                1684     360      86
 
BsaWI w'CCGG_w

Cuts at:      0     190    1037    2130 
   Size:        190     847    1093

  Fragments arranged by size: 

                1093     847     190
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1669    1699    1985    2015    2130 
   Size:       1669      30     286      30     115

  Fragments arranged by size: 

                1669     286     115      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1375    1684    2130 
   Size:       1375     309     446

  Fragments arranged by size: 

                1375     446     309
 
BseYI C'CCAG_C

Cuts at:      0     213     399     641     836    1091    1398    2119    2130 
   Size:        213     186     242     195     255     307     721      11

  Fragments arranged by size: 

                 721     307     255     242     213     195     186      11
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     687    1905    2130 
   Size:        687    1218     225

  Fragments arranged by size: 

                1218     687     225
 
BsiHKAI G_wGCw'C

Cuts at:      0      49      68     862    1599    2130 
   Size:         49      19     794     737     531

  Fragments arranged by size: 

                 794     737     531      49      19
 
Bsp1286I G_dGCh'C

Cuts at:      0      49      68     862    1405    1571    1599    2127    2130 
   Size:         49      19     794     543     166      28     528       3

  Fragments arranged by size: 

                 794     543     528     166      49      28      19       3
            
 
BspEI T'CCGG_A

Cuts at:      0    1037    2130 
   Size:       1037    1093
 
BspHI T'CATG_A

Cuts at:      0     516    1376    2130 
   Size:        516     860     754

  Fragments arranged by size: 

                 860     754     516
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      47     461    1062    1523    2130 
   Size:         47     414     601     461     607

  Fragments arranged by size: 

                 607     601     461     414      47
 
BsrBI CCG'CTC

Cuts at:      0    1692    2130 
   Size:       1692     438
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1524    1650    2130 
   Size:       1524     126     480

  Fragments arranged by size: 

                1524     480     126
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1578    2130 
   Size:       1578     552
 
BstYI r'GATC_y

Cuts at:      0     362     590    1044    1351    1431    2130 
   Size:        362     228     454     307      80     699

  Fragments arranged by size: 

                 699     454     362     307     228      80
 
Bsu36I CC'TnA_GG

Cuts at:      0    1358    2130 
   Size:       1358     772
 
BtgI C'CryG_G

Cuts at:      0     108    2130 
   Size:        108    2022
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1743    2130 
   Size:       1743     387
 
BtsI GCAGTG_nn'

Cuts at:      0     780     799    1110    2130 
   Size:        780      19     311    1020

  Fragments arranged by size: 

                1020     780     311      19
 
ClaI AT'CG_AT

Cuts at:      0     944    2130 
   Size:        944    1186
 
DraIII CAC_nnn'GTG

Cuts at:      0     387     924    1650    2130 
   Size:        387     537     726     480

  Fragments arranged by size: 

                 726     537     480     387
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1782    2107    2130 
   Size:       1782     325      23

  Fragments arranged by size: 

                1782     325      23
 
EaeI y'GGCC_r

Cuts at:      0     111     339     626     779    1233    1487    1687    1988 
   Size:        111     228     287     153     454     254     200     301

Cuts at:   1988    2130 
   Size:        142

  Fragments arranged by size: 

                 454     301     287     254     228     200     153     142
                 111
 
EarI CTCTTCn'nnn_

Cuts at:      0     557    1766    2130 
   Size:        557    1209     364

  Fragments arranged by size: 

                1209     557     364
 
EcoICRI GAG'CTC

Cuts at:      0      47    2130 
   Size:         47    2083
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     123     285     781    1459    1659    1834    2035 
   Size:         33      90     162     496     678     200     175     201

Cuts at:   2035    2130 
   Size:         95

  Fragments arranged by size: 

                 678     496     201     200     175     162      95      90
                  33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1638    2130 
   Size:       1638     492
 
EcoO109I rG'GnC_Cy

Cuts at:      0     218    1402    1512    1567    1602    1971    2130 
   Size:        218    1184     110      55      35     369     159

  Fragments arranged by size: 

                1184     369     218     159     110      55      35
 
EcoRV GAT'ATC

Cuts at:      0    1806    2130 
   Size:       1806     324
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     328    1433    2121    2130 
   Size:        328    1105     688       9

  Fragments arranged by size: 

                1105     688     328       9
 
HaeII r_GCGC'y

Cuts at:      0     272    1317    1870    2130 
   Size:        272    1045     553     260

  Fragments arranged by size: 

                1045     553     272     260
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     280     312     594     626     961     993    1865    1897 
   Size:        280      32     282      32     335      32     872      32

