Restriction map for pF1KB6667
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BciVI       BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRV       EcoT14I     FauI        
FseI        FspI        HaeIII      HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NotI        NruI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflII         486  CTTAAG               C^TTAAG
Alw44I        614  GTGCAC               G^TGCAC
AlwI          429  GGATC                GGATC(4/5)
AlwNI         506  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          586  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       573  AGCGCT               AGC^GCT
ApaLI         614  GTGCAC               G^TGCAC
BcgI         1170  GCATATACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          273  TGATCA               T^GATCA
BfrI          486  CTTAAG               C^TTAAG
BsaAI         122  TACGTG               [CT]AC^GT[GA]
BsgI          651  GTGCAG               GTGCAG(16/14)
BstYI         429  GGATCT               [GA]^GATC[CT]
Bsu36I        586  CCTCAGG              CC^T[ACGT]AGG
CfoI          574  GCGC                 GCG^C
Eco47III      573  AGCGCT               AGC^GCT
Eco81I        586  CCTCAGG              CC^T[ACGT]AGG
FbaI          273  TGATCA               T^GATCA
HaeII         573  AGCGCT               [GA]GCGC^[CT]
HhaI          574  GCGC                 GCG^C
HinP1I        574  GCGC                 G^CGC
MflI          429  GGATCT               [GA]^GATC[CT]
MslI         1258  CACTTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        139  CAGCTG               C[AC]G^C[GT]G
NheI          462  GCTAGC               G^CTAGC
NlaIV        1035  GGACCC               GG[ACGT]^[ACGT]CC
PaeR7I       1178  CTCGAG               C^TCGAG
PstI           74  CTGCAG               CTGCA^G
PvuII         139  CAGCTG               CAG^CTG
SfcI           74  CTGCAG               C^T[GA][CT]AG
SmaI         1071  CCCGGG               CCC^GGG
XhoI         1178  CTCGAG               C^TCGAG
XhoII         429  GGATCT               [GA]^GATC[CT]
XmaCI        1071  CCCGGG               C^CCGGG
XmaI         1071  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          548  GTAC                 GT^AC
             1144  GTAC                
Asp700I       417  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              439  GAAGAATTTC          
AspHI         528  GTGCTC               G[AT]GC[AT]^C
              614  GTGCAC              
AvaI         1071  CCCGGG               C^[CT]CG[GA]G
             1178  CTCGAG              
AvaII        1035  GGACC                G^G[AT]CC
             1090  GGACC               
BbvI         1098  GCAGC                GCAGC(8/12)
              976  GCTGC               
BfaI          353  CTAG                 C^TAG
              463  CTAG                
BmyI          528  GTGCTC               G[AGT]GC[ACT]^C
              614  GTGCAC              
BpmI          746  CTCCAG               CTGGAG(16/14)
              992  CTGGAG              
BsaI          753  GAGACC               GGTCTC(1/5)
              995  GAGACC              
BsiHKAI       528  GTGCTC               G[AT]GC[AT]^C
              614  GTGCAC              
BsiYI         396  CCATGAAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1026  CCCACTTCTGG         
BslI          396  CCATGAAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1026  CCCACTTCTGG         
BsoBI        1071  CCCGGG               C^[CT]CG[GA]G
             1178  CTCGAG              
Bsp1286I      528  GTGCTC               G[AGT]GC[ACT]^C
              614  GTGCAC              
Cfr13I       1035  GGACC                G^G[ACGT]CC
             1090  GGACC               
DpnII         274  GATC                 ^GATC
              430  GATC                
EcoRI         195  GAATTC               G^AATTC
             1189  GAATTC              
Fnu4HI        976  GCTGC                GC^[ACGT]GC
             1098  GCAGC               
ItaI          976  GCTGC                GC^[ACGT]GC
             1098  GCAGC               
MaeI          353  CTAG                 C^TAG
              463  CTAG                
MaeII         123  ACGT                 A^CGT
              778  ACGT                
MboI          274  GATC                 ^GATC
              430  GATC                
MwoI           34  GCGTATGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              576  GCTGTTTAAGC         
NdeII         274  GATC                 ^GATC
              430  GATC                
NspI           55  GCATGC               [GA]CATG^[CT]
              458  GCATGC              
RsaI          548  GTAC                 GT^AC
             1144  GTAC                
Sau3AI        274  GATC                 ^GATC
              430  GATC                
Sau96I       1035  GGACC                G^G[ACGT]CC
             1090  GGACC               
SphI           55  GCATGC               GCATG^C
              458  GCATGC              
TaqI         1064  TCGA                 T^CGA
             1179  TCGA                
TseI          976  GCTGC                G^C[AT]GC
             1098  GCAGC               
TthHB8I      1064  TCGA                 T^CGA
             1179  TCGA                
XmnI          417  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              439  GAAGAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AcsI        AluI        ApoI        BbsI        BcnI        
BpuAI       BsaJI       BsmAI       BssKI       BstF5I      
BstNI       Cac8I       DdeI        EcoRII      EcoT22I     
FokI        HapII       HinfI       HpaII       MaeIII      
MboII       MnlI        MseI        MspI        MvaI        
NciI        NlaIII      NsiI        PleI        ScrFI       
SfaNI       SmlI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       
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