Restriction map for pF1KB7214
 (Linear) (Six-base) MAPSORT of: KIBB7214.seq  Check: 633  from: 1  to: 879



KIBB7214.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     270     879 
   Size:        270     609
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     182     640     879 
   Size:         34     148     458     239

  Fragments arranged by size: 

                 458     239     148      34
 
AleI CACnn'nnGTG

Cuts at:      0     213     340     427     879 
   Size:        213     127      87     452

  Fragments arranged by size: 

                 452     213     127      87
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     375     879 
   Size:        375     504
 
AlwNI CAG_nnn'CTG

Cuts at:      0     160     599     879 
   Size:        160     439     280

  Fragments arranged by size: 

                 439     280     160
 
ApaI G_GGCC'C

Cuts at:      0     172     879 
   Size:        172     707
 
ApoI r'AATT_y

Cuts at:      0     528     570     650     879 
   Size:        528      42      80     229

  Fragments arranged by size: 

                 528     229      80      42
 
AvaI C'yCGr_G

Cuts at:      0      20      61     879 
   Size:         20      41     818

  Fragments arranged by size: 

                 818      41      20
 
BanI G'GyrC_C

Cuts at:      0      87     319     879 
   Size:         87     232     560

  Fragments arranged by size: 

                 560     232      87
 
BanII G_rGCy'C

Cuts at:      0     148     172     879 
   Size:        148      24     707

  Fragments arranged by size: 

                 707     148      24
 
BbeI G_GCGC'C

Cuts at:      0      91     879 
   Size:         91     788
 
Bme1580I G_kGCm'C

Cuts at:      0     172     879 
   Size:        172     707
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      81     879 
   Size:         81     798
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     167     879 
   Size:        167     712
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     493     879 
   Size:        137     356     386

  Fragments arranged by size: 

                 386     356     137
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     441     879 
   Size:        441     438
 
BsaAI yAC'GTr

Cuts at:      0     338     879 
   Size:        338     541
 
BsaHI Gr'CG_yC

Cuts at:      0      88     879 
   Size:         88     791
 
BsaWI w'CCGG_w

Cuts at:      0     607     879 
   Size:        607     272
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     133     260     879 
   Size:        100      33     127     619

  Fragments arranged by size: 

                 619     127     100      33
 
BseYI C'CCAG_C

Cuts at:      0     599     857     879 
   Size:        599     258      22

  Fragments arranged by size: 

                 599     258      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     172     879 
   Size:        148      24     707

  Fragments arranged by size: 

                 707     148      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     270     879 
   Size:        270     609
 
BstBI TT'CG_AA

Cuts at:      0     489     879 
   Size:        489     390
 
BstEII G'GTnAC_C

Cuts at:      0     327     603     879 
   Size:        327     276     276

  Fragments arranged by size: 

                 327     276     276
 
Bsu36I CC'TnA_GG

Cuts at:      0     563     879 
   Size:        563     316
 
BtsI GCAGTG_nn'

Cuts at:      0     208     357     879 
   Size:        208     149     522

  Fragments arranged by size: 

                 522     208     149
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     413     448     879 
   Size:        413      35     431

  Fragments arranged by size: 

                 431     413      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     835     879 
   Size:        835      44
 
EaeI y'GGCC_r

Cuts at:      0     219     879 
   Size:        219     660
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     137     182     493     640     879 
   Size:         34     103      45     311     147     239

  Fragments arranged by size: 

                 311     239     147     103      45      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115     879 
   Size:        115     764
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     157     169     879 
   Size:         24     133      12     710

  Fragments arranged by size: 

                 710     133      24      12
 
HaeII r_GCGC'y

Cuts at:      0      91     879 
   Size:         91     788
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     384     416     440     472     879 
   Size:        384      32      24      32     407

  Fragments arranged by size: 

                 407     384      32      32      24
 
KasI G'GCGC_C

Cuts at:      0      87     879 
   Size:         87     792
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     540     879 
   Size:        540     339
 
MslI CAynn'nnrTG

Cuts at:      0     213     340     383     427     879 
   Size:        213     127      43      44     452

  Fragments arranged by size: 

                 452     213     127      44      43
 
NarI GG'CG_CC

Cuts at:      0      88     879 
   Size:         88     791
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     599     879 
   Size:        599     280
 
PfoI T'CCnGG_A

Cuts at:      0     153     879 
   Size:        153     726
 
PmlI CAC'GTG

Cuts at:      0     338     879 
   Size:        338     541
 
PpuMI rG'GwC_Cy

Cuts at:      0     157     879 
   Size:        157     722
 
PspOMI G'GGCC_C

Cuts at:      0     168     879 
   Size:        168     711
 
SexAI A'CCwGG_T

Cuts at:      0     592     879 
   Size:        592     287
 
SfoI GGC'GCC

Cuts at:      0      89     879 
   Size:         89     790
 
SmaI CCC'GGG

Cuts at:      0      22      63     879 
   Size:         22      41     816

  Fragments arranged by size: 

                 816      41      22
 
SrfI GCCC'GGGC

Cuts at:      0      63     879 
   Size:         63     816
 
StuI AGG'CCT

Cuts at:      0     715     879 
   Size:        715     164
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     330     591     879 
   Size:        330     261     288

  Fragments arranged by size: 

                 330     288     261
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     253     285     349     381     741     773     879 
   Size:        253      32      64      32     360      32     106

  Fragments arranged by size: 

                 360     253     106      64      32      32      32
 
XmaI C'CCGG_G

Cuts at:      0      20      61     879 
   Size:         20      41     818

  Fragments arranged by size: 

                 818      41      20
 
XmnI GAAnn'nnTTC

Cuts at:      0     653     780     879 
   Size:        653     127      99

  Fragments arranged by size: 

                 653     127      99
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbeI Bme1580I     BmrI     BplI     BpmI     BsaI
    BsaAI    BsaHI    BsaWI    BseRI    BseYI Bsp1286I    BspMI    BstBI
   BstEII   Bsu36I     BtsI    CspCI     DrdI     EaeI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I     KasI     MmeI     MslI     NarI    PflMI
     PfoI     PmlI    PpuMI   PspOMI    SexAI     SfoI     SmaI     SrfI
     StuI    TaqII     TstI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI   Bpu10I    BpuEI    BsaBI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     ClaI     DraI   DraIII     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     StyI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     ZraI
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