Restriction map for
pF1KE0544
(Circular) (Six-base) MAPSORT of: pF1KE0544.seq Check: 3588 from: 1 to: 4394
pF1KE0544 4394 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1101 1101
Size: 4394
AccI GT'mk_AC
Cuts at: 836 1432 836
Size: 596 3798
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 745 1204 2219 2651 3398 3666 357
Size: 388 459 1015 432 747 268 1085
Fragments arranged by size:
1085 1015 747 459 432 388 268
AfeI AGC'GCT
Cuts at: 730 730
Size: 4394
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1349 1349
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 984 1066 1699 3266 984
Size: 82 633 1567 2112
Fragments arranged by size:
2112 1567 633 82
ApaI G_GGCC'C
Cuts at: 156 156
Size: 4394
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 1398 3747 4003 1398
Size: 2349 256 1789
Fragments arranged by size:
2349 1789 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 814 1414 814
Size: 600 3794
AvrII C'CTAG_G
Cuts at: 360 360
Size: 4394
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 113 1038 1410 1824 2072 2107 113
Size: 925 372 414 248 35 2400
Fragments arranged by size:
2400 925 414 372 248 35
BanII G_rGCy'C
Cuts at: 156 1408 2438 156
Size: 1252 1030 2112
Fragments arranged by size:
2112 1252 1030
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 320 320
Size: 4394
BbvCI CC'TCA_GC
Cuts at: 1372 1372
Size: 4394
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 540 1914 540
Size: 1374 3020
BglII A'GATC_T
Cuts at: 794 1909 3946 794
Size: 1115 2037 1242
Fragments arranged by size:
2037 1242 1115
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 156 2019 2112 3168 156
Size: 1863 93 1056 1382
Fragments arranged by size:
1863 1382 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 904 1606 1687 2022 4166 904
Size: 702 81 335 2144 1132
Fragments arranged by size:
2144 1132 702 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 273 532 268
Size: 5 259 4130
Fragments arranged by size:
4130 259 5
Bpu10I CC'TnA_GC
Cuts at: 1372 1372
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 895 1570 2449 2941 3239 319
Size: 576 675 879 492 298 1474
Fragments arranged by size:
1474 879 675 576 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 495 1063 1298 4098 495
Size: 568 235 2800 791
Fragments arranged by size:
2800 791 568 235
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1237 1267 1237
Size: 30 4364
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 805 805
Size: 4394
BseYI C'CCAG_C
Cuts at: 262 301 977 1385 1819 2577 3154 3650 262
Size: 39 676 408 434 758 577 496 1006
Fragments arranged by size:
1006 758 676 577 496 434 408 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 4394
BsiEI CG_ry'CG
Cuts at: 104 1274 1936 1982 3190 104
Size: 1170 662 46 1208 1308
Fragments arranged by size:
1308 1208 1170 662 46
BsiHKAI G_wGCw'C
Cuts at: 254 1408 2186 2376 3168 254
Size: 1154 778 190 792 1480
Fragments arranged by size:
1480 1154 792 778 190
BsmI GAATG_Cn'
Cuts at: 587 712 966 587
Size: 125 254 4015
Fragments arranged by size:
4015 254 125
Bsp1286I G_dGCh'C
Cuts at: 156 254 1408 2019 2112 2186 2376 2438 3168
Size: 98 1154 611 93 74 190 62 730
Cuts at: 3168 156
Size: 1382
Fragments arranged by size:
1382 1154 730 611 190 98 93 74
62
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 866 968 3570 866
Size: 102 2602 1690
Fragments arranged by size:
2602 1690 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 440 1101 1444 1658 1960 2341 332
Size: 108 661 343 214 302 381 2385
Fragments arranged by size:
2385 661 381 343 302 214 108
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 236 2392 2573 2782 236
Size: 2156 181 209 1848
Fragments arranged by size:
2156 1848 209 181
BssSI C'ACGA_G
Cuts at: 1043 2665 3023 1043
Size: 1622 358 2414
Fragments arranged by size:
2414 1622 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstEII G'GTnAC_C
Cuts at: 343 343
Size: 4394
BstYI r'GATC_y
Cuts at: 1 794 1419 1886 1909 2244 2490 3502 3588
Size: 793 625 467 23 335 246 1012 86
Cuts at: 3588 3600 3946 1
Size: 12 346 449
Fragments arranged by size:
