Restriction map for pF1KE0015
 (Linear) (Six-base) MAPSORT of: KIEE0015.seq  Check: 3522  from: 1  to: 1953



KIEE0015.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     437     603     616     788    1076    1121    1133 
   Size:        272     165     166      13     172     288      45      12

Cuts at:   1133    1953 
   Size:        820

  Fragments arranged by size: 

                 820     288     272     172     166     165      45      13
                  12
 
AhdI GACnn_n'nnGTC

Cuts at:      0      23    1953 
   Size:         23    1930
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1099    1543    1953 
   Size:       1099     444     410

  Fragments arranged by size: 

                1099     444     410
 
ApaI G_GGCC'C

Cuts at:      0    1592    1953 
   Size:       1592     361
 
ApoI r'AATT_y

Cuts at:      0      49     385    1023    1768    1953 
   Size:         49     336     638     745     185

  Fragments arranged by size: 

                 745     638     336     185      49
 
AvaI C'yCGr_G

Cuts at:      0    1940    1953 
   Size:       1940      13
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1538    1571    1953 
   Size:       1538      33     382

  Fragments arranged by size: 

                1538     382      33
 
BanI G'GyrC_C

Cuts at:      0    1849    1953 
   Size:       1849     104
 
BanII G_rGCy'C

Cuts at:      0    1592    1947    1953 
   Size:       1592     355       6

  Fragments arranged by size: 

                1592     355       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1121    1518    1709    1953 
   Size:       1121     397     191     244

  Fragments arranged by size: 

                1121     397     244     191
 
BglII A'GATC_T

Cuts at:      0     763    1600    1953 
   Size:        763     837     353

  Fragments arranged by size: 

                 837     763     353
 
Bme1580I G_kGCm'C

Cuts at:      0    1592    1953 
   Size:       1592     361
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     659     974    1953 
   Size:        659     315     979

  Fragments arranged by size: 

                 979     659     315
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1694    1888    1953 
   Size:       1694     194      65

  Fragments arranged by size: 

                1694     194      65
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1271    1953 
   Size:       1271     682
 
BsaBI GATnn'nnATC

Cuts at:      0     941    1953 
   Size:        941    1012
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     467     716     950    1953 
   Size:        467     249     234    1003

  Fragments arranged by size: 

                1003     467     249     234
 
BsmI GAATG_Cn'

Cuts at:      0     495    1953 
   Size:        495    1458
 
Bsp1286I G_dGCh'C

Cuts at:      0    1592    1947    1953 
   Size:       1592     355       6

  Fragments arranged by size: 

                1592     355       6
 
BsrDI GCAATG_nn'

Cuts at:      0     340    1953 
   Size:        340    1613
 
BstBI TT'CG_AA

Cuts at:      0    1455    1953 
   Size:       1455     498
 
BstEII G'GTnAC_C

Cuts at:      0     499    1953 
   Size:        499    1454
 
BstYI r'GATC_y

Cuts at:      0     763    1291    1600    1953 
   Size:        763     528     309     353

  Fragments arranged by size: 

                 763     528     353     309
 
BtsI GCAGTG_nn'

Cuts at:      0    1053    1953 
   Size:       1053     900
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     150     185    1953 
   Size:        150      35    1768

  Fragments arranged by size: 

                1768     150      35
 
DraI TTT'AAA

Cuts at:      0    1209    1953 
   Size:       1209     744
 
EaeI y'GGCC_r

Cuts at:      0    1890    1912    1953 
   Size:       1890      22      41

  Fragments arranged by size: 

                1890      41      22
 
EarI CTCTTCn'nnn_

Cuts at:      0     770    1787    1953 
   Size:        770    1017     166

  Fragments arranged by size: 

                1017     770     166
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     437     603     616     788    1076    1121    1133 
   Size:        272     165     166      13     172     288      45      12

Cuts at:   1133    1694    1888    1953 
   Size:        561     194      65

  Fragments arranged by size: 

                 561     288     272     194     172     166     165      65
                  45      13      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     731    1953 
   Size:        731    1222
 
EcoO109I rG'GnC_Cy

Cuts at:      0     721     780    1249    1522    1540    1953 
   Size:        721      59     469     273      18     413

  Fragments arranged by size: 

                 721     469     413     273      59      18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1106    1953 
   Size:       1106     847
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       9      41     125     157    1354    1386    1953 
   Size:          9      32      84      32    1197      32     567

  Fragments arranged by size: 

                1197     567      84      32      32      32       9
 
HincII GTy'rAC

Cuts at:      0     120     536    1953 
   Size:        120     416    1417

  Fragments arranged by size: 

                1417     416     120
 
HindIII A'AGCT_T

Cuts at:      0     216    1391    1953 
   Size:        216    1175     562

  Fragments arranged by size: 

                1175     562     216
 
MscI TGG'CCA

Cuts at:      0    1892    1953 
   Size:       1892      61
 
MslI CAynn'nnrTG

Cuts at:      0     560    1196    1424    1953 
   Size:        560     636     228     529

  Fragments arranged by size: 

                 636     560     529     228
 
MspA1I CmG'CkG

Cuts at:      0    1552    1953 
   Size:       1552     401
 
PpuMI rG'GwC_Cy

Cuts at:      0     721     780    1249    1522    1540    1953 
   Size:        721      59     469     273      18     413

  Fragments arranged by size: 

                 721     469     413     273      59      18
 
PsiI TTA'TAA

Cuts at:      0    1304    1953 
   Size:       1304     649
 
PspOMI G'GGCC_C

Cuts at:      0    1588    1953 
   Size:       1588     365
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1149    1181    1953 
   Size:       1149      32     772

  Fragments arranged by size: 

                1149     772      32
 
PvuII CAG'CTG

Cuts at:      0    1552    1953 
   Size:       1552     401
 
ScaI AGT'ACT

Cuts at:      0    1086    1953 
   Size:       1086     867
 
SfcI C'TryA_G

Cuts at:      0     567    1295    1953 
   Size:        567     728     658

  Fragments arranged by size: 

                 728     658     567
 
SmaI CCC'GGG

Cuts at:      0    1942    1953 
   Size:       1942      11
 
SmlI C'TyrA_G

Cuts at:      0    1286    1953 
   Size:       1286     667
 
SspI AAT'ATT

Cuts at:      0     243    1574    1953 
   Size:        243    1331     379

  Fragments arranged by size: 

                1331     379     243
 
TatI w'GTAC_w

Cuts at:      0    1084    1953 
   Size:       1084     869
 
XbaI T'CTAG_A

Cuts at:      0    1144    1953 
   Size:       1144     809
 
XmaI C'CCGG_G

Cuts at:      0    1940    1953 
   Size:       1940      13
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApaI     ApoI     AvaI     BaeI     BanI
    BanII     BbsI    BglII Bme1580I     BplI     BpmI    BpuEI    BsaBI
    BseRI     BsmI Bsp1286I    BsrDI    BstBI   BstEII    BstYI     BtsI
    CspCI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FalI
    Hin4I   HincII  HindIII     MscI     MslI   MspA1I    PpuMI     PsiI
   PspOMI     PsrI    PvuII     ScaI     SfcI     SmaI     SmlI     SspI
     TatI     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI    PspXI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI     XmnI
     ZraI
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