Cuts at:   1897    1985    2017    2130 
   Size:         88      32     113

  Fragments arranged by size: 

                 872     335     282     280     113      88      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0      36     980    2130 
   Size:         36     944    1150

  Fragments arranged by size: 

                1150     944      36
 
KpnI G_GTAC'C

Cuts at:      0    1625    2130 
   Size:       1625     505
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     910    2130 
   Size:        317     593    1220

  Fragments arranged by size: 

                1220     593     317
 
MscI TGG'CCA

Cuts at:      0     341     781    1235    1489    2130 
   Size:        341     440     454     254     641

  Fragments arranged by size: 

                 641     454     440     341     254
 
MslI CAynn'nnrTG

Cuts at:      0     178     865    1294    1616    1747    1900    1927    2130 
   Size:        178     687     429     322     131     153      27     203

  Fragments arranged by size: 

                 687     429     322     203     178     153     131      27
            
 
MspA1I CmG'CkG

Cuts at:      0     110     399     645    1473    1521    1919    2130 
   Size:        110     289     246     828      48     398     211

  Fragments arranged by size: 

                 828     398     289     246     211     110      48
 
PfoI T'CCnGG_A

Cuts at:      0      49    1427    1436    2130 
   Size:         49    1378       9     694

  Fragments arranged by size: 

                1378     694      49       9
 
PpuMI rG'GwC_Cy

Cuts at:      0     218    1512    1971    2130 
   Size:        218    1294     459     159

  Fragments arranged by size: 

                1294     459     218     159
 
PspOMI G'GGCC_C

Cuts at:      0    1401    1567    2130 
   Size:       1401     166     563

  Fragments arranged by size: 

                1401     563     166
 
PstI C_TGCA'G

Cuts at:      0      44     476     719    1478    1520    2130 
   Size:         44     432     243     759      42     610

  Fragments arranged by size: 

                 759     610     432     243      44      42
 
PvuII CAG'CTG

Cuts at:      0    1473    1521    1919    2130 
   Size:       1473      48     398     211

  Fragments arranged by size: 

                1473     398     211      48
 
SacI G_AGCT'C

Cuts at:      0      49    2130 
   Size:         49    2081
 
SacII CC_GC'GG

Cuts at:      0     111    2130 
   Size:        111    2019
 
SalI G'TCGA_C

Cuts at:      0      34    2130 
   Size:         34    2096
 
SanDI GG'GwC_CC

Cuts at:      0     218    2130 
   Size:        218    1912
 
SfcI C'TryA_G

Cuts at:      0      40     472     715     787    1474    1516    1704    2130 
   Size:         40     432     243      72     687      42     188     426

  Fragments arranged by size: 

                 687     432     426     243     188      72      42      40
            
 
SmaI CCC'GGG

Cuts at:      0     244     255    2130 
   Size:        244      11    1875

  Fragments arranged by size: 

                1875     244      11
 
SmlI C'TyrA_G

Cuts at:      0      25    2130 
   Size:         25    2105
 
SnaBI TAC'GTA

Cuts at:      0     950    2130 
   Size:        950    1180
 
StyI C'CwwG_G

Cuts at:      0     375     427    1224    1974    2130 
   Size:        375      52     797     750     156

  Fragments arranged by size: 

                 797     750     375     156      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     974    1570    2130 
   Size:        974     596     560

  Fragments arranged by size: 

                 974     596     560
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     759     791    1568    1600    1669    1701    2130 
   Size:        759      32     777      32      69      32     429

  Fragments arranged by size: 

                 777     759     429      69      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     445    2130 
   Size:        445    1685
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1578    1899    2130 
   Size:       1578     321     231

  Fragments arranged by size: 

                1578     321     231
 
XmaI C'CCGG_G

Cuts at:      0     242     253    2130 
   Size:        242      11    1877

  Fragments arranged by size: 

                1877     242      11
 
XmnI GAAnn'nnTTC

Cuts at:      0    1633    2130 
   Size:       1633     497
 
ZraI GAC'GTC

Cuts at:      0     447    2130 
   Size:        447    1683
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII
     KpnI     MmeI     MscI     MslI   MspA1I     PfoI    PpuMI   PspOMI
     PstI    PvuII     SacI    SacII     SalI    SanDI     SfcI     SmaI
     SmlI    SnaBI     StyI    TaqII     TstI  Tth111I     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII   AflIII     AgeI     AhdI     AloI     AscI
     AseI    AsiSI    AvrII     BbeI     BcgI    BfrBI     BglI     BmtI
    BsaBI    BsiEI    BsiWI     BsmI    BsmBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    CspCI     DraI     EagI
     EciI    EcoRI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     XbaI     XhoI
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