1012 793 625 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 410 698 767 1169 410
Size: 288 69 402 3635
Fragments arranged by size:
3635 402 288 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 358 1637 358
Size: 1279 3115
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 487 2100 2958 487
Size: 1613 858 1923
Fragments arranged by size:
1923 1613 858
EaeI y'GGCC_r
Cuts at: 770 932 1157 1271 1979 2153 2544 2571 3916
Size: 162 225 114 708 174 391 27 1345
Cuts at: 3916 770
Size: 1248
Fragments arranged by size:
1345 1248 708 391 225 174 162 114
27
EagI C'GGCC_G
Cuts at: 1271 1979 1271
Size: 708 3686
EarI CTCTTCn'nnn_
Cuts at: 136 517 2417 2627 136
Size: 381 1900 210 1903
Fragments arranged by size:
1903 1900 381 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 273 357 532 745 1204 2219 2651 3398
Size: 5 84 175 213 459 1015 432 747
Cuts at: 3398 3666 268
Size: 268 996
Fragments arranged by size:
1015 996 747 459 432 268 213 175
84 5
EcoNI CCTnn'n_nnAGG
Cuts at: 1186 1186
Size: 4394
EcoO109I rG'GnC_Cy
Cuts at: 122 144 152 153 1240 1533 122
Size: 22 8 1 1087 293 2983
Fragments arranged by size:
2983 1087 293 22 8 1
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 732 1828 2076 3098 4077 732
Size: 1096 248 1022 979 1049
Fragments arranged by size:
1096 1049 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 746 778 779 811 1062 1094 1130 1162 2236
Size: 32 1 32 251 32 36 32 1074
Cuts at: 2236 2266 2268 2298 746
Size: 30 2 30 2842
Fragments arranged by size:
2842 1074 251 36 32 32 32 32
30 30 2 1
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
HindIII A'AGCT_T
Cuts at: 337 337
Size: 4394
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 730 1034 3065 3249 730
Size: 304 2031 184 1875
Fragments arranged by size:
2031 1875 304 184
MscI TGG'CCA
Cuts at: 772 934 2155 772
Size: 162 1221 3011
Fragments arranged by size:
3011 1221 162
MslI CAynn'nnrTG
Cuts at: 163 271 2383 2510 163
Size: 108 2112 127 2047
Fragments arranged by size:
2112 2047 127 108
MspA1I CmG'CkG
Cuts at: 420 981 1063 1505 1819 2179 3192 3437 420
Size: 561 82 442 314 360 1013 245 1377
Fragments arranged by size:
1377 1013 561 442 360 314 245 82
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 410 698 767 410
Size: 288 69 4037
Fragments arranged by size:
4037 288 69
NdeI CA'TA_TG
Cuts at: 1215 1215
Size: 4394
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 908 1447 1947 2854 908
Size: 539 500 907 2448
Fragments arranged by size:
2448 907 539 500
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 684 684
Size: 4394
PfoI T'CCnGG_A
Cuts at: 509 509
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 144 144
Size: 4394
PspOMI G'GGCC_C
Cuts at: 152 152
Size: 4394
PstI C_TGCA'G
Cuts at: 299 944 1112 1441 2126 299
Size: 645 168 329 685 2567
Fragments arranged by size:
2567 685 645 329 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 420 981 1063 1819 2179 420
Size: 561 82 756 360 2635
Fragments arranged by size:
2635 756 561 360 82
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 295 837 940 1108 1341 1437 2122 3115
Size: 262 542 103 168 233 96 685 993
Cuts at: 3115 3306 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 542 262 233 191 168
103 96
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 334 910 1549 2464 2956 3218 334
Size: 576 639 915 492 262 1510
Fragments arranged by size:
1510 915 639 576 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StuI AGG'CCT
Cuts at: 1228 1228
Size: 4394
StyI C'CwwG_G
Cuts at: 117 226 308 360 410 698 767 1349 1528
Size: 109 82 52 50 288 69 582 179
Cuts at: 1528 1882 117
Size: 354 2629
Fragments arranged by size:
2629 582 354 288 179 109 82 69
52 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1977 2110 2569 2778 1977
Size: 133 459 209 3593
Fragments arranged by size:
3593 459 209 133
TatI w'GTAC_w
Cuts at: 3909 3909
Size: 4394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 417 705 1152 1889 270
Size: 147 288 447 737 2775
Fragments arranged by size:
2775 737 447 288 147
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI BaeI BcgI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI