Restriction map for pF1KB6667
 (Linear) (Six-base) MAPSORT of: KIBB6667.seq  Check: 4563  from: 1  to: 1275



KIBB6667.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     875    1275 
   Size:        350     525     400

  Fragments arranged by size: 

                 525     400     350
 
AfeI AGC'GCT

Cuts at:      0     575    1275 
   Size:        575     700
 
AflII C'TTAA_G

Cuts at:      0     486    1275 
   Size:        486     789
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     406    1275 
   Size:        406     869
 
AlwNI CAG_nnn'CTG

Cuts at:      0     511    1275 
   Size:        511     764
 
ApaLI G'TGCA_C

Cuts at:      0     614    1275 
   Size:        614     661
 
ApoI r'AATT_y

Cuts at:      0     195     442     691     802     866    1189    1275 
   Size:        195     247     249     111      64     323      86

  Fragments arranged by size: 

                 323     249     247     195     111      86      64
 
AvaI C'yCGr_G

Cuts at:      0    1071    1178    1275 
   Size:       1071     107      97

  Fragments arranged by size: 

                1071     107      97
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     936     969    1275 
   Size:        936      33     306

  Fragments arranged by size: 

                 936     306      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     245     339     424     671    1275 
   Size:        245      94      85     247     604

  Fragments arranged by size: 

                 604     247     245      94      85
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1159    1193    1275 
   Size:       1159      34      82

  Fragments arranged by size: 

                1159      82      34
 
BclI T'GATC_A

Cuts at:      0     273    1275 
   Size:        273    1002
 
BfrBI ATG'CAT

Cuts at:      0      59     458    1006    1275 
   Size:         59     399     548     269

  Fragments arranged by size: 

                 548     399     269      59
 
Bme1580I G_kGCm'C

Cuts at:      0     618    1275 
   Size:        618     657
 
BmgBI CAC'GTC

Cuts at:      0     779    1275 
   Size:        779     496
 
BmtI G_CTAG'C

Cuts at:      0     466    1275 
   Size:        466     809
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     731    1013    1275 
   Size:        731     282     262

  Fragments arranged by size: 

                 731     282     262
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188    1275 
   Size:        188    1087
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     747     989    1275 
   Size:        747     242     286

  Fragments arranged by size: 

                 747     286     242
 
BsaAI yAC'GTr

Cuts at:      0     124    1275 
   Size:        124    1151
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672    1275 
   Size:        672     603
 
BsiHKAI G_wGCw'C

Cuts at:      0     532     618    1275 
   Size:        532      86     657

  Fragments arranged by size: 

                 657     532      86
 
Bsp1286I G_dGCh'C

Cuts at:      0     532     618    1275 
   Size:        532      86     657

  Fragments arranged by size: 

                 657     532      86
 
BstYI r'GATC_y

Cuts at:      0     429    1275 
   Size:        429     846
 
Bsu36I CC'TnA_GG

Cuts at:      0     587    1275 
   Size:        587     688
 
BtsI GCAGTG_nn'

Cuts at:      0     509     794    1275 
   Size:        509     285     481

  Fragments arranged by size: 

                 509     481     285
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     731     875    1013    1275 
   Size:        350     381     144     138     262

  Fragments arranged by size: 

                 381     350     262     144     138
 
EcoRI G'AATT_C

Cuts at:      0     195    1189    1275 
   Size:        195     994      86

  Fragments arranged by size: 

                 994     195      86
 
HaeII r_GCGC'y

Cuts at:      0     577    1275 
   Size:        577     698
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     258     290     709     741     746     778    1120    1152 
   Size:        258      32     419      32       5      32     342      32

Cuts at:   1152    1275 
   Size:        123

  Fragments arranged by size: 

                 419     342     258     123      32      32      32      32
                   5
 
MslI CAynn'nnrTG

Cuts at:      0    1262    1275 
   Size:       1262      13
 
MspA1I CmG'CkG

Cuts at:      0     141    1275 
   Size:        141    1134
 
NheI G'CTAG_C

Cuts at:      0     462    1275 
   Size:        462     813
 
NsiI A_TGCA'T

Cuts at:      0      61     460    1008    1275 
   Size:         61     399     548     267

  Fragments arranged by size: 

                 548     399     267      61
 
NspI r_CATG'y

Cuts at:      0      59     462    1275 
   Size:         59     403     813

  Fragments arranged by size: 

                 813     403      59
 
PasI CC'CwG_GG

Cuts at:      0     829    1275 
   Size:        829     446
 
PspXI vC'TCGA_Gb

Cuts at:      0    1178    1275 
   Size:       1178      97
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1157    1189    1275 
   Size:       1157      32      86

  Fragments arranged by size: 

                1157      86      32
 
PstI C_TGCA'G

Cuts at:      0      78    1275 
   Size:         78    1197
 
PvuII CAG'CTG

Cuts at:      0     141    1275 
   Size:        141    1134
 
SfcI C'TryA_G

Cuts at:      0      74    1275 
   Size:         74    1201
 
SmaI CCC'GGG

Cuts at:      0    1073    1275 
   Size:       1073     202
 
SmlI C'TyrA_G

Cuts at:      0     203     486    1178    1275 
   Size:        203     283     692      97

  Fragments arranged by size: 

                 692     283     203      97
 
SphI G_CATG'C

Cuts at:      0      59     462    1275 
   Size:         59     403     813

  Fragments arranged by size: 

                 813     403      59
 
XhoI C'TCGA_G

Cuts at:      0    1178    1275 
   Size:       1178      97
 
XmaI C'CCGG_G

Cuts at:      0    1071    1275 
   Size:       1071     204
 
XmnI GAAnn'nnTTC

Cuts at:      0     421     443    1275 
   Size:        421      22     832

  Fragments arranged by size: 

                 832     421      22
 

 Enzymes that do cut:

     AcuI     AfeI    AflII     AlfI    AlwNI    ApaLI     ApoI     AvaI
     BaeI     BbsI     BcgI     BclI    BfrBI Bme1580I    BmgBI     BmtI
     BpmI    BpuEI     BsaI    BsaAI     BsgI  BsiHKAI Bsp1286I    BstYI
   Bsu36I     BtsI  Eco57MI    EcoRI    HaeII    Hin4I     MslI   MspA1I
     NheI     NsiI     NspI     PasI    PspXI     PsrI     PstI    PvuII
     SfcI     SmaI     SmlI     SphI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI
     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI     BglI    BglII     BlpI
     BmrI     BplI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     ZraI
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