Restriction map for pF1KE0015
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tth111I     
Van91I      XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AhdI           18  GACTGATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1588  GGGCCC               GGGCC^C
AspEI          18  GACTGATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1940  CCCGGG               C^[CT]CG[GA]G
BalI         1890  TGGCCA               TGG^CCA
BanI         1849  GGCACC               G^G[CT][GA]CC
BsaBI         937  GATAATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          489  GAATGC               GAATGC(1/-1)
BsoBI        1940  CCCGGG               C^[CT]CG[GA]G
BsrDI         341  CATTGC               GCAATG(2/0)
BstBI        1454  TTCGAA               TT^CGAA
BstEII        499  GGTAACC              G^GT[ACGT]ACC
BstF5I        955  CATCC                GGATG(2/0)
BstPI         499  GGTAACC              G^GT[ACGT]ACC
DraI         1207  TTTAAA               TTT^AAA
Eam1105I       18  GACTGATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         727  CCTCTGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       499  GGTAACC              G^GT[ACGT]ACC
FokI          955  CATCC                GGATG(9/13)
MamI          937  GATAATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1890  TGGCCA               TGG^CCA
MscI         1890  TGGCCA               TGG^CCA
MspA1I       1550  CAGCTG               C[AC]G^C[GT]G
NspV         1454  TTCGAA               TT^CGAA
PvuII        1550  CAGCTG               CAG^CTG
ScaI         1084  AGTACT               AGT^ACT
SfaNI         631  GCATC                GCATC(5/9)
SfuI         1454  TTCGAA               TT^CGAA
SmaI         1940  CCCGGG               CCC^GGG
SmlI         1286  CTCAAG               C^T[CT][GA]AG
Tsp45I       1546  GTCAC                ^GT[GC]AC
XbaI         1144  TCTAGA               T^CTAGA
XmaCI        1940  CCCGGG               C^CCGGG
XmaI         1940  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI          265  CCGC                 CCGC(-3/-1)
              997  CCGC                
AlwI         1904  GATCC                GGATC(4/5)
             1291  GGATC               
AlwNI        1094  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1538  CAGGACCTG           
BanII        1588  GGGCCC               G[GA]GC[CT]^C
             1943  GGGCTC              
BglII         763  AGATCT               A^GATCT
             1600  AGATCT              
BmyI         1588  GGGCCC               G[AGT]GC[ACT]^C
             1943  GGGCTC              
BpmI         1673  CTGGAG               CTGGAG(16/14)
             1867  CTGGAG              
BsmAI         951  GAGAC                GTCTC(1/5)
             1013  GAGAC               
BsmFI         894  GGGAC                GGGAC(10/14)
             1250  GTCCC               
Bsp1286I     1588  GGGCCC               G[AGT]GC[ACT]^C
             1943  GGGCTC              
BsrI          576  ACTGG                ACTGG(1/-1)
             1450  CCAGT               
EaeI         1890  TGGCCA               [CT]^GGCC[GA]
             1912  CGGCCA              
EarI          775  GAAGAG               CTCTTC(1/4)
             1792  GAAGAG              
HapII        1719  CCGG                 C^CGG
             1941  CCGG                
HincII        118  GTTGAC               GT[CT]^[GA]AC
              534  GTCAAC              
HindII        118  GTTGAC               GT[CT]^[GA]AC
              534  GTCAAC              
HindIII       216  AAGCTT               A^AGCTT
             1391  AAGCTT              
HpaII        1719  CCGG                 C^CGG
             1941  CCGG                
HphI          175  GGTGA                GGTGA(8/7)
             1885  GGTGA               
Ksp632I       775  GAAGAG               CTCTTC(1/4)
             1792  GAAGAG              
MaeII         683  ACGT                 A^CGT
             1317  ACGT                
MspI         1719  CCGG                 C^CGG
             1941  CCGG                
PleI          151  GACTC                GAGTC(4/5)
             1015  GACTC               
SfcI          567  CTATAG               C^T[GA][CT]AG
             1295  CTGTAG              
SspI          241  AATATT               AAT^ATT
             1572  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AvaII       
BbsI        BbvI        BcnI        BfaI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BssKI       
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      Fnu4HI      
HaeIII      HinfI       ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
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